Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HKW97_RS04375 Genome accession   NZ_CP053063
Coordinates   929466..931151 (-) Length   561 a.a.
NCBI ID   WP_197872017.1    Uniprot ID   -
Organism   Pseudomonas luteola strain JZ42     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 924466..936151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKW97_RS04345 (HKW97_04325) - 925188..925670 (-) 483 WP_125887166.1 MOSC domain-containing protein -
  HKW97_RS04350 (HKW97_04330) - 925874..926347 (+) 474 WP_010797203.1 hypothetical protein -
  HKW97_RS04355 (HKW97_04335) yacG 926438..926641 (-) 204 WP_010797204.1 DNA gyrase inhibitor YacG -
  HKW97_RS04360 (HKW97_04340) coaE 926634..927248 (-) 615 WP_407362913.1 dephospho-CoA kinase -
  HKW97_RS04365 (HKW97_04345) pilD 927248..928117 (-) 870 WP_407362914.1 prepilin peptidase Machinery gene
  HKW97_RS04370 (HKW97_04350) pilC 928117..929334 (-) 1218 WP_197872018.1 type II secretion system F family protein Machinery gene
  HKW97_RS04375 (HKW97_04355) pilB 929466..931151 (-) 1686 WP_197872017.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HKW97_RS04380 (HKW97_04360) pilA/pilAI 931371..931763 (+) 393 WP_407362915.1 pilin Machinery gene
  HKW97_RS04385 (HKW97_04365) - 931817..933544 (+) 1728 WP_407362916.1 O-antigen ligase family protein -
  HKW97_RS04395 (HKW97_04375) - 934157..934555 (-) 399 WP_407362917.1 hypothetical protein -
  HKW97_RS04400 (HKW97_04380) - 934554..935407 (+) 854 Protein_862 IS5 family transposase -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62184.39 Da        Isoelectric Point: 5.5952

>NTDB_id=442630 HKW97_RS04375 WP_197872017.1 929466..931151(-) (pilB) [Pseudomonas luteola strain JZ42]
MNDLAPLSGLARQLVLAELLDEKTAQSAVAQSKVSKVPLVTHLVQNRLVKSRAIAELSAEQFGLAFLDLSAVDKESLPKE
IVSEKLMQQHHFLPLAKRGNKLFIGISDPTNQQAINDIQFSTGLTAEPILVEDDKLHNAIDQYLDNGTGGLDLGDIDLDI
EINTEEEKKSEVDADDAPVVRFVNKMLLDAIKRGSSDLHFEPYEKMYRVRFRTDGVMHEMARPPIQLAPRISARLKVMAS
MDISERRKPQDGRVKLKVSKNKSIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEERQKELYMEALSQPQGMILVT
GPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRDLETAEI
AIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPKCKKPHDVPRETLLSEGFPESQIGK
FTLYSPIGCEECNNGYRGRVGIYEVVKITSAMQNLIMEEGNSIEIARKAREEGFNDLRTSGLLKAMQGVTSLEEVNRVTK
D

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=442630 HKW97_RS04375 WP_197872017.1 929466..931151(-) (pilB) [Pseudomonas luteola strain JZ42]
ATGAATGATTTAGCGCCCCTCTCTGGTCTTGCCCGTCAGCTTGTTTTGGCGGAGCTACTGGATGAAAAAACGGCTCAAAG
CGCGGTTGCGCAGAGCAAGGTCAGTAAAGTCCCATTGGTGACGCACCTGGTCCAGAATCGCCTCGTTAAAAGCCGGGCGA
TTGCGGAGCTGTCTGCTGAACAATTCGGCTTGGCGTTTTTAGACCTCAGCGCAGTTGATAAGGAGTCCTTGCCCAAGGAG
ATCGTGAGCGAGAAATTGATGCAGCAGCATCACTTTCTGCCGCTCGCCAAACGTGGCAATAAGCTTTTTATTGGCATATC
CGACCCAACCAATCAGCAAGCGATCAATGACATTCAGTTCAGCACAGGGCTGACTGCCGAACCCATACTGGTTGAGGATG
ACAAGCTTCATAATGCCATTGATCAGTACTTGGATAACGGCACCGGTGGGCTGGATCTCGGCGATATTGACCTCGATATC
GAGATCAATACAGAAGAAGAGAAGAAGTCTGAAGTAGACGCGGACGATGCCCCAGTTGTCCGCTTCGTCAACAAAATGCT
GCTCGACGCCATCAAACGCGGCTCGTCTGACCTACACTTTGAGCCTTATGAGAAGATGTACCGAGTGCGCTTCCGTACCG
ATGGTGTCATGCATGAAATGGCACGCCCGCCTATTCAGCTGGCCCCACGCATTTCAGCCCGCCTGAAGGTGATGGCGAGT
ATGGATATCTCCGAGCGCCGCAAGCCACAGGACGGCCGGGTAAAGCTCAAGGTTTCCAAAAACAAGTCCATCGATTTTCG
TGTCAACACGCTTCCAACCCTCTGGGGCGAGAAGATCGTAATGCGAATTCTCGATCCCTCCAGTGCCCAGATGGGTATTG
ATGCCTTGGGCTATGAAGAGCGCCAGAAAGAGCTGTATATGGAGGCGCTTAGCCAACCACAAGGTATGATTCTGGTAACC
GGCCCCACGGGCTCCGGTAAAACCGTATCGCTGTACACAGGCTTGAACATCCTTAATACGGTCGACATCAATATCTCGAC
GGCAGAAGACCCGGTAGAGATCAACCTGGAGGGTATCAACCAGGTTAATGTCAACCCGCGTCAAGGAATGGACTTTGCCC
AAGCGCTTAGAGCGTTTCTCCGTCAAGATCCTGACGTGATCATGGTGGGTGAAATCCGCGACCTTGAAACGGCAGAGATT
GCCATCAAGGCTGCACAAACGGGCCACATGGTTATGTCGACGCTGCATACGAACAGCGCTGCAGAAACACTGACGCGCCT
GCGGAACATGGGCGTACCTGCCTTTAACATTGCCACCTCGGTAAACCTGATCATTGCCCAGCGGCTGGCTCGCAAACTGT
GCCCTAAATGCAAAAAGCCGCATGATGTGCCTAGGGAAACCCTGTTGAGTGAAGGTTTCCCTGAAAGCCAGATCGGCAAG
TTTACGCTGTACTCACCTATTGGTTGCGAGGAATGCAATAACGGCTACCGTGGCCGTGTAGGCATTTATGAAGTAGTAAA
GATTACATCCGCCATGCAAAACCTGATTATGGAAGAAGGCAACTCGATCGAGATTGCCCGCAAGGCGCGTGAAGAAGGCT
TTAATGACCTACGGACATCTGGCCTGCTCAAGGCCATGCAAGGTGTAACCAGCCTGGAAGAAGTAAACCGCGTCACCAAG
GACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(185-452)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.363

100

0.585
  pilB Acinetobacter baylyi ADP1

58.289

100

0.583
  pilB Legionella pneumophila strain ERS1305867

53.263

100

0.538
  pilB Vibrio cholerae strain A1552

50.089

100

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

50

99.109

0.496
  pilB Vibrio campbellii strain DS40M4

48.845

100

0.49
  pilF Neisseria gonorrhoeae MS11

48.833

99.287

0.485
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.662

95.9

0.371
  pilF Thermus thermophilus HB27

37.143

99.822

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

40.232

92.157

0.371