Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HBNCFIEN_RS10040 Genome accession   NZ_CP059576
Coordinates   2339363..2339794 (+) Length   143 a.a.
NCBI ID   WP_304599254.1    Uniprot ID   A0A7G4RHG9
Organism   Legionella sp. PC997     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2334363..2344794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBNCFIEN_RS10030 (HBNCFIEN_02026) letS 2335033..2337768 (+) 2736 WP_182393670.1 two-component system sensor histidine kinase LetS Regulator
  HBNCFIEN_RS10035 (HBNCFIEN_02027) - 2337841..2339079 (+) 1239 WP_182390959.1 6-phosphofructokinase -
  HBNCFIEN_RS10040 (HBNCFIEN_02028) pilA 2339363..2339794 (+) 432 WP_304599254.1 pilin Machinery gene
  HBNCFIEN_RS10045 (HBNCFIEN_02029) - 2339859..2340188 (+) 330 WP_182390960.1 BolA family transcriptional regulator -
  HBNCFIEN_RS10050 (HBNCFIEN_02030) - 2340289..2340627 (+) 339 WP_182390961.1 hypothetical protein -
  HBNCFIEN_RS10055 (HBNCFIEN_02031) - 2340868..2342304 (+) 1437 WP_182390962.1 APC family permease -
  HBNCFIEN_RS10060 (HBNCFIEN_02032) - 2342360..2342539 (-) 180 WP_182390963.1 Trm112 family protein -
  HBNCFIEN_RS10065 (HBNCFIEN_02033) tsaB 2342558..2343208 (-) 651 WP_182390964.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14495.73 Da        Isoelectric Point: 7.7827

>NTDB_id=470915 HBNCFIEN_RS10040 WP_304599254.1 2339363..2339794(+) (pilA) [Legionella sp. PC997]
MKEKGFTLIELMIVVAIVGILAAIAIPAYQDYVVRARVTEGLSLASSAKTTISENASTGSAALDLGWSAPPATASVASVA
VTPATGVITVTYTALANNVVLTLTPTANGNPLVAGTPPTAPITWACAVNNAANNRYVPANCRI

Nucleotide


Download         Length: 432 bp        

>NTDB_id=470915 HBNCFIEN_RS10040 WP_304599254.1 2339363..2339794(+) (pilA) [Legionella sp. PC997]
ATGAAAGAAAAAGGCTTTACTTTAATTGAATTAATGATAGTGGTAGCAATAGTTGGTATTCTTGCAGCAATTGCTATCCC
TGCATATCAGGATTACGTCGTTAGAGCTCGTGTGACAGAAGGATTAAGTCTCGCCTCCTCCGCAAAGACTACGATCTCTG
AAAATGCTTCAACTGGGTCTGCTGCCTTGGACTTAGGTTGGAGTGCACCCCCAGCTACAGCGAGTGTTGCAAGTGTTGCT
GTTACACCAGCTACTGGTGTAATCACTGTCACTTATACCGCTCTAGCTAATAACGTTGTCTTAACTTTAACTCCTACAGC
GAATGGGAACCCTCTGGTTGCCGGTACACCACCCACTGCACCAATTACCTGGGCTTGTGCGGTGAATAATGCCGCCAATA
ACCGATACGTTCCTGCAAACTGCAGAATTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)

Pilin (bacterial filament) (Pilin)

(34-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G4RHG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

50.955

100

0.559
  pilA2 Legionella pneumophila str. Paris

52.74

100

0.538
  pilA2 Legionella pneumophila strain ERS1305867

52.055

100

0.531
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40.719

100

0.476
  comP Acinetobacter baylyi ADP1

43.871

100

0.476
  pilA/pilA1 Eikenella corrodens VA1

38.509

100

0.434
  pilE Neisseria gonorrhoeae MS11

34.32

100

0.406
  pilA Pseudomonas aeruginosa PAK

33.735

100

0.392
  pilE Neisseria gonorrhoeae strain FA1090

33.742

100

0.385
  pilA Glaesserella parasuis strain SC1401

35.57

100

0.371