Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   INR79_RS01340 Genome accession   NZ_CP063199
Coordinates   303041..304057 (+) Length   338 a.a.
NCBI ID   WP_223095699.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43132     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 298041..309057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INR79_RS01325 (INR79_01325) - 298550..299281 (-) 732 WP_004405337.1 glutathione peroxidase -
  INR79_RS01330 (INR79_01330) oxyR 299460..300341 (+) 882 WP_004405335.1 DNA-binding transcriptional regulator OxyR -
  INR79_RS01335 (INR79_01335) - 300397..302895 (-) 2499 WP_004405333.1 penicillin-binding protein 1A -
  INR79_RS01340 (INR79_01340) pilM 303041..304057 (+) 1017 WP_223095699.1 type IV pilus assembly protein PilM Machinery gene
  INR79_RS01345 (INR79_01345) pilN 304050..304610 (+) 561 WP_223095701.1 PilN domain-containing protein Machinery gene
  INR79_RS01350 (INR79_01350) pilO 304607..305194 (+) 588 WP_022593067.1 type 4a pilus biogenesis protein PilO Machinery gene
  INR79_RS01355 (INR79_01355) pilP 305187..305699 (+) 513 WP_022603536.1 pilus assembly protein PilP Machinery gene
  INR79_RS01360 (INR79_01360) pilQ 305736..307478 (+) 1743 WP_004405324.1 type IV pilus secretin PilQ Machinery gene
  INR79_RS01365 (INR79_01365) aroK 307668..308186 (+) 519 WP_004405322.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37108.40 Da        Isoelectric Point: 6.4160

>NTDB_id=493786 INR79_RS01340 WP_223095699.1 303041..304057(+) (pilM) [Vibrio sp. SCSIO 43132]
MGRSLVVGIDFGHHSIKAVAMKPIGELMQLVGYNELTVPEDIFSENHTLDYQKIVKKLKELKKGLPLFSQQVAVSVPDKA
VISKVLQIEQDMDGREQEFAVHQAFGQQSPIPLEELSLDFFEVDQKVTTRSTTSTFQVYATRKEVVDSRVAALTKAGLKP
LTVDVHANSLLKVWRLASQVHPERRQWMLVDIGLNQTSLCTVAAGRTPFYKEVPFGTQNVAQSQEVSESISLSSDSLTQG
FVRELGEKLQRHISLYNSVNPQQNVDGIWISGGGANLVGIADMLSIELSLEVAQLNPFGLVENGTKKSIKQLSDSAAYGV
AMGLAISGVEWLKGNHHA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=493786 INR79_RS01340 WP_223095699.1 303041..304057(+) (pilM) [Vibrio sp. SCSIO 43132]
ATGGGTAGATCTTTAGTAGTTGGCATCGATTTCGGTCATCACAGTATCAAAGCTGTCGCCATGAAACCCATTGGCGAGTT
AATGCAACTTGTTGGTTATAATGAGCTTACTGTACCCGAAGATATTTTCTCTGAAAACCATACGCTCGATTATCAGAAGA
TTGTAAAGAAACTCAAAGAACTTAAAAAGGGTTTGCCTCTTTTTAGTCAACAAGTCGCAGTTTCCGTACCAGACAAGGCT
GTTATCAGCAAAGTTCTGCAAATTGAACAGGATATGGATGGCAGAGAACAAGAGTTTGCCGTTCATCAGGCTTTTGGTCA
GCAGTCTCCCATTCCACTGGAAGAGCTGAGTTTAGACTTCTTCGAAGTGGACCAAAAAGTCACCACGCGCAGCACCACAT
CTACCTTTCAGGTTTATGCAACTCGAAAAGAGGTGGTTGATTCGCGAGTTGCGGCATTAACGAAGGCTGGGTTAAAACCG
TTGACGGTTGATGTGCACGCCAACAGCCTGCTAAAAGTCTGGCGTCTTGCCAGCCAGGTTCATCCGGAACGCCGACAATG
GATGCTGGTGGATATCGGATTGAATCAAACGTCTTTATGCACGGTTGCCGCGGGAAGAACCCCTTTCTATAAAGAAGTGC
CTTTCGGTACTCAGAACGTGGCTCAGTCACAAGAAGTTTCTGAATCCATCTCTTTAAGCAGCGATTCTCTTACACAAGGG
TTTGTCAGAGAGTTAGGCGAAAAGCTGCAGCGCCATATTAGCCTTTATAACTCCGTTAATCCTCAGCAAAATGTCGACGG
TATCTGGATCAGTGGTGGTGGTGCCAATCTGGTTGGTATTGCGGACATGCTAAGCATCGAACTTTCTCTGGAAGTAGCAC
AGCTGAATCCGTTTGGTCTAGTGGAAAACGGCACTAAGAAGTCGATAAAGCAGTTATCTGATTCTGCCGCGTATGGTGTG
GCCATGGGGCTGGCAATCAGTGGAGTAGAATGGCTGAAAGGAAATCACCATGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-223)

Type IV pilus assembly protein PilM; (PilM_2)

(239-327)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.412

100

0.547
  pilM Vibrio campbellii strain DS40M4

44.379

100

0.444