Detailed information NTG0451194

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: HRE60_RS08945
• Genome accession: NZ_CP054153
• Coordinates: 1967795..1968232 (-)
• Length: 145 a.a.
• NCBI ID: WP_181670751.1
• Uniprot ID: A0A7L6WLZ7
• Organism: Streptococcus salivarius strain DB-B5
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1962795..1973232

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HRE60_RS08910 (HRE60_08910)	-	1962833..1963495 (-)	663	WP_002885821.1	type II CAAX endopeptidase family protein	-
HRE60_RS08915 (HRE60_08915)	-	1963608..1964054 (-)	447	WP_181670750.1	CAAX protease	-
HRE60_RS08920 (HRE60_08920)	-	1964132..1964788 (-)	657	WP_073687812.1	type II CAAX endopeptidase family protein	-
HRE60_RS08925 (HRE60_08925)	-	1964800..1964997 (-)	198	WP_084870139.1	helix-turn-helix transcriptional regulator	-
HRE60_RS08930 (HRE60_08930)	-	1965249..1966442 (-)	1194	WP_002885831.1	acetate kinase	-
HRE60_RS08935 (HRE60_08935)	comYH	1966499..1967455 (-)	957	WP_002885753.1	class I SAM-dependent methyltransferase	Machinery gene
HRE60_RS08940 (HRE60_08940)	comGG	1967500..1967817 (-)	318	WP_037605448.1	competence type IV pilus minor pilin ComGG	-
HRE60_RS08945 (HRE60_08945)	comYF	1967795..1968232 (-)	438	WP_181670751.1	competence type IV pilus minor pilin ComGF	Machinery gene
HRE60_RS08950 (HRE60_08950)	comGE	1968219..1968449 (-)	231	WP_021144685.1	competence type IV pilus minor pilin ComGE	-
HRE60_RS08955 (HRE60_08955)	comYD	1968481..1968909 (-)	429	WP_014632541.1	competence type IV pilus minor pilin ComGD	Machinery gene
HRE60_RS08960 (HRE60_08960)	comYC	1968869..1969183 (-)	315	WP_037611125.1	competence type IV pilus major pilin ComGC	Machinery gene
HRE60_RS08965 (HRE60_08965)	comYB	1969192..1970292 (-)	1101	WP_181670752.1	competence type IV pilus assembly protein ComGB	Machinery gene
HRE60_RS08970 (HRE60_08970)	comYA	1970174..1971115 (-)	942	WP_181670753.1	competence type IV pilus ATPase ComGA	Machinery gene
HRE60_RS08975 (HRE60_08975)	-	1971195..1971557 (-)	363	WP_002887019.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16154.55 Da
• Isoelectric Point: 8.4658

>NTDB_id=451194 HRE60_RS08945 WP_181670751.1 1967795..1968232(-) (comYF) [Streptococcus salivarius strain DB-B5]
MSLNSKVRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQAKQQENQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSATDFRKTNADGRGYQPMLFNVKASTISQNNQIVTIQVTLKNGLQRSFVYAFEKTG

Nucleotide

• Length: 438 bp

>NTDB_id=451194 HRE60_RS08945 WP_181670751.1 1967795..1968232(-) (comYF) [Streptococcus salivarius strain DB-B5]
ATGTCTCTCAATAGCAAGGTTCGTGCTTTTACCCTCTTGGAATGTCTAGTAGCTCTATTGGTGATTGCTGGTTCAGTTCA
AGTTTATCAAGGTCTGACCACAGTATTGGTGAGCAATGTTAAGCAGGCCAAGCAACAAGAAAACCAAGACTGGCTCCTAT
TTGTCCAACAGATGGAGGCAGAATTAGAAGGTTGTCAGTTGGTAAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACCTGGCTTTTGGCCTTTCAAGTGCTACTGATTTTCGAAAAACAAATGCTGATGGTCGAGGCTATCAACCAAT
GCTTTTCAATGTAAAAGCTAGTACAATTAGCCAAAATAACCAGATAGTCACCATCCAAGTGACTCTTAAAAATGGGCTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A7L6WLZ7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	58.156	97.241	0.566
comYF	Streptococcus mutans UA159	57.447	97.241	0.559
comGF/cglF	Streptococcus pneumoniae Rx1	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae D39	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae R6	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae TIGR4	50	97.931	0.49
comGF/cglF	Streptococcus mitis NCTC 12261	47.222	99.31	0.469
comGF/cglF	Streptococcus mitis SK321	47.222	99.31	0.469
comGF	Lactococcus lactis subsp. cremoris KW2	45.324	95.862	0.434