Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HKW97_RS04370 Genome accession   NZ_CP053063
Coordinates   928117..929334 (-) Length   405 a.a.
NCBI ID   WP_197872018.1    Uniprot ID   -
Organism   Pseudomonas luteola strain JZ42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 923117..934334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKW97_RS04340 (HKW97_04320) - 923785..925083 (-) 1299 WP_197872023.1 NAD(P)/FAD-dependent oxidoreductase -
  HKW97_RS04345 (HKW97_04325) - 925188..925670 (-) 483 WP_125887166.1 MOSC domain-containing protein -
  HKW97_RS04350 (HKW97_04330) - 925874..926347 (+) 474 WP_010797203.1 hypothetical protein -
  HKW97_RS04355 (HKW97_04335) yacG 926438..926641 (-) 204 WP_010797204.1 DNA gyrase inhibitor YacG -
  HKW97_RS04360 (HKW97_04340) coaE 926634..927248 (-) 615 WP_407362913.1 dephospho-CoA kinase -
  HKW97_RS04365 (HKW97_04345) pilD 927248..928117 (-) 870 WP_407362914.1 prepilin peptidase Machinery gene
  HKW97_RS04370 (HKW97_04350) pilC 928117..929334 (-) 1218 WP_197872018.1 type II secretion system F family protein Machinery gene
  HKW97_RS04375 (HKW97_04355) pilB 929466..931151 (-) 1686 WP_197872017.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HKW97_RS04380 (HKW97_04360) pilA/pilAI 931371..931763 (+) 393 WP_407362915.1 pilin Machinery gene
  HKW97_RS04385 (HKW97_04365) - 931817..933544 (+) 1728 WP_407362916.1 O-antigen ligase family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44311.09 Da        Isoelectric Point: 9.6385

>NTDB_id=442629 HKW97_RS04370 WP_197872018.1 928117..929334(-) (pilC) [Pseudomonas luteola strain JZ42]
MADKALKMSAFTWEGTDRKGVKIKGEMTGLNPALVKAQLRKQGINPTRVRKKSVSIFSRGKKIKPMDIALFTRQLATMLG
AGVPLLQSFDIISEGFDNPNMRKLVDDVKQDVAAGNSLSSSLRKKPQYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
EQLKKKIKKAMTYPAAVVVVAIVVSAILLIKVVPQFQSVFEGFGAELPAFTLMVIGLSETLQEWWFIVLLALFALGFGFQ
MAHKRSEKFRDAVDRFVLKIPIVGAILYKSAIARYARTLSTTFAAGVPLVDALDSVSGATGNVVFRNAVDKIKQDVSTGM
QLNFSMRTTAVFTSMAVQMTAIGEESGALDEMLDKVATFYEEEVDNMVDSLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=442629 HKW97_RS04370 WP_197872018.1 928117..929334(-) (pilC) [Pseudomonas luteola strain JZ42]
ATGGCCGACAAAGCATTAAAAATGAGCGCTTTTACCTGGGAGGGAACGGACCGTAAAGGCGTCAAGATCAAGGGCGAAAT
GACCGGGCTGAACCCTGCCCTGGTCAAGGCCCAACTGCGCAAGCAAGGGATCAACCCCACGCGCGTACGCAAGAAATCAG
TCTCGATCTTCAGCCGCGGCAAAAAGATCAAGCCGATGGACATCGCCCTGTTCACTCGCCAGCTGGCTACCATGCTCGGG
GCAGGTGTTCCTTTGCTGCAATCGTTCGACATTATCAGCGAAGGCTTTGATAACCCGAACATGCGCAAACTGGTCGATGA
CGTCAAGCAGGACGTGGCAGCGGGTAACAGCCTGTCTAGCTCCCTGCGTAAAAAGCCCCAATACTTTGATGACCTCTACT
GCAACCTGGTCGATGCGGGTGAGCAATCTGGTGCGCTGGAAACGCTGCTGGATCGCGTAGCGACCTACAAAGAAAAAACC
GAGCAGCTCAAGAAAAAAATCAAAAAGGCTATGACCTACCCTGCCGCGGTCGTTGTGGTAGCGATTGTGGTTTCAGCCAT
TCTGTTGATCAAGGTTGTACCTCAGTTCCAATCCGTATTCGAGGGCTTTGGTGCAGAGCTACCTGCGTTTACGTTAATGG
TGATTGGCCTTTCAGAAACCCTGCAGGAATGGTGGTTCATCGTTCTGCTGGCTCTTTTCGCGTTGGGTTTCGGCTTTCAG
ATGGCACACAAAAGGTCTGAAAAATTCCGCGATGCCGTTGACCGCTTTGTACTCAAAATACCAATTGTAGGCGCCATTCT
TTATAAGTCTGCGATTGCACGCTATGCACGTACCTTATCTACCACCTTTGCTGCGGGTGTACCGCTGGTGGACGCGTTGG
ACTCGGTTTCCGGCGCTACGGGTAACGTGGTCTTCCGTAATGCCGTCGACAAGATCAAGCAAGATGTCTCAACCGGTATG
CAGCTGAACTTCTCCATGCGAACCACCGCGGTATTTACTTCAATGGCGGTGCAAATGACGGCCATTGGTGAGGAGTCGGG
TGCACTGGACGAAATGCTGGATAAAGTGGCCACGTTTTATGAAGAAGAAGTGGACAACATGGTCGATAGCCTTACCGCTC
TGATGGAGCCCATGATCATGGCGGTTCTGGGCGTACTGGTGGGCGGTTTGATCATCGCCATGTATCTTCCTATCTTCCAG
TTGGGCTCGGTTGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

80.988

100

0.81
  pilC Acinetobacter baumannii D1279779

61.634

99.753

0.615
  pilC Acinetobacter baylyi ADP1

61.084

100

0.612
  pilC Legionella pneumophila strain ERS1305867

54.798

97.778

0.536
  pilG Neisseria meningitidis 44/76-A

45.074

100

0.452
  pilG Neisseria gonorrhoeae MS11

45.074

100

0.452
  pilC Vibrio cholerae strain A1552

42.716

100

0.427
  pilC Vibrio campbellii strain DS40M4

41.872

100

0.42
  pilC Thermus thermophilus HB27

38.71

99.506

0.385