Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FX982_RS09465 Genome accession   NZ_CP053746
Coordinates   2072721..2074805 (+) Length   694 a.a.
NCBI ID   WP_172610442.1    Uniprot ID   -
Organism   Pseudomonas graminis strain PgKB30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2067721..2079805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX982_RS09445 (FX982_01893) pilM 2069866..2070930 (+) 1065 WP_074890782.1 pilus assembly protein PilM Machinery gene
  FX982_RS09450 (FX982_01894) - 2070930..2071499 (+) 570 WP_122533931.1 PilN domain-containing protein -
  FX982_RS09455 (FX982_01895) pilO 2071496..2072143 (+) 648 WP_172610441.1 type 4a pilus biogenesis protein PilO -
  FX982_RS09460 (FX982_01896) - 2072140..2072667 (+) 528 WP_065986622.1 pilus assembly protein PilP -
  FX982_RS09465 (FX982_01897) pilQ 2072721..2074805 (+) 2085 WP_172610442.1 type IV pilus secretin PilQ Machinery gene
  FX982_RS09470 (FX982_01898) aroK 2074810..2075328 (+) 519 WP_065986620.1 shikimate kinase AroK -
  FX982_RS09475 (FX982_01899) aroB 2075459..2076562 (+) 1104 WP_172610443.1 3-dehydroquinate synthase -
  FX982_RS09480 (FX982_01900) - 2076573..2078198 (+) 1626 WP_172610444.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 694 a.a.        Molecular weight: 74494.48 Da        Isoelectric Point: 5.1110

>NTDB_id=446824 FX982_RS09465 WP_172610442.1 2072721..2074805(+) (pilQ) [Pseudomonas graminis strain PgKB30]
MNRILSIIGLSLGMAMLSPVVQAANLKTLDVAALPGDRVELKLSFDGPVAAPRGYTTEQPARIALDLPGVTSQLAIKSRD
LGSGNAHSVVVVEAKDRTRVIINLTTLAPYSSRVDGNNLFVVVGQGAAAAQGSQPSTATGAARVSVPSGPAAQSKPVARS
YAPGPRSVKNVDFQRGELGEGNVIIELSDTGIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVNSSATGDK
ASISIEPSGAFDYSAYQTDNKLTISVRPLTNEDQERRNSEKLVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQ
GGITLRLQNVPWDQALDLVLKTKGLDKRKIGNVLLVGPADEIAARERQELESLKQISELAPLRRELLQVNYAKAADIAKL
FQSVTSAESKTDERGSITVDERTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKALGVRWGGSSNRG
NFTTGGSGTVTGAAATNGPYVDMGVTDATSSIGLGFLTNNTILDLELTAMEKSGNGEVVSQPKVVTSDKETAKILKGTEV
PYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYVNTVLGVPPIKKNEVNAKVLVADGETIVIGGVF
SNTQSKVVDKVPFLGDVPYVGRLFRRDVVSESKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2085 bp        

>NTDB_id=446824 FX982_RS09465 WP_172610442.1 2072721..2074805(+) (pilQ) [Pseudomonas graminis strain PgKB30]
ATGAACAGGATTCTTTCGATTATCGGCCTGTCGCTAGGGATGGCGATGCTTTCACCGGTTGTCCAGGCGGCTAACCTCAA
GACGCTGGATGTCGCCGCATTGCCGGGGGATCGAGTCGAATTGAAACTGTCCTTCGACGGTCCGGTGGCGGCGCCTCGCG
GCTACACCACCGAGCAGCCGGCGCGCATCGCGCTGGACTTGCCGGGCGTGACCAGCCAGTTGGCGATCAAGAGCCGTGAC
CTGGGTTCAGGCAATGCCCACAGCGTAGTGGTCGTCGAAGCCAAGGACCGCACGCGCGTGATCATCAACCTCACCACACT
CGCGCCGTACAGCTCGCGTGTCGACGGCAACAACCTGTTCGTGGTCGTGGGGCAGGGCGCTGCCGCGGCCCAGGGCTCGC
AGCCGAGCACTGCCACAGGCGCAGCGCGAGTGAGTGTGCCGAGCGGACCTGCCGCGCAATCGAAACCCGTTGCCCGCTCG
TATGCGCCGGGCCCACGGTCGGTAAAAAACGTCGACTTCCAGCGTGGAGAGTTGGGTGAAGGCAACGTCATCATTGAATT
GAGCGACACCGGCATTGCGCCCGATATTCAGGAGCAGGGGGGCAAGATCCGCGTTGATTTCGCCAAAACGCAACTGCCTG
AACCGCTGCGCGTGCGTCTGGATGTGAAGGATTTCGCGACCCCGGTGCAGTTCGTCAATTCCAGCGCCACAGGTGACAAG
GCCAGCATCTCCATCGAGCCGTCCGGCGCGTTTGATTACTCCGCCTACCAGACCGACAACAAGCTGACCATTAGCGTGCG
TCCCCTGACCAACGAAGATCAGGAGCGCCGCAACTCGGAGAAACTGGTCTACACCGGCGAGAAACTGTCGCTGAATTTCC
AGGACATCGACGTGCGTTCGGTGCTTCAACTGATCGCCGACTTCACCAACCTCAATCTCGTGGCGAGCGACACCGTTCAA
GGCGGCATCACCCTGCGCCTGCAGAACGTGCCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGCCTGGATAA
GCGCAAGATCGGTAACGTTTTGCTGGTCGGTCCTGCTGATGAGATCGCCGCTCGCGAGCGCCAGGAGCTGGAATCCCTGA
AGCAGATTTCCGAGCTTGCCCCCCTGCGTCGCGAGTTGCTGCAAGTGAACTACGCCAAGGCCGCCGACATCGCCAAGCTG
TTCCAGTCGGTGACCAGCGCTGAATCGAAGACCGACGAGCGCGGTTCGATCACCGTCGATGAGCGCACCAACAACATCAT
TGCCTACCAGACTCAGGATCGTCTCGACGAGTTGCGCCGCATCGTGTCGCAGCTGGACATCCCGGTGCGCCAGGTGATGA
TCGAGGCCCGTATCGTCGAAGCCAACGTCGATTACGACAAGGCCCTTGGCGTGCGCTGGGGCGGGTCGAGCAACAGAGGC
AATTTCACCACCGGCGGCAGCGGCACCGTGACGGGCGCAGCAGCGACCAACGGTCCCTATGTCGACATGGGCGTGACCGA
TGCCACCTCGTCTATCGGTCTGGGCTTTCTGACCAACAACACGATTCTTGATCTTGAGCTGACGGCCATGGAGAAGAGCG
GCAACGGCGAAGTGGTCTCGCAGCCGAAGGTCGTTACGTCGGACAAGGAAACCGCGAAAATTCTGAAAGGTACGGAAGTG
CCTTATCAGGAAGCGAGCTCCAGCGGCGCGACGTCGGTGTCATTCAAAGAGGCCTCGCTGTCGCTGGAAGTGACCCCGCA
GATCACCCCTGACAACCGCATCATCATGGAGGTCAAGGTCACGAAGGATGAGCCGGACTACGTGAATACCGTGCTGGGCG
TGCCGCCGATCAAGAAAAACGAAGTCAACGCCAAGGTGCTGGTGGCCGATGGCGAGACCATCGTAATTGGTGGCGTGTTC
TCGAATACGCAGAGTAAAGTCGTCGACAAGGTGCCATTTTTGGGCGATGTGCCGTATGTTGGCCGCCTTTTCCGGCGCGA
TGTGGTTTCGGAGTCAAAATCCGAGCTGTTGGTGTTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTC
GTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(529-685)

Bacterial type II/III secretion system short domain (Secretin_N)

(386-451)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(312-358)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.528

100

0.742
  comQ Acinetobacter baylyi ADP1

42.98

100

0.432
  pilQ Acinetobacter baumannii D1279779

38.889

100

0.414
  pilQ Acinetobacter baumannii strain A118

38.889

100

0.414
  pilQ Legionella pneumophila strain Lp02

39.213

98.847

0.388
  pilQ Legionella pneumophila strain ERS1305867

39.213

98.847

0.388