Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IC788_RS13900 Genome accession   NZ_CP061596
Coordinates   2977672..2978709 (+) Length   345 a.a.
NCBI ID   WP_107972265.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2972672..2983709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC788_RS13890 (IC788_13890) - 2975903..2976745 (-) 843 WP_191000605.1 undecaprenyl-diphosphate phosphatase -
  IC788_RS13895 (IC788_13895) - 2976853..2977545 (-) 693 WP_191000606.1 YggS family pyridoxal phosphate-dependent enzyme -
  IC788_RS13900 (IC788_13900) pilT 2977672..2978709 (+) 1038 WP_107972265.1 type IV pilus twitching motility protein PilT Machinery gene
  IC788_RS13905 (IC788_13905) pilU 2978737..2979855 (+) 1119 WP_191000607.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IC788_RS13910 (IC788_13910) fur 2979925..2980362 (-) 438 WP_004703287.1 ferric iron uptake transcriptional regulator -
  IC788_RS13915 (IC788_13915) - 2980474..2980872 (+) 399 WP_004703289.1 outer membrane protein assembly factor BamE -
  IC788_RS13920 (IC788_13920) - 2980928..2981251 (-) 324 WP_191000608.1 RnfH family protein -
  IC788_RS13925 (IC788_13925) - 2981251..2982318 (-) 1068 WP_191000609.1 hypothetical protein -
  IC788_RS13930 (IC788_13930) - 2982471..2982926 (-) 456 WP_004703295.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38404.00 Da        Isoelectric Point: 6.7271

>NTDB_id=483445 IC788_RS13900 WP_107972265.1 2977672..2978709(+) (pilT) [Acinetobacter seifertii strain AS59]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KNLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=483445 IC788_RS13900 WP_107972265.1 2977672..2978709(+) (pilT) [Acinetobacter seifertii strain AS59]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACACTTATCTGCTGGCATGCCACCAAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATTAACTTACCGGCCTTAGAACATAAAGATGTCCACCGCCTTGTTTATGACA
TCATGAACGATAAACAGCGTCGTGACTATGAAGAAAAGCTTGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCACGT
TTCCGTGTGAACGCATTTAACCAAAACCGTGGTGCTGGCGCAGTATTTCGTACCATTCCATCTAAAGTACTTACTATGGA
AGATTTAGGCTTAGGTCAAATCTTTAAGGATATTTGTGACTATCCTCGCGGGATTGTACTGGTGACAGGTCCAACAGGTT
CGGGTAAATCAACCACGCTTGCAGCAATGATGGACTATATTAATGAAAACCGTTATGACCATATTTTAACAGTTGAAGAT
CCTATCGAATTTGTACATCAGTCAAAGAAATGCCTGATTAACCAACGTGAAGTACATCGTGATACACATGGCTTTAATGA
AGCACTTCGTTCAGCACTACGTGAAGACCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACGATTCGATTAG
CACTTACTGCTGCTGAAACGGGTCACTTGGTATTTGGTACGCTTCACACCACGTCAGCTGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCTTTACAAGCTGTTATTTCGCAGAC
TCTACTTAAAAAGAACGGTGGTGGTCGTGTGGCAGCACATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATCC
GTGAAAATAAAGTCGCACAAATGTATTCAGCTATTCAGACGGGTGCCAACCATGGTATGACGACCCTCGACCAAAGCTTA
AAAAACTTGGTGTCTCGTGGTGTAATTAGCCCGCAAACGGCTCGTACTGCTGCAAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

99.42

100

0.994
  pilT Acinetobacter baumannii D1279779

99.13

100

0.991
  pilT Acinetobacter baumannii strain A118

99.13

100

0.991
  pilT Acinetobacter baylyi ADP1

88.372

99.71

0.881
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Pseudomonas stutzeri DSM 10701

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388