NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	INP92_RS00255	Genome accession	NZ_CP063122
Coordinates	53300..53743 (+)	Length	147 a.a.
NCBI ID	WP_197544812.1	Uniprot ID	-
Organism	Haemophilus parainfluenzae strain M1C125_4
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 48300..58743

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
INP92_RS00225 (INP92_00225)	tadA	48515..49033 (-)	519	WP_152682592.1	tRNA adenosine(34) deaminase TadA	-
INP92_RS00230 (INP92_00230)	-	49043..49894 (-)	852	WP_197554228.1	thymidylate synthase	-
INP92_RS00235 (INP92_00235)	lgt	49905..50714 (-)	810	WP_197554231.1	prolipoprotein diacylglyceryl transferase	-
INP92_RS00240 (INP92_00240)	-	50723..51517 (-)	795	WP_197554234.1	sulfite exporter TauE/SafE family protein	-
INP92_RS00245 (INP92_00245)	rppH	51517..52110 (-)	594	WP_197554237.1	RNA pyrophosphohydrolase	-
INP92_RS00250 (INP92_00250)	ampD	52594..53157 (-)	564	WP_197544811.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
INP92_RS00255 (INP92_00255)	pilA	53300..53743 (+)	444	WP_197544812.1	prepilin peptidase-dependent pilin	Machinery gene
INP92_RS00260 (INP92_00260)	pilB	53750..55129 (+)	1380	WP_197544813.1	GspE/PulE family protein	Machinery gene
INP92_RS00265 (INP92_00265)	pilC	55138..56361 (+)	1224	WP_197554240.1	type II secretion system F family protein	Machinery gene
INP92_RS00270 (INP92_00270)	pilD	56358..57035 (+)	678	WP_197544815.1	prepilin peptidase	Machinery gene
INP92_RS00275 (INP92_00275)	coaE	57124..57744 (+)	621	WP_197544816.1	dephospho-CoA kinase	-
INP92_RS00280 (INP92_00280)	yacG	57737..57943 (+)	207	WP_197544817.1	DNA gyrase inhibitor YacG	-
INP92_RS00285 (INP92_00285)	-	57940..58221 (+)	282	WP_197544818.1	GNAT family N-acetyltransferase	-

Sequence

Protein

Download Length: 147 a.a. Molecular weight: 15141.38 Da Isoelectric Point: 8.4674

                                >NTDB_id=493125 INP92_RS00255 WP_197544812.1 53300..53743(+) (pilA) [Haemophilus parainfluenzae strain M1C125_4]

MKLTFSKPLHKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAISELLQASAPYKSDVELCIYSTNAPTNCSGGSNGIA

ADITTAKGYVKSITTKSGVITVTGNGSLDGISYSLTATGSTSSGVTWTTACPSNADLFPAGFCSSAK

Nucleotide

Download Length: 444 bp

                                >NTDB_id=493125 INP92_RS00255 WP_197544812.1 53300..53743(+) (pilA) [Haemophilus parainfluenzae strain M1C125_4]

ATGAAACTGACTTTTTCTAAACCTTTACATAAAGGTTTTACGTTAATTGAATTGATGATCGTGATTGCGATTATTGCTAT

TCTCGCGACGATCGCCATTCCGTCTTATCAAAATTATACCAAAAAAGCGGCTATCTCCGAATTGTTACAAGCTTCTGCAC

CTTATAAATCTGATGTGGAACTATGTATCTATAGCACCAATGCCCCAACAAACTGTTCAGGTGGCTCAAATGGCATTGCA

GCAGACATCACTACTGCAAAAGGCTATGTCAAATCCATCACCACAAAATCGGGTGTGATTACGGTGACAGGAAATGGTTC

ATTAGATGGGATCAGTTATTCTTTAACGGCGACAGGCTCAACCTCATCAGGTGTCACCTGGACAACTGCTTGCCCAAGCA

ATGCAGATTTATTCCCTGCAGGCTTCTGTTCTTCGGCTAAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	74.658	99.32	0.742
pilA	Haemophilus influenzae Rd KW20	72.603	99.32	0.721
pilA	Glaesserella parasuis strain SC1401	56.757	100	0.571
pilA	Vibrio campbellii strain DS40M4	38.356	99.32	0.381
pilE	Neisseria gonorrhoeae MS11	41.985	89.116	0.374
pilA2	Legionella pneumophila strain ERS1305867	41.406	87.075	0.361
pilA2	Legionella pneumophila str. Paris	41.406	87.075	0.361
pilA	Vibrio cholerae C6706	34.868	100	0.361
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	34.868	100	0.361
pilA	Vibrio cholerae strain A1552	34.868	100	0.361

Detailed information NTG0493125