Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INR79_RS02445 Genome accession   NZ_CP063199
Coordinates   511671..513359 (-) Length   562 a.a.
NCBI ID   WP_223095887.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43132     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 506671..518359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INR79_RS02410 (INR79_02410) rplS 506811..507164 (+) 354 WP_004408890.1 50S ribosomal protein L19 -
  INR79_RS02415 (INR79_02415) mutT 507352..507753 (+) 402 WP_022599630.1 8-oxo-dGTP diphosphatase MutT -
  INR79_RS02420 (INR79_02420) yacG 507840..508034 (-) 195 WP_022549572.1 DNA gyrase inhibitor YacG -
  INR79_RS02425 (INR79_02425) zapD 508091..508831 (-) 741 WP_223095880.1 cell division protein ZapD -
  INR79_RS02430 (INR79_02430) coaE 508864..509469 (-) 606 WP_022599625.1 dephospho-CoA kinase -
  INR79_RS02435 (INR79_02435) pilD 509519..510388 (-) 870 WP_045966127.1 prepilin peptidase Machinery gene
  INR79_RS02440 (INR79_02440) pilC 510429..511661 (-) 1233 WP_223095884.1 type II secretion system F family protein Machinery gene
  INR79_RS02445 (INR79_02445) pilB 511671..513359 (-) 1689 WP_223095887.1 type IV-A pilus assembly ATPase PilB Machinery gene
  INR79_RS02450 (INR79_02450) pilA 513368..513778 (-) 411 WP_262415375.1 pilin Machinery gene
  INR79_RS02455 (INR79_02455) nadC 514056..514943 (-) 888 WP_223095892.1 carboxylating nicotinate-nucleotide diphosphorylase -
  INR79_RS02460 (INR79_02460) ampD 515072..515629 (+) 558 WP_223095894.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INR79_RS02465 (INR79_02465) pdhR 515942..516712 (+) 771 WP_004397664.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62134.91 Da        Isoelectric Point: 4.7880

>NTDB_id=493801 INR79_RS02445 WP_223095887.1 511671..513359(-) (pilB) [Vibrio sp. SCSIO 43132]
MHTNLASILRQADIISEEHEQAIVERIESSGGSVPGAILALGILPSFELTQHIAEIFGLSVIETNQYDYTQLCEQLGLRD
LITRYNALPVESTNNTLFIALSDPTVVEAEEEFRFATNQQVEILLCDERELTSCIRRMYGKNRVESGTGGKEITQDELAD
LVQISDDEFESVEDLSQDDAPVSRFIHQVLLDAVRKGASDIHFEPYEDTYRIRMRCDGILIQANSPAPQLGRRLAARLKI
LSKLDIAERRLPQDGRIKLRLSEKAAIDLRVSTLPTLWGEKVVLRLLDSSSANLNIDMLGYSAEQKALYLEALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNTVERNISTAEDPVEINLAGINQVQVSPKIGFTFAAALKSFLRQDPDIVMLGEIRDFET
AEIAIKAAQTGHLVLSTLHTNSAAETVIRLGNMGVEPYNLASSLSLIIAQRLARRLCPHCKQPHQLPSTVLEKHLIPEDT
TIYQASAEGCADCNAGYSGRIGIYEVMPFDTDMQQAISHKASIVEIESLAVKNGMKTLSQSGIEILKQGITSYQELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=493801 INR79_RS02445 WP_223095887.1 511671..513359(-) (pilB) [Vibrio sp. SCSIO 43132]
ATGCATACCAACTTAGCTAGCATTCTTCGCCAAGCTGATATTATTAGCGAAGAACATGAGCAAGCTATAGTCGAACGCAT
TGAAAGTTCCGGGGGCAGTGTCCCCGGAGCTATTCTTGCTCTTGGCATTCTTCCTTCTTTTGAGCTGACTCAGCATATTG
CTGAGATTTTTGGTTTATCGGTCATCGAAACCAACCAATACGATTATACTCAGCTATGTGAGCAGTTAGGACTTCGAGAC
TTAATTACCCGTTACAACGCGTTACCCGTTGAATCCACTAATAACACGCTTTTTATTGCTTTATCGGACCCTACTGTCGT
TGAAGCAGAAGAGGAATTTCGCTTTGCAACTAACCAACAAGTAGAAATATTGCTCTGCGACGAAAGAGAGCTTACAAGCT
GTATTCGTCGCATGTATGGTAAGAATCGAGTTGAAAGCGGAACTGGTGGTAAAGAGATCACTCAAGACGAGTTAGCAGAC
CTGGTTCAAATCAGTGACGATGAATTTGAGTCTGTAGAAGACTTGAGTCAGGATGACGCCCCCGTAAGTCGTTTTATTCA
CCAGGTCCTTCTGGACGCTGTACGTAAAGGTGCATCGGATATTCACTTCGAGCCTTACGAAGACACATACCGCATCCGTA
TGCGATGCGATGGAATACTGATTCAGGCCAATAGCCCTGCTCCACAGCTCGGACGGCGTTTGGCTGCTAGGCTCAAAATT
CTTTCTAAACTTGATATTGCCGAACGCCGACTTCCACAAGATGGTCGTATCAAACTCAGGCTAAGCGAAAAAGCCGCCAT
CGATCTGCGTGTATCCACACTGCCTACCCTATGGGGGGAGAAGGTGGTGCTTCGGTTATTGGACAGCAGCAGTGCTAATT
TAAACATAGATATGCTTGGTTACAGTGCTGAGCAAAAAGCCCTTTATTTAGAAGCTTTACGCCGCCCCCAAGGAATGATT
CTGATGACGGGACCTACTGGTAGCGGTAAGACCGTATCCTTGTACACCGGCTTAAGTATCCTCAACACCGTTGAAAGAAA
CATTTCCACAGCCGAAGACCCTGTCGAAATCAACCTTGCCGGAATCAATCAGGTTCAGGTTAGCCCTAAGATTGGGTTTA
CCTTTGCCGCAGCACTTAAGTCCTTTCTTCGACAAGATCCCGATATTGTTATGCTTGGAGAAATTCGAGATTTCGAAACC
GCTGAAATTGCTATCAAGGCAGCGCAAACGGGTCACTTAGTGCTTTCTACATTACATACCAATTCTGCGGCTGAAACCGT
TATTCGACTCGGTAATATGGGAGTTGAGCCATACAACTTAGCCTCATCTCTGAGCCTCATTATTGCTCAAAGGCTCGCAA
GGCGGCTGTGCCCTCATTGCAAACAACCCCATCAATTACCTTCTACTGTATTGGAAAAACATTTAATCCCAGAAGACACC
ACTATTTATCAAGCTTCTGCTGAAGGCTGCGCAGACTGCAATGCGGGTTATAGCGGGCGTATAGGGATCTATGAAGTCAT
GCCGTTTGATACCGATATGCAGCAAGCCATTTCTCACAAAGCGAGTATTGTGGAGATAGAAAGCCTTGCAGTTAAAAATG
GAATGAAAACATTAAGCCAGTCTGGAATTGAGATCCTCAAACAAGGAATAACCAGTTATCAAGAACTCCAACGCGTTCTG
TATCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

69.094

100

0.692
  pilB Vibrio campbellii strain DS40M4

67.082

100

0.671
  pilB Vibrio parahaemolyticus RIMD 2210633

66.607

100

0.667
  pilB Acinetobacter baumannii D1279779

50.954

93.238

0.475
  pilB Acinetobacter baylyi ADP1

50.677

91.993

0.466
  pilB Legionella pneumophila strain ERS1305867

47.112

98.577

0.464
  pilF Neisseria gonorrhoeae MS11

45.39

100

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

34.609

100

0.37
  pilF Thermus thermophilus HB27

36.879

100

0.37