Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   H0H12_RS01600 Genome accession   NZ_CP059052
Coordinates   344378..345640 (+) Length   420 a.a.
NCBI ID   WP_180689133.1    Uniprot ID   A0A7D5VZV6
Organism   Pseudomonas putida strain BS3701     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 339378..350640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0H12_RS01585 (H0H12_01585) pilM 342027..342908 (+) 882 WP_180689132.1 type IV pilus biogenesis protein PilM -
  H0H12_RS01590 (H0H12_01590) - 342905..343441 (+) 537 WP_004575490.1 PilN domain-containing protein -
  H0H12_RS01595 (H0H12_01595) - 343453..344454 (+) 1002 WP_180690082.1 pilus assembly protein PilP -
  H0H12_RS01600 (H0H12_01600) pilQ 344378..345640 (+) 1263 WP_180689133.1 type IV pilus secretin PilQ Machinery gene
  H0H12_RS01605 (H0H12_01605) aroK 345645..346163 (+) 519 WP_010955625.1 shikimate kinase AroK -
  H0H12_RS01610 (H0H12_01610) aroB 346260..347357 (+) 1098 WP_180689134.1 3-dehydroquinate synthase -
  H0H12_RS01615 (H0H12_01615) - 347373..348974 (+) 1602 WP_180689135.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45681.33 Da        Isoelectric Point: 4.9022

>NTDB_id=466846 H0H12_RS01600 WP_180689133.1 344378..345640(+) (pilQ) [Pseudomonas putida strain BS3701]
MKTWMNYLLATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGSVTLRLQDVPWDQALDLVLR
SKGLSRRQEGNVLLVAPAAEFAAQSADARVSQALDAHLQPLRRELLPIHHAKAADLAQLLLAGLEGDGIPPASLSVDERT
NTLVVYLPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNKV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=466846 H0H12_RS01600 WP_180689133.1 344378..345640(+) (pilQ) [Pseudomonas putida strain BS3701]
ATGAAAACGTGGATGAACTACCTGTTGGCAACCCTGCTGTCGATCCCGCTGGCTTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGCTGAACTTCCAGGATGTTGAGGTGCGTGCGGTGCTGCAGGTGCTGGCTGACTACGCCGGCGTCAACCTGGTCG
CCAGTGATGCGGTACAAGGCAGTGTCACTTTGCGTTTGCAGGACGTGCCGTGGGATCAGGCGCTTGACCTGGTGCTGCGT
AGCAAGGGCCTGTCCCGGCGCCAGGAGGGCAATGTGCTGTTGGTGGCACCTGCAGCCGAGTTTGCCGCGCAATCAGCAGA
CGCCCGGGTGAGTCAGGCGCTGGATGCGCACCTGCAGCCATTGCGTCGGGAGCTGCTGCCCATTCACCACGCCAAGGCCG
CCGATCTGGCGCAGTTGCTGCTGGCAGGCCTGGAGGGTGACGGCATCCCTCCGGCTAGCTTGAGTGTCGATGAGCGCACC
AATACGCTGGTGGTGTACCTGCCCGCTGACCGCCTGGCCGAGTTACGCCAGCTGGTGGCGCAGTTGGATGTGCCGGTGCG
CCAGGTAGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTATGAGAAAAGCCTGGGGGTACGCTGGGGTGGGCCGC
TGTACGGCGAGAAACTGCGCCCCGGCAAGGAGCTGTTCGTCGACCTCGGTCTGGAGCGAGCCGGCAGCAGTATCGGCCTG
GGTTTGTTGCGCGGTGACGTGTTGCTGGACCTGGAGCTCAGTGCCATGGAAAAAAGCGGCAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCTGACAAGGAAACGGCGAGGATCCTCAAGGGTACCGAGGTGCCTTACCAGGAAACGAGCCAAA
GCGGCGCTACTTCGGTATCGTTTCGCGAAGCCTCGCTGTCGCTGGAAGTAACGCCACAGATCACCCCGGACAACAAGGTG
ATCATGACCGTCCGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATTGGTGGCGTCTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(125-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D5VZV6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.633

100

0.619
  comQ Acinetobacter baylyi ADP1

42.539

100

0.455
  pilQ Acinetobacter baumannii strain A118

41.215

100

0.452
  pilQ Acinetobacter baumannii D1279779

41.215

100

0.452
  pilQ Legionella pneumophila strain ERS1305867

43.029

99.048

0.426
  pilQ Legionella pneumophila strain Lp02

43.029

99.048

0.426
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.943

100

0.421
  pilQ Vibrio cholerae strain A1552

41.943

100

0.421
  pilQ Vibrio campbellii strain DS40M4

40.46

100

0.419
  pilQ Neisseria gonorrhoeae strain FA1090

35.765

100

0.362
  pilQ Neisseria meningitidis MC58

35.765

100

0.362
  pilQ Neisseria meningitidis M1080

35.765

100

0.362