Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   INR79_RS01360 Genome accession   NZ_CP063199
Coordinates   305736..307478 (+) Length   580 a.a.
NCBI ID   WP_004405324.1    Uniprot ID   A0AAV2VJ55
Organism   Vibrio sp. SCSIO 43132     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 300736..312478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INR79_RS01340 (INR79_01340) pilM 303041..304057 (+) 1017 WP_223095699.1 type IV pilus assembly protein PilM Machinery gene
  INR79_RS01345 (INR79_01345) pilN 304050..304610 (+) 561 WP_223095701.1 PilN domain-containing protein Machinery gene
  INR79_RS01350 (INR79_01350) pilO 304607..305194 (+) 588 WP_022593067.1 type 4a pilus biogenesis protein PilO Machinery gene
  INR79_RS01355 (INR79_01355) pilP 305187..305699 (+) 513 WP_022603536.1 pilus assembly protein PilP Machinery gene
  INR79_RS01360 (INR79_01360) pilQ 305736..307478 (+) 1743 WP_004405324.1 type IV pilus secretin PilQ Machinery gene
  INR79_RS01365 (INR79_01365) aroK 307668..308186 (+) 519 WP_004405322.1 shikimate kinase AroK -
  INR79_RS01370 (INR79_01370) aroB 308209..309297 (+) 1089 WP_045962995.1 3-dehydroquinate synthase -
  INR79_RS01375 (INR79_01375) - 309328..310851 (+) 1524 WP_223095703.1 AAA family ATPase -
  INR79_RS01380 (INR79_01380) - 310921..311745 (+) 825 WP_022596942.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  INR79_RS01385 (INR79_01385) - 311735..312436 (+) 702 WP_223095705.1 DNA-methyltransferase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62667.93 Da        Isoelectric Point: 4.3014

>NTDB_id=493790 INR79_RS01360 WP_004405324.1 305736..307478(+) (pilQ) [Vibrio sp. SCSIO 43132]
MAEFCRAHIYRCALLIFIGLFSTTGVHAQSNEFVGIDFRLSKEKHALLVVELASNAAAVDIQPMPDGLNISLHETSVVDE
KLFVVDVSDFATSVENVEVFREGSNTRLFASTNAAYNYDYRLRDRFLEITVSAKPKDEPEQASILEKEGKLISINFQDIP
VRSVLQLIADYNGFNLVVSDSVTGNLTLKVDDVPWQQVLDIILKVKGLDKRVEGNVILVAPKEEFDQREQQDLEQERLAQ
ELGTLESKIVEVKFAKASDIAEMIGGDGEISMLSERGSISIDERTNSLLVRDLPENISVIEDIVESLDIPVKQVQIEARI
VTVTEGELEELGVRWGFSSTSGPNSVGATIENNLATVGLYQAGGSGGDSGGEGGGEGGGSTSIDDFLAVNLGATNANAAS
IAFQVAKLGSDTLLDLELSALQTENKAEIISSPRLITTNKKPAYIEQGTEIPYLEASSSGATTVAFRKAVLSLKVTPQIT
PDNKLVLDLSVTQDRPGKVVKTGNGEALAIETQRIGTQVLVDNGETVVLGGIYQQSVTNTVDKVPLLGDLPLLGALFRRS
YENLGKSELLIFITPKVVLQ

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=493790 INR79_RS01360 WP_004405324.1 305736..307478(+) (pilQ) [Vibrio sp. SCSIO 43132]
ATGGCGGAGTTTTGTCGGGCGCATATATATCGTTGTGCTCTGCTTATCTTTATCGGGTTGTTTTCGACAACCGGGGTTCA
TGCCCAGTCCAACGAATTCGTTGGTATCGATTTTAGGCTCAGTAAAGAAAAACATGCTCTGCTGGTCGTGGAGTTAGCAT
CGAATGCGGCAGCGGTAGACATTCAGCCGATGCCAGATGGCTTAAACATCTCCCTGCATGAAACCAGCGTGGTGGACGAA
AAGCTTTTCGTGGTCGACGTATCCGATTTTGCAACTAGCGTAGAAAATGTGGAAGTGTTCCGTGAGGGCTCTAACACCCG
TTTATTTGCCAGCACAAACGCGGCTTATAACTACGACTACCGCTTACGGGATCGTTTTCTGGAAATCACCGTCTCTGCGA
AACCCAAAGACGAACCAGAGCAGGCGAGCATTCTAGAAAAAGAAGGCAAGCTCATTTCTATTAACTTCCAGGACATCCCT
GTTCGAAGCGTATTACAGCTTATCGCTGACTATAACGGGTTCAATCTGGTAGTTTCTGACTCAGTCACAGGCAACTTAAC
GCTTAAGGTGGATGATGTACCGTGGCAGCAAGTGCTAGACATCATCCTGAAAGTAAAAGGGTTGGATAAGCGTGTTGAAG
GCAATGTAATTCTGGTTGCCCCAAAAGAAGAGTTTGACCAAAGAGAGCAGCAAGATCTTGAACAAGAACGCTTGGCGCAA
GAGCTCGGTACGCTGGAATCCAAAATCGTTGAAGTAAAATTTGCCAAAGCGTCTGACATTGCGGAAATGATAGGTGGCGA
CGGTGAAATTTCCATGCTTTCTGAGCGCGGAAGTATTTCCATTGATGAACGTACCAATTCCCTTCTGGTACGCGACTTGC
CGGAAAACATCAGCGTTATTGAAGATATTGTGGAATCTCTCGATATCCCTGTTAAACAGGTTCAGATAGAAGCTCGTATT
GTGACTGTCACAGAAGGTGAACTTGAAGAGTTGGGTGTCCGCTGGGGCTTCAGTTCAACAAGTGGACCAAACTCTGTGGG
CGCGACCATTGAAAATAACCTCGCGACGGTCGGTCTTTATCAGGCTGGTGGCTCAGGTGGTGATTCTGGAGGCGAAGGCG
GCGGTGAAGGTGGAGGCAGCACCAGTATTGATGACTTCCTTGCCGTCAACCTGGGGGCGACAAATGCCAATGCAGCCAGT
ATCGCGTTCCAAGTGGCAAAACTTGGCTCTGACACCCTGTTGGATTTGGAGCTGTCTGCTCTGCAAACGGAAAACAAAGC
GGAAATTATCTCCAGCCCGAGACTGATTACCACCAACAAGAAACCGGCCTACATCGAGCAAGGTACTGAAATTCCTTACC
TTGAAGCTTCATCCAGTGGTGCAACTACAGTCGCATTCCGTAAGGCTGTGCTGAGCTTAAAGGTGACGCCACAGATTACC
CCAGACAACAAACTGGTGCTGGATTTGAGTGTTACTCAGGACAGACCCGGCAAAGTCGTAAAAACAGGCAATGGTGAAGC
CCTGGCCATTGAAACTCAAAGAATCGGTACTCAGGTGTTGGTAGACAATGGAGAAACGGTGGTTCTTGGGGGTATTTATC
AACAAAGTGTGACAAATACGGTCGATAAAGTGCCACTTTTGGGTGATTTACCTCTGTTAGGTGCACTTTTCCGTCGAAGC
TACGAAAATTTAGGAAAAAGCGAACTCCTTATTTTCATTACGCCAAAGGTCGTTCTACAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(420-578)

Bacterial type II/III secretion system short domain (Secretin_N)

(248-313)

Secretin and TonB N terminus short domain (STN)

(174-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

72.432

95.69

0.693
  pilQ Vibrio cholerae strain A1552

66.44

100

0.672
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

66.44

100

0.672
  pilQ Legionella pneumophila strain ERS1305867

40.564

97.759

0.397
  pilQ Legionella pneumophila strain Lp02

40.564

97.759

0.397
  pilQ Pseudomonas aeruginosa PAK

40.108

95.862

0.384
  comQ Acinetobacter baylyi ADP1

38.179

98.448

0.376
  pilQ Acinetobacter baumannii D1279779

36.409

100

0.374
  pilQ Acinetobacter baumannii strain A118

36.409

100

0.374