Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   HRE60_RS08970 Genome accession   NZ_CP054153
Coordinates   1970174..1971115 (-) Length   313 a.a.
NCBI ID   WP_181670753.1    Uniprot ID   -
Organism   Streptococcus salivarius strain DB-B5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1965174..1976115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HRE60_RS08930 (HRE60_08930) - 1965249..1966442 (-) 1194 WP_002885831.1 acetate kinase -
  HRE60_RS08935 (HRE60_08935) comYH 1966499..1967455 (-) 957 WP_002885753.1 class I SAM-dependent methyltransferase Machinery gene
  HRE60_RS08940 (HRE60_08940) comGG 1967500..1967817 (-) 318 WP_037605448.1 competence type IV pilus minor pilin ComGG -
  HRE60_RS08945 (HRE60_08945) comYF 1967795..1968232 (-) 438 WP_181670751.1 competence type IV pilus minor pilin ComGF Machinery gene
  HRE60_RS08950 (HRE60_08950) comGE 1968219..1968449 (-) 231 WP_021144685.1 competence type IV pilus minor pilin ComGE -
  HRE60_RS08955 (HRE60_08955) comYD 1968481..1968909 (-) 429 WP_014632541.1 competence type IV pilus minor pilin ComGD Machinery gene
  HRE60_RS08960 (HRE60_08960) comYC 1968869..1969183 (-) 315 WP_037611125.1 competence type IV pilus major pilin ComGC Machinery gene
  HRE60_RS08965 (HRE60_08965) comYB 1969192..1970292 (-) 1101 WP_181670752.1 competence type IV pilus assembly protein ComGB Machinery gene
  HRE60_RS08970 (HRE60_08970) comYA 1970174..1971115 (-) 942 WP_181670753.1 competence type IV pilus ATPase ComGA Machinery gene
  HRE60_RS08975 (HRE60_08975) - 1971195..1971557 (-) 363 WP_002887019.1 DUF1033 family protein -
  HRE60_RS08980 (HRE60_08980) rpoC 1971720..1975358 (-) 3639 WP_013991269.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35310.34 Da        Isoelectric Point: 5.7041

>NTDB_id=451198 HRE60_RS08970 WP_181670753.1 1970174..1971115(-) (comYA) [Streptococcus salivarius strain DB-B5]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGQRVSLRLSTVGDYRGLESLVIRVLHSERRELVYWNQGIQPIMDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDNLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSVAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFASENFQSHSSTNWNQQLEGLVKQGYLTEGDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=451198 HRE60_RS08970 WP_181670753.1 1970174..1971115(-) (comYA) [Streptococcus salivarius strain DB-B5]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATTTATGTCATTCCACGTCA
GGATAATTACGAGCTCTATATGCGAGTTGGTCAGGAGAGGAGATTAATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTAGGTTCCTGTGACTATGAT
TGTGGTGATGGTCAAAGGGTTTCTCTGCGTTTGTCTACCGTTGGGGATTATCGCGGCTTAGAAAGTTTAGTTATTCGTGT
TCTACATTCTGAACGTCGAGAATTAGTGTACTGGAATCAAGGAATCCAGCCTATTATGGATGCTTTGGATTATCGTGGCT
TATATCTCTTTGCAGGTCCCGTTGGTTCTGGAAAGACTACGCTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTGATTTCGATTGAGGACCCTGTGGAAATTAAACAGGATAATGTCTTGCAACTCCAGGTGAATCAAGCAATTGACAT
GACCTATGATAATTTGATTAAGCTATCATTACGTCACCGCCCGGATGTTTTGATTATTGGAGAAATTCGAGATAAGGAGA
CTGCTCGAGCGGTTATTAGAGCTAGTCTGACGGGGGTGACTGTTCTTTCAACTATTCACGCAAAGAGTGTGGCAGGTGTT
TATGAGCGTCTTCTGGACCTCGGTGTAGATAAGTCTGAGTTGGATAATGCCCTTCAAGGGATTGCCTACATGCGTCTGAT
TAAGGGAGGAGGTGTGATTGATTTTGCCAGTGAAAATTTCCAAAGCCATTCGTCAACCAACTGGAACCAGCAGTTGGAAG
GCTTGGTTAAACAAGGATATCTCACTGAAGGGGATATCCAAGGGGAAAAAATTAAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

65.176

100

0.652
  comYA Streptococcus mutans UA140

65.176

100

0.652
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.595

99.361

0.652
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.63

99.361

0.642
  comYA Streptococcus gordonii str. Challis substr. CH1

62.939

100

0.629
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.897

99.681

0.607
  comGA Lactococcus lactis subsp. cremoris KW2

51.447

99.361

0.511
  comGA Latilactobacillus sakei subsp. sakei 23K

41.697

86.581

0.361