Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   INR79_RS01345 Genome accession   NZ_CP063199
Coordinates   304050..304610 (+) Length   186 a.a.
NCBI ID   WP_223095701.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43132     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 299050..309610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INR79_RS01330 (INR79_01330) oxyR 299460..300341 (+) 882 WP_004405335.1 DNA-binding transcriptional regulator OxyR -
  INR79_RS01335 (INR79_01335) - 300397..302895 (-) 2499 WP_004405333.1 penicillin-binding protein 1A -
  INR79_RS01340 (INR79_01340) pilM 303041..304057 (+) 1017 WP_223095699.1 type IV pilus assembly protein PilM Machinery gene
  INR79_RS01345 (INR79_01345) pilN 304050..304610 (+) 561 WP_223095701.1 PilN domain-containing protein Machinery gene
  INR79_RS01350 (INR79_01350) pilO 304607..305194 (+) 588 WP_022593067.1 type 4a pilus biogenesis protein PilO Machinery gene
  INR79_RS01355 (INR79_01355) pilP 305187..305699 (+) 513 WP_022603536.1 pilus assembly protein PilP Machinery gene
  INR79_RS01360 (INR79_01360) pilQ 305736..307478 (+) 1743 WP_004405324.1 type IV pilus secretin PilQ Machinery gene
  INR79_RS01365 (INR79_01365) aroK 307668..308186 (+) 519 WP_004405322.1 shikimate kinase AroK -
  INR79_RS01370 (INR79_01370) aroB 308209..309297 (+) 1089 WP_045962995.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 186 a.a.        Molecular weight: 21319.42 Da        Isoelectric Point: 7.4656

>NTDB_id=493787 INR79_RS01345 WP_223095701.1 304050..304610(+) (pilN) [Vibrio sp. SCSIO 43132]
MHSVNLLPWRDEMRARYRRQFFSYLAAAILAAVGVQWGVGVYFSQQMSVQEERNAELARHISYLDQQLRGLSEVRDQHES
ILTRLRSVEALQIKRNKTTDFMSHLPDTIPPGVYVDKIEMNDFEVEVNGISDSTANITTMLDSMERSAKLADVEMHSIVS
GKKLFGKVFKTFKVSFIFAPIKEDKS

Nucleotide


Download         Length: 561 bp        

>NTDB_id=493787 INR79_RS01345 WP_223095701.1 304050..304610(+) (pilN) [Vibrio sp. SCSIO 43132]
ATGCATAGCGTTAACTTACTGCCCTGGCGTGATGAAATGCGCGCCAGATACCGCCGCCAGTTTTTTTCTTATCTGGCTGC
CGCGATACTCGCCGCAGTCGGAGTGCAGTGGGGCGTTGGCGTTTATTTCTCGCAGCAAATGAGTGTTCAGGAAGAACGCA
ATGCTGAGCTGGCTCGTCACATCTCCTACTTAGACCAGCAACTACGCGGTTTGAGTGAGGTTCGCGATCAGCACGAGTCT
ATTCTTACCCGACTGCGCTCGGTCGAGGCGCTTCAAATCAAGCGTAACAAAACCACGGATTTCATGTCCCACCTGCCAGA
TACTATTCCACCGGGTGTTTACGTCGACAAAATTGAAATGAACGATTTTGAAGTTGAAGTGAATGGCATCAGTGACAGCA
CAGCCAATATCACCACAATGCTCGACAGTATGGAGCGCTCCGCCAAGCTGGCAGATGTGGAAATGCACTCCATTGTTTCG
GGCAAGAAGTTGTTTGGCAAAGTATTCAAAACCTTCAAAGTATCCTTCATTTTTGCGCCTATCAAGGAAGACAAATCATG
A

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-162)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

52.941

100

0.532
  pilN Vibrio cholerae strain A1552

53.591

97.312

0.522