Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FX982_RS09445 Genome accession   NZ_CP053746
Coordinates   2069866..2070930 (+) Length   354 a.a.
NCBI ID   WP_074890782.1    Uniprot ID   A0A1I0G993
Organism   Pseudomonas graminis strain PgKB30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2064866..2075930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX982_RS09435 (FX982_01891) - 2065770..2067038 (+) 1269 WP_122533933.1 malic enzyme-like NAD(P)-binding protein -
  FX982_RS09440 (FX982_01892) - 2067182..2069659 (-) 2478 WP_172610440.1 penicillin-binding protein 1A -
  FX982_RS09445 (FX982_01893) pilM 2069866..2070930 (+) 1065 WP_074890782.1 pilus assembly protein PilM Machinery gene
  FX982_RS09450 (FX982_01894) - 2070930..2071499 (+) 570 WP_122533931.1 PilN domain-containing protein -
  FX982_RS09455 (FX982_01895) pilO 2071496..2072143 (+) 648 WP_172610441.1 type 4a pilus biogenesis protein PilO -
  FX982_RS09460 (FX982_01896) - 2072140..2072667 (+) 528 WP_065986622.1 pilus assembly protein PilP -
  FX982_RS09465 (FX982_01897) pilQ 2072721..2074805 (+) 2085 WP_172610442.1 type IV pilus secretin PilQ Machinery gene
  FX982_RS09470 (FX982_01898) aroK 2074810..2075328 (+) 519 WP_065986620.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38219.81 Da        Isoelectric Point: 4.6363

>NTDB_id=446823 FX982_RS09445 WP_074890782.1 2069866..2070930(+) (pilM) [Pseudomonas graminis strain PgKB30]
MFELFSKKANTLLGIDISSTSVKLLELSRSGNRYKVESYAVEPLPANAVVEKNIAELEGVGSALTRLLVKAKSNVKIAAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLSAQLGQDHEQLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSV
SEAGLAKKQGGLPDDYVMEVLQPFKDAVVQQVSRSLQFFFAAGQYNRVDYIMLAGGTASISGLDRLIQERLGTPTLVANP
FADMALSVKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=446823 FX982_RS09445 WP_074890782.1 2069866..2070930(+) (pilM) [Pseudomonas graminis strain PgKB30]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTGGGGATCGATATTAGCTCCACCTCGGTCAAGCTCCTGGAGCT
AAGTCGTTCCGGCAACCGTTACAAGGTCGAGTCTTATGCGGTCGAGCCACTGCCCGCCAACGCAGTGGTCGAAAAGAACA
TCGCGGAGCTCGAAGGTGTCGGCAGTGCTTTGACGCGTTTGCTGGTAAAAGCCAAGAGTAACGTCAAAATCGCGGCGGTT
GCCGTCGCCGGTTCGGCTGTTATTACCAAGACCATCGAGATGGACGCAGGTCTTTCCGACGACGAGATGGAAAACCAGCT
TAAGATCGAGGCCGATCAGTACATTCCCTATCCGCTCGAAGAAGTCGCCATCGACTTCGAAGTCCAAGGCTATTCGGCGC
GCAATCCCGAACGCGTTGAGGTATTGCTCGCCGCCTGCCGCAAGGAAAACGTAGAAGTGCGCGAAGCCGCGCTGGCACTG
GCCGGGCTCACCGCTCGCGTGGTCGACGTCGAAGCCTATGCCTTGGAGCGCTCGTTCGGTCTGCTGTCGGCGCAATTGGG
CCAGGACCACGAACAGTTGACCGTCGCCGTCATCGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACAACGGCC
GCATCATCTATACGCGTGAACAATTATTTGGCGGTCGCCAGTTAACCGAAGAAATCCAGCGCCGTTATGGCTTGTCGGTC
AGCGAGGCCGGGCTTGCGAAAAAGCAGGGCGGACTCCCCGACGACTACGTTATGGAAGTCCTGCAGCCGTTCAAGGACGC
CGTGGTGCAACAGGTGTCCCGTTCGCTGCAGTTTTTCTTCGCTGCCGGCCAGTACAACCGCGTCGACTACATCATGCTGG
CCGGCGGCACCGCCTCTATTTCCGGTCTTGATCGGCTGATCCAGGAACGCCTGGGCACGCCGACGCTGGTGGCCAACCCC
TTTGCTGACATGGCGCTGAGCGTCAAGGTCAACGCCGGCGCCCTCGCCAGCGATGCTCCCGCACTGATGATCGCTTGCGG
TCTGGCCCTGAGGAGCTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I0G993

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466