Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   IUD20_RS10335 Genome accession   NZ_CP064334
Coordinates   2292457..2292897 (+) Length   146 a.a.
NCBI ID   WP_004090375.1    Uniprot ID   B2I9R0
Organism   Xylella fastidiosa subsp. fastidiosa strain Bakersfield-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2287457..2297897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUD20_RS10310 (IUD20_10310) coaE 2287554..2288177 (-) 624 WP_004090385.1 dephospho-CoA kinase -
  IUD20_RS10315 (IUD20_10315) pilD 2288189..2289052 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  IUD20_RS10320 (IUD20_10320) pilC 2289059..2290261 (-) 1203 WP_011098309.1 type II secretion system F family protein Machinery gene
  IUD20_RS10325 (IUD20_10325) pilA 2290702..2291148 (+) 447 WP_004090380.1 pilin Machinery gene
  IUD20_RS10330 (IUD20_10330) - 2291394..2291603 (-) 210 WP_004090377.1 hypothetical protein -
  IUD20_RS10335 (IUD20_10335) pilA/pilAI 2292457..2292897 (+) 441 WP_004090375.1 pilin Machinery gene
  IUD20_RS10340 (IUD20_10340) pilB 2293740..2295473 (+) 1734 WP_004090374.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IUD20_RS10345 (IUD20_10345) - 2295567..2296979 (-) 1413 WP_004090373.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14977.25 Da        Isoelectric Point: 8.1165

>NTDB_id=442873 IUD20_RS10335 WP_004090375.1 2292457..2292897(+) (pilA/pilAI) [Xylella fastidiosa subsp. fastidiosa strain Bakersfield-8]
MKKQQGFTLIELMIVIAIIAILAAIALPMYQNYVARSQVAAALAEITPGRTQAEIRMADGQASNTPNAIGLRSPTTRCSL
VVVDITTTGGAIVCTMIGNGQVNNQTITLTRIADNNAGQGGVNTGGNWTCTTTAPAALTPAGCTGT

Nucleotide


Download         Length: 441 bp        

>NTDB_id=442873 IUD20_RS10335 WP_004090375.1 2292457..2292897(+) (pilA/pilAI) [Xylella fastidiosa subsp. fastidiosa strain Bakersfield-8]
ATGAAAAAGCAACAAGGCTTCACTTTGATCGAGCTGATGATCGTCATTGCGATCATCGCCATCCTGGCAGCGATCGCTCT
GCCCATGTACCAAAATTATGTCGCCAGGTCTCAAGTCGCAGCGGCACTAGCCGAGATCACACCGGGCCGAACACAAGCCG
AAATCCGTATGGCTGATGGACAGGCTTCAAACACTCCTAACGCTATTGGACTACGCAGCCCGACCACTCGTTGCAGCCTC
GTTGTGGTGGACATCACAACTACTGGCGGCGCAATCGTATGTACGATGATTGGTAATGGTCAGGTCAACAATCAAACTAT
CACCTTGACCCGCATCGCTGATAACAACGCAGGCCAAGGTGGCGTCAATACTGGGGGGAACTGGACCTGCACCACAACCG
CGCCTGCAGCGTTAACTCCAGCAGGCTGCACTGGAACTTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B2I9R0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.27

100

0.459
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.466

100

0.425
  pilA Acinetobacter baumannii strain A118

41.216

100

0.418
  pilA Pseudomonas aeruginosa PAK

37.736

100

0.411
  pilA Vibrio cholerae C6706

37.908

100

0.397
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.908

100

0.397
  pilA Vibrio cholerae strain A1552

37.908

100

0.397
  pilA Haemophilus influenzae 86-028NP

37.162

100

0.377
  comP Acinetobacter baylyi ADP1

35.256

100

0.377
  pilA Vibrio parahaemolyticus RIMD 2210633

37.241

99.315

0.37