Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HKW97_RS04365 Genome accession   NZ_CP053063
Coordinates   927248..928117 (-) Length   289 a.a.
NCBI ID   WP_407362914.1    Uniprot ID   -
Organism   Pseudomonas luteola strain JZ42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 922248..933117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKW97_RS04340 (HKW97_04320) - 923785..925083 (-) 1299 WP_197872023.1 NAD(P)/FAD-dependent oxidoreductase -
  HKW97_RS04345 (HKW97_04325) - 925188..925670 (-) 483 WP_125887166.1 MOSC domain-containing protein -
  HKW97_RS04350 (HKW97_04330) - 925874..926347 (+) 474 WP_010797203.1 hypothetical protein -
  HKW97_RS04355 (HKW97_04335) yacG 926438..926641 (-) 204 WP_010797204.1 DNA gyrase inhibitor YacG -
  HKW97_RS04360 (HKW97_04340) coaE 926634..927248 (-) 615 WP_407362913.1 dephospho-CoA kinase -
  HKW97_RS04365 (HKW97_04345) pilD 927248..928117 (-) 870 WP_407362914.1 prepilin peptidase Machinery gene
  HKW97_RS04370 (HKW97_04350) pilC 928117..929334 (-) 1218 WP_197872018.1 type II secretion system F family protein Machinery gene
  HKW97_RS04375 (HKW97_04355) pilB 929466..931151 (-) 1686 WP_197872017.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HKW97_RS04380 (HKW97_04360) pilA/pilAI 931371..931763 (+) 393 WP_407362915.1 pilin Machinery gene

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 31897.82 Da        Isoelectric Point: 6.6804

>NTDB_id=442628 HKW97_RS04365 WP_407362914.1 927248..928117(-) (pilD) [Pseudomonas luteola strain JZ42]
MEAIELLATYPAAYLGCALALGLLVGSFLNVVVHRLPKMLERDWIAQSRQMLELPSEGEAEPYNLAKPHSHCPHCQHRIR
PWENIPVISYVMLRGKCSSCKAPISVRYPLVELTCGLLSLFVVWHFGFTWQAASALLLTWGLLAASLIDADHQILPDVIV
LPLLWVGLLVNQFELFSSLSDAVWGAAAGYMSLWVVFQLFRLITGKEGMGFGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGIILLRLKNAENSTPIPFGPYLAIAGWIALLWGDQITGMYLHFAGF

Nucleotide


Download         Length: 870 bp        

>NTDB_id=442628 HKW97_RS04365 WP_407362914.1 927248..928117(-) (pilD) [Pseudomonas luteola strain JZ42]
ATGGAGGCTATCGAGCTTTTGGCAACCTACCCCGCCGCGTACTTAGGTTGTGCGTTGGCACTGGGCTTATTGGTTGGCAG
CTTTCTCAATGTAGTCGTACACCGGCTTCCCAAAATGCTTGAGCGGGACTGGATAGCCCAGTCTCGCCAAATGCTGGAGC
TACCCAGCGAAGGCGAAGCAGAGCCCTATAACCTTGCCAAGCCCCACTCCCATTGCCCTCACTGCCAACATCGCATTCGA
CCGTGGGAAAACATTCCTGTTATCAGCTATGTCATGCTGCGCGGCAAGTGCTCCAGCTGTAAGGCCCCTATCAGCGTTCG
CTATCCGCTGGTTGAATTGACGTGCGGCCTGCTTTCCCTGTTTGTTGTCTGGCATTTTGGCTTTACCTGGCAGGCAGCCA
GCGCCTTGTTACTGACTTGGGGACTGCTCGCCGCCAGCCTGATCGATGCCGATCACCAGATTCTTCCAGACGTCATTGTT
CTTCCGCTGCTATGGGTTGGCTTGCTGGTTAACCAGTTTGAGTTGTTCTCTAGTCTCTCCGATGCAGTCTGGGGTGCAGC
GGCGGGCTATATGAGCTTGTGGGTGGTTTTCCAGCTTTTCAGGCTGATCACAGGCAAGGAAGGCATGGGCTTTGGTGACT
TCAAGCTGCTGGCCATGCTGGGCGCCTGGGGCGGCTGGCAAATCCTGCCGCTTACGATATTGCTGTCCTCTTTAGTGGGC
GCGATTCTCGGCATCATTCTGCTTCGCCTCAAGAATGCCGAAAACAGCACCCCGATACCGTTTGGGCCTTATCTGGCCAT
TGCGGGGTGGATTGCTTTGCTTTGGGGTGATCAAATAACAGGCATGTACCTTCACTTTGCCGGTTTTTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

54.909

95.156

0.522
  pilD Vibrio cholerae strain A1552

52.797

98.962

0.522
  pilD Acinetobacter nosocomialis M2

53.818

95.156

0.512
  pilD Vibrio campbellii strain DS40M4

49.117

97.924

0.481
  pilD Neisseria gonorrhoeae MS11

47.909

91.003

0.436