NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	JHW37_RS24260	Genome accession	NZ_CP068238
Coordinates	5175088..5175558 (-)	Length	156 a.a.
NCBI ID	WP_003152762.1	Uniprot ID	-
Organism	Pseudomonas aeruginosa strain A39-1
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5170088..5180558

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JHW37_RS24230 (JHW37_24230)	-	5170117..5171019 (+)	903	WP_079741177.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
JHW37_RS24235 (JHW37_24235)	-	5171008..5171655 (-)	648	WP_201268020.1	hypothetical protein	-
JHW37_RS30330 (JHW37_24240)	-	5171783..5172025 (+)	243	WP_079741178.1	winged helix-turn-helix domain-containing protein	-
JHW37_RS24250 (JHW37_24250)	tfpZ	5172372..5173106 (-)	735	WP_124179916.1	TfpX/TfpZ family type IV pilin accessory protein	-
JHW37_RS24255 (JHW37_24255)	-	5173106..5175025 (-)	1920	WP_124179915.1	hypothetical protein	-
JHW37_RS24260 (JHW37_24260)	pilA	5175088..5175558 (-)	471	WP_003152762.1	pilin	Machinery gene
JHW37_RS24265 (JHW37_24265)	pilB	5175781..5177484 (+)	1704	WP_033998207.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JHW37_RS24270 (JHW37_24270)	pilC	5177488..5178705 (+)	1218	WP_012077296.1	type II secretion system F family protein	Machinery gene
JHW37_RS24275 (JHW37_24275)	pilD	5178708..5179580 (+)	873	WP_053817760.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
JHW37_RS24280 (JHW37_24280)	coaE	5179577..5180188 (+)	612	WP_003152767.1	dephospho-CoA kinase	-
JHW37_RS24285 (JHW37_24285)	yacG	5180185..5180385 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 156 a.a. Molecular weight: 15836.16 Da Isoelectric Point: 8.4426

                                >NTDB_id=459254 JHW37_RS24260 WP_003152762.1 5175088..5175558(-) (pilA) [Pseudomonas aeruginosa strain A39-1]

MKAQKGFTLIELMIVVAIIGILAAVAIPAYQDYITRGQVTEAVSLGGGLKSPLAEYGADKNAWPTALVAPTVTPSANQLN

ATLIGKYSTVTPSIGGSYPSGVITVTMTTGKAANSVLTLTTTNGGSSWACGNAKVDDAQGSGTTIDAKYLPNACKP

Nucleotide

Download Length: 471 bp

                                >NTDB_id=459254 JHW37_RS24260 WP_003152762.1 5175088..5175558(-) (pilA) [Pseudomonas aeruginosa strain A39-1]

ATGAAAGCGCAAAAAGGCTTTACCCTGATCGAACTGATGATCGTGGTGGCGATCATCGGCATTCTGGCTGCCGTCGCTAT

CCCGGCTTATCAGGACTACATCACCCGCGGTCAGGTCACCGAGGCCGTATCCCTGGGCGGTGGTCTGAAGTCCCCGCTGG

CCGAGTATGGCGCCGACAAGAACGCTTGGCCGACTGCTTTGGTTGCTCCGACCGTGACCCCTTCCGCGAACCAACTTAAT

GCAACTCTGATCGGTAAATACAGCACCGTTACCCCCTCCATTGGTGGTAGCTACCCGTCGGGTGTCATTACCGTAACCAT

GACGACTGGCAAGGCAGCTAACAGCGTTCTGACTCTGACCACCACAAATGGTGGTTCGAGCTGGGCCTGTGGCAACGCCA

AGGTAGATGACGCTCAAGGTTCCGGTACCACCATCGATGCTAAATATCTGCCGAACGCCTGCAAGCCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-155)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Ralstonia pseudosolanacearum GMI1000	42.442	100	0.468
pilA/pilA1	Eikenella corrodens VA1	42.515	100	0.455
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	34.359	100	0.429
comP	Acinetobacter baylyi ADP1	40.361	100	0.429
pilE	Neisseria gonorrhoeae MS11	40.244	100	0.423
pilA2	Legionella pneumophila str. Paris	40.625	100	0.417
pilA2	Legionella pneumophila strain ERS1305867	40.625	100	0.417
pilE	Neisseria gonorrhoeae strain FA1090	39.024	100	0.41
pilA/pilAI	Pseudomonas stutzeri DSM 10701	38.415	100	0.404
pilA	Pseudomonas aeruginosa PAK	36.047	100	0.397
pilA	Acinetobacter baumannii strain A118	38.71	99.359	0.385

Detailed information NTG0459254