Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HP15_RS01395 Genome accession   NC_017506
Coordinates   281044..282165 (+) Length   373 a.a.
NCBI ID   WP_014575888.1    Uniprot ID   E4PL12
Organism   Marinobacter adhaerens HP15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 218645..296130 281044..282165 within 0


Gene organization within MGE regions


Location: 218645..296130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HP15_RS01130 (HP15_235) ubiD 219739..221226 (+) 1488 WP_014575846.1 4-hydroxy-3-polyprenylbenzoate decarboxylase -
  HP15_RS01135 (HP15_236) - 221228..222232 (+) 1005 WP_041644898.1 2Fe-2S iron-sulfur cluster-binding protein -
  HP15_RS01140 (HP15_237) - 222307..223548 (-) 1242 WP_014575848.1 heme biosynthesis HemY N-terminal domain-containing protein -
  HP15_RS01145 (HP15_238) - 223545..224669 (-) 1125 WP_014575849.1 uroporphyrinogen-III C-methyltransferase -
  HP15_RS01150 (HP15_239) - 224678..225487 (-) 810 WP_014575850.1 uroporphyrinogen-III synthase -
  HP15_RS01155 (HP15_240) hemC 225468..226409 (-) 942 WP_014575851.1 hydroxymethylbilane synthase -
  HP15_RS01160 (HP15_241) - 226502..227251 (-) 750 WP_014575852.1 LytTR family DNA-binding domain-containing protein -
  HP15_RS01165 (HP15_242) - 227281..228321 (-) 1041 WP_014575853.1 sensor histidine kinase -
  HP15_RS01170 (HP15_243) argH 228441..229850 (+) 1410 WP_014575854.1 argininosuccinate lyase -
  HP15_RS01175 (HP15_244) - 229878..232322 (-) 2445 WP_014575855.1 EAL domain-containing protein -
  HP15_RS01180 (HP15_245) - 232431..235289 (+) 2859 WP_014575856.1 class I adenylate cyclase -
  HP15_RS21820 (HP15_246) - 235356..235517 (+) 162 WP_227497818.1 LPS translocon maturation chaperone LptM -
  HP15_RS01185 (HP15_247) lysA 235559..236809 (+) 1251 WP_014575857.1 diaminopimelate decarboxylase -
  HP15_RS01190 (HP15_248) dapF 236824..237738 (+) 915 WP_008173211.1 diaminopimelate epimerase -
  HP15_RS01195 (HP15_249) - 237755..238501 (+) 747 WP_008173212.1 DUF484 family protein -
  HP15_RS01200 (HP15_250) xerC 238530..239462 (+) 933 WP_014575858.1 tyrosine recombinase XerC -
  HP15_RS01205 (HP15_251) - 239531..241024 (+) 1494 WP_008173222.1 GGDEF domain-containing protein -
  HP15_RS01210 (HP15_252) - 241018..241251 (-) 234 WP_008173223.1 DUF2789 domain-containing protein -
  HP15_RS01215 (HP15_253) - 241348..244149 (+) 2802 WP_008173225.1 ATP-binding protein -
  HP15_RS01220 (HP15_254) - 244162..244527 (-) 366 WP_008173227.1 hypothetical protein -
  HP15_RS01225 (HP15_255) - 244746..245351 (+) 606 WP_014575862.1 hypothetical protein -
  HP15_RS01230 (HP15_256) - 245359..247575 (+) 2217 WP_014575863.1 patatin-like phospholipase family protein -
  HP15_RS01235 (HP15_257) - 247582..248259 (-) 678 WP_008173234.1 GntR family transcriptional regulator -
  HP15_RS01240 (HP15_258) cls 248343..249773 (-) 1431 WP_014575864.1 cardiolipin synthase -
  HP15_RS01245 (HP15_259) - 249773..250849 (-) 1077 WP_014575865.1 AraC family transcriptional regulator -
  HP15_RS01250 (HP15_260) - 251239..252036 (+) 798 WP_014575867.1 alpha/beta fold hydrolase -
  HP15_RS01255 (HP15_262) - 252138..253223 (+) 1086 WP_014575868.1 SMP-30/gluconolactonase/LRE family protein -
  HP15_RS01260 (HP15_263) - 253223..253684 (+) 462 WP_014575869.1 thioesterase family protein -
  HP15_RS01265 (HP15_264) - 253766..254749 (+) 984 WP_014575870.1 NADP-dependent oxidoreductase -
  HP15_RS01270 (HP15_265) - 254735..256612 (-) 1878 WP_014575871.1 sodium:proton antiporter -
  HP15_RS01275 (HP15_266) tcdA 256634..257425 (-) 792 WP_169702126.1 tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA -
  HP15_RS01280 (HP15_267) - 257698..259473 (-) 1776 WP_014575872.1 acyl-CoA dehydrogenase C-terminal domain-containing protein -
  HP15_RS01285 (HP15_268) - 259644..260387 (+) 744 WP_014575873.1 SDR family oxidoreductase -
  HP15_RS01290 (HP15_269) - 260467..261105 (-) 639 WP_014575874.1 ChrR family anti-sigma-E factor -
  HP15_RS01295 (HP15_270) - 261102..261701 (-) 600 WP_039881993.1 sigma-70 family RNA polymerase sigma factor -
  HP15_RS01300 (HP15_271) - 261941..262978 (+) 1038 WP_014575876.1 AraC family transcriptional regulator -
  HP15_RS01305 (HP15_272) speA 262985..264895 (-) 1911 WP_014575877.1 biosynthetic arginine decarboxylase -
  HP15_RS01310 (HP15_273) speE 265074..265955 (+) 882 WP_014575878.1 polyamine aminopropyltransferase -
  HP15_RS01315 (HP15_274) - 266021..267304 (-) 1284 WP_014575879.1 ammonium transporter -
  HP15_RS01320 (HP15_275) glnK 267379..267717 (-) 339 WP_008173268.1 P-II family nitrogen regulator -
  HP15_RS01325 (HP15_276) - 268099..268353 (+) 255 WP_014575880.1 accessory factor UbiK family protein -
  HP15_RS01330 (HP15_277) comM 268417..269949 (+) 1533 WP_041646072.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  HP15_RS01335 (HP15_278) trmB 270022..270753 (+) 732 WP_014575882.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  HP15_RS01340 (HP15_279) - 270771..271304 (-) 534 WP_049784448.1 hypothetical protein -
  HP15_RS01345 (HP15_280) hemW 271304..272470 (-) 1167 WP_041644901.1 radical SAM family heme chaperone HemW -
  HP15_RS01350 (HP15_281) rdgB 272476..273075 (-) 600 WP_014575885.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  HP15_RS01355 (HP15_282) - 273082..273537 (-) 456 WP_008174175.1 DUF4426 domain-containing protein -
  HP15_RS01360 (HP15_283) metW 273540..274139 (-) 600 WP_008174176.1 methionine biosynthesis protein MetW -
  HP15_RS01365 (HP15_284) - 274136..275284 (-) 1149 WP_008174179.1 homoserine O-acetyltransferase -
  HP15_RS01370 (HP15_285) - 275412..277376 (-) 1965 WP_008174181.1 dynamin family protein -
  HP15_RS01375 (HP15_286) - 277620..278204 (-) 585 WP_008174183.1 YggT family protein -
  HP15_RS01380 (HP15_287) proC 278227..279060 (-) 834 WP_014575886.1 pyrroline-5-carboxylate reductase -
  HP15_RS01385 (HP15_288) - 279122..279826 (-) 705 WP_041644903.1 YggS family pyridoxal phosphate-dependent enzyme -
  HP15_RS01390 (HP15_289) pilT 279967..281001 (+) 1035 WP_008174189.1 type IV pilus twitching motility protein PilT Machinery gene
  HP15_RS01395 (HP15_290) pilU 281044..282165 (+) 1122 WP_014575888.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HP15_RS01400 (HP15_291) - 282405..283688 (+) 1284 WP_014575889.1 OmpP1/FadL family transporter -
  HP15_RS01405 (HP15_292) - 283720..284094 (-) 375 WP_008174194.1 hypothetical protein -
  HP15_RS01415 (HP15_293) polA 284328..287059 (-) 2732 Protein_284 DNA polymerase I -
  HP15_RS01420 (HP15_294) - 287147..287485 (+) 339 WP_008174197.1 DUF2782 domain-containing protein -
  HP15_RS01425 (HP15_295) - 287493..287972 (-) 480 WP_169702128.1 fibronectin type III domain-containing protein -
  HP15_RS01430 (HP15_296) - 288252..288674 (+) 423 WP_014575893.1 hypothetical protein -
  HP15_RS01435 (HP15_297) - 288770..289423 (-) 654 WP_014575894.1 DUF4124 domain-containing protein -
  HP15_RS01440 (HP15_298) pyrE 289427..290065 (-) 639 WP_008174208.1 orotate phosphoribosyltransferase -
  HP15_RS01445 (HP15_299) - 290147..290953 (+) 807 WP_014575895.1 exodeoxyribonuclease III -
  HP15_RS01450 (HP15_300) rph 291114..291830 (-) 717 WP_014575896.1 ribonuclease PH -
  HP15_RS01455 (HP15_301) - 291999..292865 (+) 867 WP_014575897.1 YicC/YloC family endoribonuclease -
  HP15_RS01460 (HP15_302) - 292887..293708 (-) 822 WP_014575898.1 RluA family pseudouridine synthase -
  HP15_RS01465 (HP15_303) gmk 293857..294489 (+) 633 WP_014575899.1 guanylate kinase -
  HP15_RS01470 (HP15_304) rpoZ 294576..294788 (+) 213 WP_008169593.1 DNA-directed RNA polymerase subunit omega -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41509.66 Da        Isoelectric Point: 6.5755

>NTDB_id=47819 HP15_RS01395 WP_014575888.1 281044..282165(+) (pilU) [Marinobacter adhaerens HP15]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKVLPVTKNALTPEQTREFVYGAMNDKQRAEFEETHECNFAISARGIGR
FRVSAFFQRNLCGMVLRRIEVKIPQIDDLSLPEVIKELAMTKRGLIMFVGATGTGKSTSLAAMLGHRNRNSRGHIISIED
PIEFVHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRQTMEYSVQFAETGHLCLATLHANNANQALDRI
IQFFPPEQHNQIWMDLSLNLKAIVAQQLVPTPDGKGRKAVIEVLINTPLVSDLIRKGEVHRLKELMSKSNESGMQTFDQA
LYHLYAEGSITYEDALAHADSANDLRLMIKLGADAQGADKLSSSVDKLSIQDD

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=47819 HP15_RS01395 WP_014575888.1 281044..282165(+) (pilU) [Marinobacter adhaerens HP15]
ATGGAATTCGAAAAACTGTTACGGCTGATGGTGGAGAAGGGCGGTTCGGACCTGTTTATTACAGCGGGTGTGCCGCCAAG
CATGAAGGTGAACGGGAAAGTTTTGCCGGTCACCAAGAACGCGTTGACGCCGGAGCAGACCAGGGAGTTCGTTTACGGCG
CCATGAACGACAAGCAGCGCGCCGAATTCGAGGAAACCCATGAGTGTAACTTCGCTATCAGTGCCCGTGGCATTGGCCGT
TTCCGTGTTTCTGCCTTTTTCCAGCGCAACCTGTGCGGGATGGTGCTGCGTCGGATCGAGGTAAAGATTCCGCAGATCGA
TGATCTGTCGTTGCCGGAGGTAATCAAAGAACTGGCCATGACCAAGCGTGGCCTGATCATGTTCGTGGGCGCTACCGGTA
CCGGTAAGTCTACGTCCCTGGCGGCCATGCTCGGGCATCGTAACCGCAACAGCCGTGGCCACATCATTTCCATCGAAGAC
CCAATCGAATTCGTGCACCAGCATCAGGGTTGTATCGTTACCCAGCGCGAGGTGGGCATCGATACCGAGAGCTTCGAAGT
AGCGCTGAAGAACACCCTGCGCCAGGCACCAGACGTTATTCTGATCGGTGAGGTTCGTACCCGGCAGACCATGGAATATT
CCGTGCAATTCGCCGAGACCGGTCACCTGTGTCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCCAGTTCTTCCCGCCGGAACAGCACAACCAGATCTGGATGGACCTGTCCCTGAACCTCAAGGCGATCGTGGCTCAGCA
GCTGGTGCCAACGCCAGATGGCAAGGGCCGTAAGGCGGTTATCGAGGTGCTGATCAATACCCCGCTGGTATCGGACCTGA
TCCGGAAGGGCGAGGTGCATCGGCTGAAAGAGCTGATGTCCAAATCCAACGAATCCGGTATGCAGACCTTTGATCAGGCG
CTTTATCACCTGTATGCGGAGGGTTCGATTACCTACGAGGATGCCCTGGCCCACGCAGACTCTGCCAACGACCTGCGCCT
GATGATCAAGCTGGGTGCCGACGCCCAGGGAGCCGACAAGCTCTCGTCTTCCGTCGACAAGCTTTCTATTCAAGATGACT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4PL12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

79.245

99.464

0.788
  pilU Acinetobacter baylyi ADP1

64.888

95.442

0.619
  pilU Vibrio cholerae strain A1552

57.027

99.196

0.566
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

90.885

0.386
  pilT Pseudomonas aeruginosa PAK

40.896

89.812

0.367
  pilT Acinetobacter baumannii strain A118

40.597

89.812

0.365
  pilT Acinetobacter baumannii D1279779

40.597

89.812

0.365
  pilT Pseudomonas stutzeri DSM 10701

40.597

89.812

0.365
  pilT Acinetobacter nosocomialis M2

40.299

89.812

0.362
  pilT Legionella pneumophila strain Lp02

40.299

89.812

0.362
  pilT Legionella pneumophila strain ERS1305867

40.299

89.812

0.362

Multiple sequence alignment