Detailed information NTG0451746

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: I9X26_RS27625
• Genome accession: NZ_CP065865
• Coordinates: 5808312..5808785 (-)
• Length: 157 a.a.
• NCBI ID: WP_015503553.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain TJ2019-022
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5803312..5813785

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I9X26_RS27590 (I9X26_27590)	-	5803755..5804417 (+)	663	WP_033992423.1	DUF3218 family protein	-
I9X26_RS27595 (I9X26_27595)	fliP	5804697..5805452 (+)	756	WP_031285507.1	flagellar type III secretion system pore protein FliP	-
I9X26_RS27600 (I9X26_27600)	fliQ	5805497..5805766 (+)	270	WP_023435365.1	flagellar biosynthesis protein FliQ	-
I9X26_RS27605 (I9X26_27605)	fliR	5805766..5806548 (+)	783	Protein_5485	flagellar biosynthetic protein FliR	-
I9X26_RS27610 (I9X26_27610)	-	5806551..5806810 (+)	260	Protein_5486	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
I9X26_RS27615 (I9X26_27615)	-	5807839..5808081 (+)	243	WP_023082027.1	hypothetical protein	-
I9X26_RS27625 (I9X26_27625)	pilE	5808312..5808785 (-)	474	WP_015503553.1	pilin	Machinery gene
I9X26_RS27630 (I9X26_27630)	pilB	5809016..5810716 (+)	1701	WP_003120945.1	type IV-A pilus assembly ATPase PilB	Machinery gene
I9X26_RS27635 (I9X26_27635)	-	5810720..5811938 (+)	1219	Protein_5490	type II secretion system F family protein	-
I9X26_RS27640 (I9X26_27640)	pilD	5811941..5812813 (+)	873	WP_003116251.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
I9X26_RS27645 (I9X26_27645)	coaE	5812810..5813421 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
I9X26_RS27650 (I9X26_27650)	yacG	5813418..5813618 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 157 a.a.
• Molecular weight: 16090.45 Da
• Isoelectric Point: 8.4684

>NTDB_id=451746 I9X26_RS27625 WP_015503553.1 5808312..5808785(-) (pilE) [Pseudomonas aeruginosa strain TJ2019-022]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKASDVATPLRGKTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide

• Length: 474 bp

>NTDB_id=451746 I9X26_RS27625 WP_015503553.1 5808312..5808785(-) (pilE) [Pseudomonas aeruginosa strain TJ2019-022]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGCCTCTGATGTGGCTACTCCTCTGAGGGGGAAAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA

Domains

Predicted by InterProScan.

• Pilin (bacterial filament) (Pilin) (36-150)

• Prokaryotic N-terminal methylation motif (N_methyl) (4-27)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Neisseria gonorrhoeae strain FA1090	42.197	100	0.465
comP	Acinetobacter baylyi ADP1	47.059	97.452	0.459
pilE	Neisseria gonorrhoeae MS11	42.515	100	0.452
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	41.176	100	0.446
pilA/pilA1	Eikenella corrodens VA1	41.975	100	0.433
pilA2	Legionella pneumophila str. Paris	45.578	93.631	0.427
pilA2	Legionella pneumophila strain ERS1305867	44.218	93.631	0.414
pilA	Ralstonia pseudosolanacearum GMI1000	39.394	100	0.414
pilA/pilAI	Pseudomonas stutzeri DSM 10701	43.333	95.541	0.414
pilA	Pseudomonas aeruginosa PAK	41.722	96.178	0.401
pilA	Acinetobacter nosocomialis M2	55.357	71.338	0.395