Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HPY07_RS02335 Genome accession   NZ_CP053806
Coordinates   487847..489535 (-) Length   562 a.a.
NCBI ID   WP_175241855.1    Uniprot ID   -
Organism   Vibrio cholerae strain SP6G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 482847..494535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY07_RS02310 (HPY07_02310) yacG 483913..484110 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  HPY07_RS02315 (HPY07_02315) zapD 484268..485008 (-) 741 WP_000207194.1 cell division protein ZapD -
  HPY07_RS02320 (HPY07_02320) coaE 485040..485648 (-) 609 WP_057554975.1 dephospho-CoA kinase -
  HPY07_RS02325 (HPY07_02325) pilD 485645..486520 (-) 876 WP_071170712.1 A24 family peptidase Machinery gene
  HPY07_RS02330 (HPY07_02330) pilC 486578..487804 (-) 1227 WP_114774363.1 type II secretion system F family protein Machinery gene
  HPY07_RS02335 (HPY07_02335) pilB 487847..489535 (-) 1689 WP_175241855.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY07_RS02340 (HPY07_02340) pilA 489538..489990 (-) 453 WP_111673800.1 pilin Machinery gene
  HPY07_RS02345 (HPY07_02345) nadC 490235..491125 (-) 891 WP_175241856.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY07_RS02350 (HPY07_02350) ampD 491423..491968 (+) 546 WP_001959072.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY07_RS02355 (HPY07_02355) - 492105..493493 (+) 1389 WP_043881226.1 IS4-like element ISVa18 family transposase -
  HPY07_RS02360 (HPY07_02360) fldB 493539..494057 (-) 519 WP_000690112.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62168.21 Da        Isoelectric Point: 6.0856

>NTDB_id=447597 HPY07_RS02335 WP_175241855.1 487847..489535(-) (pilB) [Vibrio cholerae strain SP6G]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGFFHPQELTEQLSHIFGLPETDLSRYDYANLCQQLGLRE
LITRYDTLPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKITVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=447597 HPY07_RS02335 WP_175241855.1 487847..489535(-) (pilB) [Vibrio cholerae strain SP6G]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCAACGCAAGAACAAGCGGTGGTTACCCAGGT
TAGCGCTTCGGGAACTTCAGTGCCCGAGGCGCTGCTTGGTTTAGGTTTTTTTCACCCCCAAGAACTGACCGAGCAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGTTACGACTACGCCAACCTGTGCCAACAGCTCGGGCTGCGTGAA
CTGATTACCCGCTACGATACCTTGCCGATTGCCAAGCAAGGCAATTTATTGCTGCTGGCGGTCTCAGACCCGACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCTACAGGTTTACAAGTTGAACTGGCACTGGCTGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCAGATCTATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAACTGCAATCGATCGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCATATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGGATCTTGGTGGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCTGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGTTTGCCGCAAGACGGGCGAATTAAACTGCGCCTAAGCCGCGATACCGCGAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGAGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTACCTCAATGCCCTCAAAAGACCGCAAGGGATGATT
TTGATGACCGGCCCAACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTCAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTAGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAACCGAAAATCGGCTTTG
GCTTCGCCGAAGCGCTACGCTCATTTCTACGTCAAGACCCTGATGTGGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCAGAAATCGCGGTAAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACCCTGCATACCAACTCGGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAACCTCGCGTCATCACTCAGTTTAATCATCGCCCAGCGTCTTGCGC
GCCGCTTGTGTAAACATTGCAAAATCACGGTGCGCCCTTCTGCCCTATTGCAAAGTCAATTTGCGTTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGATATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAACTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

98.932

100

0.989
  pilB Vibrio parahaemolyticus RIMD 2210633

73.843

100

0.738
  pilB Vibrio campbellii strain DS40M4

73.665

100

0.737
  pilB Legionella pneumophila strain ERS1305867

52.642

90.925

0.479
  pilB Acinetobacter baylyi ADP1

50.385

92.527

0.466
  pilB Acinetobacter baumannii D1279779

50.192

92.527

0.464
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

36.5

100

0.39
  pilF Thermus thermophilus HB27

39.668

96.441

0.383
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37