Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   HRE60_RS08965 Genome accession   NZ_CP054153
Coordinates   1969192..1970292 (-) Length   366 a.a.
NCBI ID   WP_181670752.1    Uniprot ID   A0A7L6WPL3
Organism   Streptococcus salivarius strain DB-B5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1964192..1975292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HRE60_RS08925 (HRE60_08925) - 1964800..1964997 (-) 198 WP_084870139.1 helix-turn-helix transcriptional regulator -
  HRE60_RS08930 (HRE60_08930) - 1965249..1966442 (-) 1194 WP_002885831.1 acetate kinase -
  HRE60_RS08935 (HRE60_08935) comYH 1966499..1967455 (-) 957 WP_002885753.1 class I SAM-dependent methyltransferase Machinery gene
  HRE60_RS08940 (HRE60_08940) comGG 1967500..1967817 (-) 318 WP_037605448.1 competence type IV pilus minor pilin ComGG -
  HRE60_RS08945 (HRE60_08945) comYF 1967795..1968232 (-) 438 WP_181670751.1 competence type IV pilus minor pilin ComGF Machinery gene
  HRE60_RS08950 (HRE60_08950) comGE 1968219..1968449 (-) 231 WP_021144685.1 competence type IV pilus minor pilin ComGE -
  HRE60_RS08955 (HRE60_08955) comYD 1968481..1968909 (-) 429 WP_014632541.1 competence type IV pilus minor pilin ComGD Machinery gene
  HRE60_RS08960 (HRE60_08960) comYC 1968869..1969183 (-) 315 WP_037611125.1 competence type IV pilus major pilin ComGC Machinery gene
  HRE60_RS08965 (HRE60_08965) comYB 1969192..1970292 (-) 1101 WP_181670752.1 competence type IV pilus assembly protein ComGB Machinery gene
  HRE60_RS08970 (HRE60_08970) comYA 1970174..1971115 (-) 942 WP_181670753.1 competence type IV pilus ATPase ComGA Machinery gene
  HRE60_RS08975 (HRE60_08975) - 1971195..1971557 (-) 363 WP_002887019.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 41718.87 Da        Isoelectric Point: 10.0886

>NTDB_id=451197 HRE60_RS08965 WP_181670752.1 1969192..1970292(-) (comYB) [Streptococcus salivarius strain DB-B5]
MPVKISKAIRQPTGTSSWKAWLNKDISLKGISKGKKLKINQQLKVIQLFKQLLKAGFTLTEIVDFLERSHLLKESSLSLM
KASLMRGDRLDQMFATVGFSDNIVTQIALADKHGNLLGSLTKIETYMLRMTKVRKKLMEVATYPILLLGFLILIMLGLKN
YLLPQLLEGDGKENWAVQLVQIFPQLFFVTLCGLLVLSLILYLWVKRQPALVFYRRMAKIPFIGQTVRLYTTAYYAREWG
NLLGQGIDLLDLVALMKEQKLKLFRELGADLEEALMLGQSFPDRVASHPFFTKELSLIIAYGEANARLGYELEVYAEEVW
QAFFNRLNKATTFVQPLVFVIVAVVIVMIYAAMLLPMYQNMEGMMS

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=451197 HRE60_RS08965 WP_181670752.1 1969192..1970292(-) (comYB) [Streptococcus salivarius strain DB-B5]
TTGCCAGTGAAAATTTCCAAAGCCATTCGTCAACCAACTGGAACCAGCAGTTGGAAGGCTTGGTTAAACAAGGATATCTC
ACTGAAGGGGATATCCAAGGGGAAAAAATTAAAGATTAATCAGCAACTCAAGGTTATCCAGCTTTTCAAACAACTTTTAA
AGGCAGGGTTTACCTTGACTGAAATCGTAGACTTTTTGGAGCGAAGTCACTTGTTGAAAGAATCATCCTTGTCTCTTATG
AAGGCGAGCTTAATGCGAGGCGACAGGTTGGACCAGATGTTTGCAACAGTGGGCTTTTCGGACAATATTGTTACTCAGAT
TGCTCTTGCTGATAAGCACGGTAATCTTCTAGGGAGTTTAACCAAGATTGAAACCTACATGCTTCGTATGACCAAGGTTC
GTAAGAAACTCATGGAGGTAGCGACCTACCCTATTCTACTTCTGGGTTTCCTGATTCTGATTATGTTGGGACTCAAAAAT
TATCTTCTGCCTCAATTGTTAGAGGGGGATGGTAAGGAGAATTGGGCTGTACAGTTGGTTCAGATTTTTCCCCAGCTCTT
TTTTGTGACTTTGTGCGGACTTCTTGTATTAAGCTTAATTCTCTATCTATGGGTGAAACGCCAGCCAGCCCTTGTCTTTT
ATCGCCGAATGGCAAAAATCCCTTTTATAGGCCAAACTGTCAGGCTATATACGACCGCCTATTATGCTAGGGAATGGGGA
AATCTCTTAGGGCAAGGTATTGACTTGCTAGACTTAGTTGCTCTAATGAAAGAGCAAAAGTTAAAGCTTTTTCGTGAGCT
GGGGGCTGATTTAGAAGAAGCCTTGATGCTAGGACAGAGTTTCCCTGACCGTGTTGCTAGTCACCCTTTCTTCACTAAGG
AACTATCCTTAATTATTGCTTATGGAGAGGCTAATGCTAGGTTGGGCTATGAGTTAGAAGTCTATGCTGAAGAGGTTTGG
CAGGCTTTCTTTAACCGTCTTAATAAGGCAACAACCTTTGTGCAACCCCTCGTTTTTGTTATTGTTGCTGTCGTGATTGT
AATGATTTATGCAGCTATGCTATTACCAATGTATCAAAATATGGAAGGAATGATGTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(236-356)

Type II secretion system (T2SS), protein F (T2SSF)

(51-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7L6WPL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

54.81

93.716

0.514
  comYB Streptococcus mutans UA159

54.81

93.716

0.514
  comYB Streptococcus gordonii str. Challis substr. CH1

51.312

93.716

0.481
  comGB/cglB Streptococcus mitis NCTC 12261

49.558

92.623

0.459
  comGB/cglB Streptococcus mitis SK321

48.817

92.35

0.451
  comGB/cglB Streptococcus pneumoniae Rx1

48.521

92.35

0.448
  comGB/cglB Streptococcus pneumoniae D39

48.521

92.35

0.448
  comGB/cglB Streptococcus pneumoniae R6

48.521

92.35

0.448
  comGB/cglB Streptococcus pneumoniae TIGR4

48.521

92.35

0.448
  comGB Lactococcus lactis subsp. cremoris KW2

44.073

89.891

0.396