Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   FX982_RS09420 Genome accession   NZ_CP053746
Coordinates   2062146..2064365 (-) Length   739 a.a.
NCBI ID   WP_172610438.1    Uniprot ID   A0A6M8MLE3
Organism   Pseudomonas graminis strain PgKB30     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2057146..2069365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX982_RS09400 (FX982_01884) hslU 2057375..2058712 (-) 1338 WP_122533939.1 ATP-dependent protease ATPase subunit HslU -
  FX982_RS09405 (FX982_01885) hslV 2058782..2059312 (-) 531 WP_065986638.1 ATP-dependent protease subunit HslV -
  FX982_RS09410 (FX982_01886) - 2059589..2060278 (-) 690 WP_172610436.1 SPOR domain-containing protein -
  FX982_RS09415 (FX982_01887) argS 2060280..2062025 (-) 1746 WP_172610437.1 arginine--tRNA ligase -
  FX982_RS09420 (FX982_01888) priA 2062146..2064365 (-) 2220 WP_172610438.1 primosomal protein N' Machinery gene
  FX982_RS09425 (FX982_01889) rpmE 2064534..2064764 (+) 231 WP_122533935.1 50S ribosomal protein L31 -
  FX982_RS09430 (FX982_01890) - 2064788..2065591 (+) 804 WP_172610439.1 thermonuclease family protein -
  FX982_RS09435 (FX982_01891) - 2065770..2067038 (+) 1269 WP_122533933.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81572.92 Da        Isoelectric Point: 8.1796

>NTDB_id=446822 FX982_RS09420 WP_172610438.1 2062146..2064365(-) (priA) [Pseudomonas graminis strain PgKB30]
MPDAILRLALPSPLRRLFDYLAPAGVARSALQPGMRLRVPFGRREMIGVLVEVVDHSDVPADKLKPAIAVLDAEPPLPPA
LFKLCLWTAQYYQHSLGDTLSWALPVLLRQGEPAEARQERFWHAAPDASVDDPRIARAPKQKEALRTLAQHPHGVAHQLL
SKLMLNRDSLNLLLAKGLVTVEVRGHGPVERHEHWLAQPELPLNIEQRAAYEAIRSGFGSFHASLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSAVNDRDRLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRAHQEDIPIVLGSATPSLESLHNAHTGRYALLRLNQRAGGAQQPRFLRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWLSECPRCDARMTVHQRSGELRCHHCGHVERVPRNC
PACGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFATIQKGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRAAGLPPFSHL
ALLRAEAHKPGQAEGFLDEACTEAERLLKDLALGGIELLGPVPAPMERRAGRYRAQLLVQANARAPLHKLLATWLLTLEN
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=446822 FX982_RS09420 WP_172610438.1 2062146..2064365(-) (priA) [Pseudomonas graminis strain PgKB30]
GTGCCCGACGCCATCTTGCGCCTCGCCCTGCCCTCGCCGCTGCGTCGCCTGTTCGACTACCTTGCCCCCGCAGGTGTGGC
GCGCAGTGCGTTGCAACCGGGGATGCGCCTGCGCGTGCCGTTCGGTCGTCGGGAAATGATTGGCGTGCTGGTGGAAGTCG
TCGATCACAGTGACGTGCCGGCCGACAAGCTCAAGCCGGCCATTGCCGTGCTGGATGCCGAACCGCCGCTGCCGCCCGCA
CTGTTCAAGCTGTGCCTGTGGACGGCCCAGTATTACCAGCACAGCCTGGGCGACACCCTGAGTTGGGCGTTGCCGGTGTT
GCTGCGTCAGGGCGAACCGGCGGAAGCGCGGCAGGAGCGGTTCTGGCACGCAGCCCCGGACGCGAGCGTCGATGACCCGC
GCATCGCCCGGGCGCCTAAACAAAAGGAAGCGCTGCGCACGCTGGCCCAGCACCCCCATGGCGTGGCTCACCAGTTGCTG
AGCAAGCTGATGCTCAACCGGGACAGCCTGAATCTGTTGCTCGCCAAAGGACTGGTGACCGTCGAGGTGCGCGGTCATGG
GCCGGTTGAGCGACACGAGCACTGGCTGGCGCAGCCGGAATTGCCGCTCAACATCGAGCAACGGGCTGCCTATGAAGCCA
TTCGGTCGGGTTTCGGCAGTTTCCACGCTTCACTGCTGGCGGGGGTCACCGGCAGCGGCAAGACCGAAGTGTATCTGCAG
CTCATCCGCGAAACGCTGGAAGCGGGCAAGCAGGCGCTGGTGTTGATTCCGGAAATCAATTTGGGGCCGCAGACCCTCGC
GCGCTTCGAGCAGCGTTTCAACGCGCGCATTGCGCTGCTGCATTCGGCGGTCAACGACCGCGACCGGCTGGACGCCTGGC
TGGCAGCACGCGATGGCGAAGCCGACATCATCATCGGTACCCGTTCGGCGCTGTTCACCCCGATGAAAAACCCGGGGCTG
ATCATCATCGACGAGGAGCACGACGGCTCCTATAAACAGCAGGAGGGTCTGCGCTATCACGCCCGGGACCTGGCGCTGGT
GAGGGCGCATCAGGAGGACATCCCGATCGTGCTCGGCTCAGCGACGCCTTCGCTGGAAAGCCTGCACAACGCCCACACCG
GTCGGTATGCGCTCCTGCGTCTGAATCAGCGCGCGGGTGGCGCTCAGCAACCGCGCTTCCTGCGGCTGGATGTGAAAAGT
CGCCCCCTCGACAGCGGCATTTCAGGCCCCATGCAGCAAGCCATCGGCCAGACCCTGGCGGCGGGCCAGCAAGTGCTGGT
GTTCCTCAATCGACGCGGCTTTGCCCCTACGCTGCTGTGTCACGACTGTGGCTGGTTGTCGGAATGCCCCCGCTGCGACG
CGCGCATGACCGTTCACCAGCGCTCGGGCGAGCTGCGCTGTCACCACTGCGGCCATGTCGAGCGGGTGCCGCGCAATTGC
CCGGCGTGTGGCAAGGTCGATCTGCGCCCGGTGGGTGCAGGGACCGAACGAGCCGAGGAGCGGCTGGCGATTCTGTTTCC
CGACTACCCGGTGCTGCGCGTCGATCGCGACAGTACCTCGCGCAAGGACGCGATGAATCAGCTGTTCGCAACCATACAGA
AAGGGCAGCCGTGCATTCTGGTGGGCACACAGATGCTCGCCAAAGGGCATCACTTCCCGCGCGTGACGCTGGTGTCGATT
CTCGATGCCGATGGCGGGTTGTTTTCCGGTGACTTCCGCGCCAGCGAGCGCATGGCGCAATTGATTGTGCAGGTCGCAGG
CAGAGCCGGGCGCGCCGAAGAGCCGGGCAAGGTGATCATCCAAACCCACCTGGCCGACCATCCTTTATTGATTCAACTGA
CCGAACAGGGTTATTTCGCCTTTGCCGAGCAGGCGCTGAGTGAGCGTCGCGCCGCCGGGCTGCCGCCGTTCTCGCACTTG
GCACTGCTGCGCGCCGAAGCGCACAAGCCGGGTCAGGCCGAGGGATTTCTCGACGAAGCCTGCACCGAAGCCGAGCGCCT
GTTGAAGGACCTGGCCCTGGGCGGCATCGAGCTGTTGGGCCCGGTTCCGGCGCCGATGGAGCGACGCGCCGGACGCTATC
GCGCTCAGTTGCTGGTCCAGGCCAACGCCCGCGCGCCGCTGCACAAGCTGCTCGCGACCTGGCTGCTGACACTGGAAAAC
ATGCCCAGCGGGCGCCAGGTCAGATGGTCGCTGGACGTGGATCCGGTGGACCTTTATTGA

Domains

Predicted by InterproScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M8MLE3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.651

100

0.48