Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HXS75_RS17735 Genome accession   NZ_CP058533
Coordinates   3949610..3950935 (-) Length   441 a.a.
NCBI ID   WP_032890266.1    Uniprot ID   -
Organism   Pseudomonas sivasensis strain W-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3944610..3955935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HXS75_RS17720 - 3946288..3947850 (-) 1563 WP_095186123.1 AAA family ATPase -
  HXS75_RS17725 aroB 3947861..3948961 (-) 1101 WP_032890269.1 3-dehydroquinate synthase -
  HXS75_RS17730 aroK 3949087..3949605 (-) 519 WP_010213757.1 shikimate kinase AroK -
  HXS75_RS17735 pilQ 3949610..3950935 (-) 1326 WP_032890266.1 type IV pilus secretin PilQ family protein Machinery gene
  HXS75_RS17740 - 3951005..3951976 (-) 972 WP_179179628.1 pilus assembly protein PilP -
  HXS75_RS17745 - 3951973..3952524 (-) 552 WP_095186126.1 PilN domain-containing protein -
  HXS75_RS17750 pilM 3952521..3953177 (-) 657 WP_179179629.1 type IV pilus assembly protein PilM -
  HXS75_RS17755 - 3953396..3955858 (+) 2463 WP_095186127.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47903.78 Da        Isoelectric Point: 5.0148

>NTDB_id=464163 HXS75_RS17735 WP_032890266.1 3949610..3950935(-) (pilQ) [Pseudomonas sivasensis strain W-6]
MAVAVPNRVDLIQLPPPGNAVSGAYTGEKLSLNFQDIGLRAVLQQIADVAGLNLVVSDDVQGSITLRLKHVPWDQALDLV
LQTKGLDKRIKAGVLLVAPAEELAARELLTLESRKQMADLAPLRRELLQVNYAKAADLAKLFQSVNGFEGTTDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGARWGGRLNRGNWAAGGIQKPQSDGAQTPE
NAPSSPFVDLGSLTGTSGLGIAYITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATSV
SFKEASLSLEVTPQITPDDHVIMVVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVVADKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=464163 HXS75_RS17735 WP_032890266.1 3949610..3950935(-) (pilQ) [Pseudomonas sivasensis strain W-6]
ATGGCTGTTGCTGTGCCCAATCGTGTGGATCTTATCCAGCTACCGCCTCCCGGGAATGCGGTGTCGGGCGCCTATACAGG
TGAAAAGCTGAGCCTCAACTTCCAGGACATTGGTCTGCGCGCCGTATTGCAGCAAATCGCCGATGTGGCCGGCCTCAACT
TGGTGGTCAGCGACGATGTGCAGGGCTCTATCACCCTACGCCTCAAGCATGTGCCCTGGGATCAAGCGCTGGATCTTGTG
CTGCAAACTAAAGGTTTGGATAAACGGATAAAGGCCGGAGTGCTACTGGTGGCGCCCGCCGAGGAGTTGGCCGCTCGCGA
ATTGCTCACGCTGGAATCGCGCAAGCAGATGGCCGACCTGGCGCCCCTGCGGCGTGAACTATTGCAGGTCAACTACGCTA
AGGCGGCGGACCTGGCCAAGCTGTTCCAGTCGGTGAACGGCTTTGAAGGAACGACGGATGAGCGCGGTTCGGTGGCCGTA
GATGACCGCACCAACAACATCATTGCCTACCAGACCGGCGAGCGTTTGGAAGAGCTGCGCCGGATCGTGGCGCAGTTGGA
TATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAGGCCAACGTCGACTACGACAAAAGCCTGGGCGCGCGCT
GGGGAGGTCGCCTCAATCGAGGGAATTGGGCCGCCGGAGGTATTCAGAAGCCTCAGTCGGACGGTGCCCAAACTCCTGAA
AATGCACCCAGCTCGCCGTTCGTAGATCTGGGTTCGCTTACTGGTACCTCCGGCCTGGGCATTGCGTATATCACCGACAA
CCTGCTGCTCGACCTGGAACTGACCGCCATGGAGAAAACCGGCAATGGAGAAATCGTCTCACAGCCCAAGGTGGTCACCT
CCGACAAGGAAACCGCGCGCATTCTGAAGGGCACGGAAATTCCCTACCAGGAATCCACCTCCCAAGGTGCGACGTCGGTG
TCGTTCAAGGAGGCCTCCCTTTCATTGGAGGTTACCCCGCAAATCACTCCCGATGACCATGTGATCATGGTGGTCAAAGT
CACCAAGGATGAGCCCGACTACCTCAATAAACTCAACGACGTACCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGT
TGGTCAAGGATGGAGAGACGATCGTCATTGGCGGTGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTAGTGGCGGATAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(52-99)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.588

100

0.707
  comQ Acinetobacter baylyi ADP1

42.92

100

0.44
  pilQ Acinetobacter baumannii D1279779

41.595

100

0.438
  pilQ Acinetobacter baumannii strain A118

41.595

100

0.438
  pilQ Legionella pneumophila strain Lp02

44.681

95.918

0.429
  pilQ Legionella pneumophila strain ERS1305867

44.258

94.785

0.419
  pilQ Vibrio campbellii strain DS40M4

43.396

96.145

0.417
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.301

94.785

0.41
  pilQ Vibrio cholerae strain A1552

43.301

94.785

0.41
  pilQ Neisseria gonorrhoeae strain FA1090

36.026

100

0.374
  pilQ Neisseria meningitidis MC58

35.903

100

0.37
  pilQ Neisseria meningitidis M1080

35.903

100

0.37