Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   DB356_RS04355 Genome accession   NZ_CP053030
Coordinates   962479..962901 (+) Length   140 a.a.
NCBI ID   WP_214423659.1    Uniprot ID   -
Organism   Pseudomonas congelans strain 508     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 957479..967901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DB356_RS04330 (DB356_04625) yacG 957639..957848 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  DB356_RS04335 (DB356_04630) coaE 957845..958468 (-) 624 WP_010437862.1 dephospho-CoA kinase -
  DB356_RS04340 (DB356_04635) pilD 958465..959337 (-) 873 WP_122694365.1 A24 family peptidase Machinery gene
  DB356_RS04345 (DB356_04640) pilC 959339..960556 (-) 1218 WP_032613636.1 type II secretion system F family protein Machinery gene
  DB356_RS04350 (DB356_04645) pilB 960559..962253 (-) 1695 WP_214424193.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DB356_RS04355 (DB356_04650) pilA2 962479..962901 (+) 423 WP_214423659.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DB356_RS04360 (DB356_04655) - 963140..963499 (-) 360 WP_214423660.1 helix-turn-helix domain-containing protein -
  DB356_RS04365 (DB356_04660) gstA 963656..964276 (+) 621 WP_214424194.1 glutathione transferase GstA -
  DB356_RS04370 (DB356_04665) - 964519..965211 (+) 693 WP_348772816.1 DUF433 domain-containing protein -
  DB356_RS04375 (DB356_04670) - 965208..965615 (+) 408 WP_214423661.1 hypothetical protein -
  DB356_RS04380 (DB356_04675) - 965659..966204 (-) 546 WP_032702604.1 DUF1523 family protein -
  DB356_RS04385 (DB356_04680) - 966324..967322 (+) 999 WP_214423662.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14006.27 Da        Isoelectric Point: 6.8896

>NTDB_id=442292 DB356_RS04355 WP_214423659.1 962479..962901(+) (pilA2) [Pseudomonas congelans strain 508]
MNVQKGFTLIELMIVVAIVGILAAMAIPQYQDYTMRARFSDVVSVANTYQTAVAVCIQTQGVADNCTTGNNGVPASQVTP
NVASVAVAAGGIITVTPTATTSAASTLVLTPVVGAGAITWSKAGGGCLAAHAPAPILCTL

Nucleotide


Download         Length: 423 bp        

>NTDB_id=442292 DB356_RS04355 WP_214423659.1 962479..962901(+) (pilA2) [Pseudomonas congelans strain 508]
ATGAATGTACAGAAAGGTTTTACGCTGATCGAATTGATGATCGTAGTCGCGATCGTTGGCATTTTGGCGGCGATGGCTAT
TCCGCAATATCAGGATTACACGATGAGAGCACGCTTCTCTGATGTTGTTTCGGTAGCCAATACCTACCAAACTGCAGTTG
CTGTTTGCATACAGACCCAAGGTGTGGCTGACAACTGCACCACTGGAAACAATGGTGTTCCTGCGTCCCAAGTCACACCT
AACGTGGCAAGCGTCGCAGTAGCGGCCGGCGGCATAATTACAGTTACTCCTACGGCTACAACAAGCGCGGCGTCTACGTT
GGTTCTAACCCCTGTAGTCGGTGCAGGTGCAATTACGTGGTCAAAAGCTGGTGGTGGCTGCTTGGCTGCACATGCTCCAG
CGCCTATTCTTTGCACGCTTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(36-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

48.227

100

0.486
  pilA2 Legionella pneumophila strain ERS1305867

46.809

100

0.471
  comP Acinetobacter baylyi ADP1

45.802

93.571

0.429
  pilA Ralstonia pseudosolanacearum GMI1000

45.312

91.429

0.414
  comF Acinetobacter baylyi ADP1

38.519

96.429

0.371
  pilA Haemophilus influenzae Rd KW20

38.806

95.714

0.371
  pilE Neisseria gonorrhoeae strain FA1090

36.111

100

0.371
  pilA Haemophilus influenzae 86-028NP

37.5

97.143

0.364
  pilA Glaesserella parasuis strain SC1401

37.5

97.143

0.364