Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HPY07_RS02340 Genome accession   NZ_CP053806
Coordinates   489538..489990 (-) Length   150 a.a.
NCBI ID   WP_111673800.1    Uniprot ID   -
Organism   Vibrio cholerae strain SP6G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 484538..494990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY07_RS02320 (HPY07_02320) coaE 485040..485648 (-) 609 WP_057554975.1 dephospho-CoA kinase -
  HPY07_RS02325 (HPY07_02325) pilD 485645..486520 (-) 876 WP_071170712.1 A24 family peptidase Machinery gene
  HPY07_RS02330 (HPY07_02330) pilC 486578..487804 (-) 1227 WP_114774363.1 type II secretion system F family protein Machinery gene
  HPY07_RS02335 (HPY07_02335) pilB 487847..489535 (-) 1689 WP_175241855.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY07_RS02340 (HPY07_02340) pilA 489538..489990 (-) 453 WP_111673800.1 pilin Machinery gene
  HPY07_RS02345 (HPY07_02345) nadC 490235..491125 (-) 891 WP_175241856.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY07_RS02350 (HPY07_02350) ampD 491423..491968 (+) 546 WP_001959072.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY07_RS02355 (HPY07_02355) - 492105..493493 (+) 1389 WP_043881226.1 IS4-like element ISVa18 family transposase -
  HPY07_RS02360 (HPY07_02360) fldB 493539..494057 (-) 519 WP_000690112.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15485.57 Da        Isoelectric Point: 9.3939

>NTDB_id=447598 HPY07_RS02340 WP_111673800.1 489538..489990(-) (pilA) [Vibrio cholerae strain SP6G]
MKAYKNKLQKGFTLIELMIVVAVIGVLAAIAVPQYQKYVAKSEAASALATLTGLKTNAEAYTVETGSFPTDTQQAQLGTP
SSAMGTIAYANTGSSGAAGTITFTFSGTVSPSLKSSAIRLKRDDSGNWSCESNKDATSGIIPKGCNSGTF

Nucleotide


Download         Length: 453 bp        

>NTDB_id=447598 HPY07_RS02340 WP_111673800.1 489538..489990(-) (pilA) [Vibrio cholerae strain SP6G]
ATGAAAGCGTATAAAAATAAACTACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCAGTGATTGGTGTATT
GGCAGCGATTGCGGTACCGCAATACCAGAAATACGTAGCGAAAAGTGAAGCAGCTTCTGCATTAGCTACGTTAACTGGCT
TAAAAACCAACGCAGAGGCTTATACCGTTGAAACAGGATCTTTTCCCACAGATACTCAACAAGCTCAACTAGGTACACCA
TCATCTGCAATGGGTACTATTGCATATGCTAATACCGGTAGCAGTGGTGCTGCAGGAACAATTACATTTACCTTTAGTGG
TACGGTTAGCCCTAGTTTGAAAAGCAGCGCTATCCGTTTAAAACGAGATGATTCAGGAAACTGGTCATGTGAATCCAACA
AAGATGCAACTAGTGGGATAATACCTAAAGGATGTAATTCTGGCACTTTCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

53.896

100

0.553
  pilA Vibrio cholerae strain A1552

53.896

100

0.553
  pilA Vibrio cholerae C6706

53.896

100

0.553
  pilA Pseudomonas aeruginosa PAK

47.682

100

0.48
  pilA Vibrio parahaemolyticus RIMD 2210633

46.212

88

0.407
  pilA Acinetobacter baumannii strain A118

42.254

94.667

0.4
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.222

90

0.38
  pilA Acinetobacter nosocomialis M2

39.706

90.667

0.36