Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   INR79_RS01350 Genome accession   NZ_CP063199
Coordinates   304607..305194 (+) Length   195 a.a.
NCBI ID   WP_022593067.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43132     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 299607..310194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INR79_RS01335 (INR79_01335) - 300397..302895 (-) 2499 WP_004405333.1 penicillin-binding protein 1A -
  INR79_RS01340 (INR79_01340) pilM 303041..304057 (+) 1017 WP_223095699.1 type IV pilus assembly protein PilM Machinery gene
  INR79_RS01345 (INR79_01345) pilN 304050..304610 (+) 561 WP_223095701.1 PilN domain-containing protein Machinery gene
  INR79_RS01350 (INR79_01350) pilO 304607..305194 (+) 588 WP_022593067.1 type 4a pilus biogenesis protein PilO Machinery gene
  INR79_RS01355 (INR79_01355) pilP 305187..305699 (+) 513 WP_022603536.1 pilus assembly protein PilP Machinery gene
  INR79_RS01360 (INR79_01360) pilQ 305736..307478 (+) 1743 WP_004405324.1 type IV pilus secretin PilQ Machinery gene
  INR79_RS01365 (INR79_01365) aroK 307668..308186 (+) 519 WP_004405322.1 shikimate kinase AroK -
  INR79_RS01370 (INR79_01370) aroB 308209..309297 (+) 1089 WP_045962995.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 195 a.a.        Molecular weight: 22722.10 Da        Isoelectric Point: 4.6739

>NTDB_id=493788 INR79_RS01350 WP_022593067.1 304607..305194(+) (pilO) [Vibrio sp. SCSIO 43132]
MIDWQELDFEDLPEWPLVAQVLVLALLFLILQGVGSWFYLSPMKADLEQLKQKEQTLKSTLIIKTNKVAALPKLQEQLDE
LNSRYDYLLKQLPEQKELASMLASVNELGIQHGLTFTRIDWGKKQNQEFLYRLPLNIELTGTYENIGKFSAAIANLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKPEVTDE

Nucleotide


Download         Length: 588 bp        

>NTDB_id=493788 INR79_RS01350 WP_022593067.1 304607..305194(+) (pilO) [Vibrio sp. SCSIO 43132]
ATGATCGATTGGCAAGAACTTGATTTCGAAGACTTACCGGAATGGCCGCTTGTTGCACAGGTTTTAGTGCTGGCGCTGTT
GTTTCTGATTCTTCAAGGGGTGGGCAGCTGGTTTTATCTCTCTCCCATGAAAGCCGATCTTGAGCAGCTCAAGCAGAAAG
AGCAGACACTCAAAAGCACCCTCATTATCAAAACCAATAAGGTAGCGGCACTGCCAAAATTGCAGGAGCAGCTGGATGAG
CTCAATAGCCGTTATGACTATCTGTTAAAACAGCTTCCTGAACAGAAAGAGTTGGCAAGTATGCTGGCTTCTGTAAACGA
GCTGGGTATTCAGCATGGCTTGACTTTTACCCGTATCGATTGGGGTAAGAAGCAGAATCAGGAGTTTCTTTATCGTCTGC
CTTTGAATATTGAACTCACCGGAACTTACGAAAACATTGGTAAGTTCTCAGCGGCCATTGCCAACCTACCGCGGATCATA
AACTTCGACGATGTTGACTGGCAGCGGGTCAGTCAGGAAAGCAGCACCCTTCACTTCAGGGTAAGGGCGTACACCTATCA
GTTCAAACCGGAGGTGACCGATGAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

68.718

100

0.687
  pilO Vibrio campbellii strain DS40M4

66.316

97.436

0.646