Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IHE41_RS14740 Genome accession   NZ_CP062153
Coordinates   3158839..3159432 (+) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain 17-VB00214     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3153839..3164432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHE41_RS14725 (IHE41_14700) - 3154555..3157125 (-) 2571 WP_264400992.1 penicillin-binding protein 1A -
  IHE41_RS14730 (IHE41_14705) pilM 3157268..3158284 (+) 1017 WP_100662586.1 type IV pilus biogenesis protein PilM Machinery gene
  IHE41_RS14735 (IHE41_14710) pilN 3158268..3158846 (+) 579 WP_264400993.1 PilN domain-containing protein Machinery gene
  IHE41_RS14740 (IHE41_14715) pilO 3158839..3159432 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  IHE41_RS14745 (IHE41_14720) pilP 3159422..3159937 (+) 516 WP_161608959.1 pilus assembly protein PilP Machinery gene
  IHE41_RS14750 (IHE41_14725) pilQ 3159969..3161717 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  IHE41_RS14755 (IHE41_14730) aroK 3161906..3162424 (+) 519 WP_005381319.1 shikimate kinase AroK -
  IHE41_RS14760 (IHE41_14735) aroB 3162456..3163556 (+) 1101 WP_005480443.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=486513 IHE41_RS14740 WP_005480482.1 3158839..3159432(+) (pilO) [Vibrio parahaemolyticus strain 17-VB00214]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=486513 IHE41_RS14740 WP_005480482.1 3158839..3159432(+) (pilO) [Vibrio parahaemolyticus strain 17-VB00214]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAACTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAGTTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACACTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726