Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   I5U70_RS26145 Genome accession   NZ_CP065412
Coordinates   5638746..5639210 (-) Length   154 a.a.
NCBI ID   WP_058147538.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa isolate P33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5633746..5644210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5U70_RS26130 (I5U70_26140) nadC 5636246..5637094 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I5U70_RS26140 (I5U70_26150) - 5637275..5638588 (-) 1314 WP_223697243.1 O-antigen ligase family protein -
  I5U70_RS26145 (I5U70_26155) pilA/pilAI 5638746..5639210 (-) 465 WP_058147538.1 pilin Machinery gene
  I5U70_RS26150 (I5U70_26160) pilB 5639441..5641141 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I5U70_RS26155 (I5U70_26165) pilC 5641145..5642362 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  I5U70_RS26160 (I5U70_26170) pilD 5642363..5643235 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  I5U70_RS26165 (I5U70_26175) coaE 5643232..5643843 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  I5U70_RS26170 (I5U70_26180) yacG 5643840..5644040 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16224.57 Da        Isoelectric Point: 8.9647

>NTDB_id=446822 I5U70_RS26145 WP_058147538.1 5638746..5639210(-) (pilA/pilAI) [Pseudomonas aeruginosa isolate P33]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKGQIKIDKADTATPEISGTLGRYSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=446822 I5U70_RS26145 WP_058147538.1 5638746..5639210(-) (pilA/pilAI) [Pseudomonas aeruginosa isolate P33]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCCGCTATTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.671

100

0.448
  pilA Acinetobacter baumannii strain A118

42.857

100

0.429
  pilA Pseudomonas aeruginosa PAK

41.139

100

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

37.059

100

0.409
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.073

98.052

0.383
  pilA Vibrio cholerae strain A1552

39.073

98.052

0.383
  pilA Vibrio cholerae C6706

39.073

98.052

0.383
  pilA2 Legionella pneumophila strain ERS1305867

37.748

98.052

0.37
  pilA2 Legionella pneumophila str. Paris

37.086

98.052

0.364