Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IHE41_RS14750 Genome accession   NZ_CP062153
Coordinates   3159969..3161717 (+) Length   582 a.a.
NCBI ID   WP_005488227.1    Uniprot ID   A0A7H5D3I7
Organism   Vibrio parahaemolyticus strain 17-VB00214     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3154969..3166717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHE41_RS14730 (IHE41_14705) pilM 3157268..3158284 (+) 1017 WP_100662586.1 type IV pilus biogenesis protein PilM Machinery gene
  IHE41_RS14735 (IHE41_14710) pilN 3158268..3158846 (+) 579 WP_264400993.1 PilN domain-containing protein Machinery gene
  IHE41_RS14740 (IHE41_14715) pilO 3158839..3159432 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  IHE41_RS14745 (IHE41_14720) pilP 3159422..3159937 (+) 516 WP_161608959.1 pilus assembly protein PilP Machinery gene
  IHE41_RS14750 (IHE41_14725) pilQ 3159969..3161717 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  IHE41_RS14755 (IHE41_14730) aroK 3161906..3162424 (+) 519 WP_005381319.1 shikimate kinase AroK -
  IHE41_RS14760 (IHE41_14735) aroB 3162456..3163556 (+) 1101 WP_005480443.1 3-dehydroquinate synthase -
  IHE41_RS14765 (IHE41_14740) - 3163609..3165126 (+) 1518 WP_025504650.1 SPOR domain-containing protein -
  IHE41_RS14770 (IHE41_14745) - 3165196..3166035 (+) 840 WP_005486862.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63751.85 Da        Isoelectric Point: 4.4353

>NTDB_id=486515 IHE41_RS14750 WP_005488227.1 3159969..3161717(+) (pilQ) [Vibrio parahaemolyticus strain 17-VB00214]
MKMRQGLTRTPRMLTGLFSLWLFLLLAPLAAAEDVQSNALKSIDFRTNKSKDAVIVVELASPAAIVDVKRVQEGLSIDLM
NTSVKDEQLYLLDVKDFATVVESVEVFRDTSTTRLVAYIDEEYTHDYRLTGRYLEITVSKLKPNEKVPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIAVIREIIESLDIPVK
QVQIEARIVTISEGNMDELGIRWGFTSINGNNTVGGSIENNLATIGLYEGGGDGEGEEGGDGVGIDDFLNVNLAATNPNA
ASIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGKVVKTGTGEAMAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=486515 IHE41_RS14750 WP_005488227.1 3159969..3161717(+) (pilQ) [Vibrio parahaemolyticus strain 17-VB00214]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATGCTTACTGGGCTCTTCTCCTTATGGTTGTTTTTACTTCTAGC
CCCTTTAGCAGCAGCAGAAGATGTACAGTCTAATGCGCTCAAGAGCATCGACTTTCGCACTAACAAAAGCAAAGATGCCG
TGATTGTGGTGGAGCTTGCATCCCCGGCGGCTATTGTTGATGTTAAACGTGTTCAAGAAGGATTAAGCATTGATTTAATG
AACACGTCGGTAAAAGACGAACAGCTTTACCTTCTCGACGTGAAAGATTTCGCGACGGTAGTGGAAAGTGTGGAAGTGTT
CCGCGATACCTCCACCACACGATTAGTTGCGTATATCGATGAAGAATACACGCATGACTATCGTTTGACGGGGCGTTATC
TTGAAATCACTGTCAGCAAGCTTAAACCAAATGAAAAAGTGCCAGACAAGAGCATCTTAGAAAAAGAAGGCAAGCTGATC
TCGATTAACTTCCAAGACATTCCTGTGCGTAACGTTTTGCAGCTGATTGCAGATTACAACCAGTTTAACTTGGTGGTTTC
GGATTCTGTCGAAGGCAACCTTACATTGCGTCTTGATGGTGTGCCGTGGCAGCAAGTGCTCGACATCATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTGGTCGCCCCGACCGATGAGCTGGATTTGCGCGAGAAGCAGCAG
TTAGAAAAACAAAAGCTGACGGAAGAAATGGGGGAACTTTCATCAGAAATCATCAAAGTCAATTTTGCTAAAGCTTCGGA
TATTGCAGAGATGATTAATGGTGAAGGCAACATTAGCATGTTGTCAGAGCGTGGCAGTATGACCATTGATGAGCGAACCA
ATTCGCTATTAATCAGAGAATTGCCAGAGAACATCGCCGTTATTCGCGAAATCATTGAATCGCTCGATATCCCAGTCAAA
CAAGTACAAATTGAAGCCCGAATTGTGACCATCAGTGAAGGTAACATGGACGAGTTAGGTATTCGTTGGGGCTTCACTTC
TATTAATGGTAACAACACCGTTGGTGGCTCTATTGAGAACAACCTAGCGACAATCGGTTTGTACGAAGGTGGTGGTGATG
GCGAAGGTGAAGAGGGTGGTGATGGTGTTGGTATCGATGATTTCCTTAATGTTAATTTAGCCGCGACCAACCCGAATGCG
GCGAGCATTGCGTTCCAAGTGGCAAAGTTAGGTTCTGATACATTATTGGACTTAGAGCTGTCTGCATTGCAGCAAGAATC
AAAAGCAGAAATCATTTCGAGCCCACGATTGATCACGACCAATAAGAAGCCTGCGTACATTGAACAAGGTACCGAAATTC
CTTATCTAGAATCTTCATCGAGTGGTGCAACGTCAGTGACCTTTAAAAAAGCGGTGTTGAGCTTGAAGGTGACACCGCAG
ATCACACCTGACAATCGTTTAGTGTTGGATCTGAGTGTGACCCAAGACCGACCTGGTAAGGTAGTGAAAACGGGCACAGG
AGAGGCAATGGCAATTGATACGCAGCGCATTGGTACCCAAGTTCTGGTGAATAATGGTGAGACGGTTGTGCTCGGTGGTA
TTTTTCAGCACAGCATTACTAACTCAGTGGATAAAGTGCCATTGTTGGGCGATCTTCCGCTATTAGGCGCACTTTTCCGC
CGAAGTTACGAAAACGTTGGCAAAAGTGAGTTGCTAATTTTCGTTACGCCTAAGGTCGTTATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(422-580)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H5D3I7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

91.924

100

0.919
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.408

97.595

0.746
  pilQ Vibrio cholerae strain A1552

76.408

97.595

0.746
  pilQ Legionella pneumophila strain ERS1305867

41.016

94.674

0.388
  pilQ Legionella pneumophila strain Lp02

41.016

94.674

0.388
  pilQ Pseudomonas aeruginosa PAK

40.468

95.533

0.387
  pilQ Acinetobacter baumannii strain A118

37.625

100

0.387
  pilQ Acinetobacter baumannii D1279779

37.625

100

0.387
  comQ Acinetobacter baylyi ADP1

38.25

100

0.383