Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DB356_RS04350 Genome accession   NZ_CP053030
Coordinates   960559..962253 (-) Length   564 a.a.
NCBI ID   WP_214424193.1    Uniprot ID   -
Organism   Pseudomonas congelans strain 508     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 955559..967253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DB356_RS04315 (DB356_04610) - 955765..956298 (-) 534 WP_003317783.1 MOSC domain-containing protein -
  DB356_RS04320 (DB356_04615) - 956295..956924 (-) 630 WP_122694368.1 DUF1780 domain-containing protein -
  DB356_RS04325 (DB356_04620) - 957051..957512 (+) 462 WP_010437859.1 hypothetical protein -
  DB356_RS04330 (DB356_04625) yacG 957639..957848 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  DB356_RS04335 (DB356_04630) coaE 957845..958468 (-) 624 WP_010437862.1 dephospho-CoA kinase -
  DB356_RS04340 (DB356_04635) pilD 958465..959337 (-) 873 WP_122694365.1 A24 family peptidase Machinery gene
  DB356_RS04345 (DB356_04640) pilC 959339..960556 (-) 1218 WP_032613636.1 type II secretion system F family protein Machinery gene
  DB356_RS04350 (DB356_04645) pilB 960559..962253 (-) 1695 WP_214424193.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DB356_RS04355 (DB356_04650) pilA2 962479..962901 (+) 423 WP_214423659.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DB356_RS04360 (DB356_04655) - 963140..963499 (-) 360 WP_214423660.1 helix-turn-helix domain-containing protein -
  DB356_RS04365 (DB356_04660) gstA 963656..964276 (+) 621 WP_214424194.1 glutathione transferase GstA -
  DB356_RS04370 (DB356_04665) - 964519..965211 (+) 693 WP_348772816.1 DUF433 domain-containing protein -
  DB356_RS04375 (DB356_04670) - 965208..965615 (+) 408 WP_214423661.1 hypothetical protein -
  DB356_RS04380 (DB356_04675) - 965659..966204 (-) 546 WP_032702604.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62601.85 Da        Isoelectric Point: 6.2552

>NTDB_id=442291 DB356_RS04350 WP_214424193.1 960559..962253(-) (pilB) [Pseudomonas congelans strain 508]
MTDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASEQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIAISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSDSGLGNLEDVDLGLD
IEPSSDKETSLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKSIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYMEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPESK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=442291 DB356_RS04350 WP_214424193.1 960559..962253(-) (pilB) [Pseudomonas congelans strain 508]
ATGACTGATGCTGTCCTCACTGGCCTGGCCAAGCAATTGGTCCTTGCCGAGCTCCTTACTGAACCTGTTGCGCAGCAAGC
GTATCAACAGGCGCGTCGTGACAAGATTTCATTGGTCAGCTATCTGGTTCAGAACAAACTGGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCCGAGCAGTTTGGCGTGCCTTTTATGGACCTCGCCAGTCTGGACAAGGAAAGCCAGCCCAAGGGCTTG
GTGAGCGAAAAACTGGTTCGACAGCATCACGCCCTCCCACTCTGGCGACGCGGCAACAAGCTGTTCATCGCCATATCAGA
CCCGACCAACCATCAGGCAGTGACTGATATTCAGTTCAGCACCGGCCTGAATACCGAAGCAATTTTGGTCGAAGATGACA
AGCTGACTACCGCAATAGATCGGTTCTTTGACAGTGATAGCGGTCTGGGCAATCTGGAAGATGTTGATCTGGGCCTTGAT
ATCGAGCCTTCTAGTGACAAGGAAACCTCGCTTGCCACACAAAATGACGCAGATGATGCTCCGGTGGTGCGATTCGTCAA
TAAGATGCTAATGGATGCTATCCGTTTGGGATCATCCGATCTGCACTTCGAGCCCTACGAAAAGATCTTCCGCGTGCGCT
TGCGTACCGATGGCATTCTGCATGAAGTAGCCAGGCCGCCAATTCATCTGGCAAATCGTATTGCCGCCCGCCTGAAAGTC
ATGGCCAGCCTCGATATCTCGGAACGGCGCAAACCGCAAGACGGTCGGGTCAAGTTGCGGGTATCCAAAACAAAATCAAT
CGACTTTCGTATGAACACCTTGCCTACGCTATGGGGCGAGAAGATTGTGATGCGGATACTCGATCCGACCAGCGCGCAGA
TGGGCATCGATGCTCTGGGCTACGAGCCAGAACAAAAAGCACTGTATATGGAGGCGCTGAAACAGCCGCAAGGCATGATC
CTCGTGACCGGCCCGACGGGGTCGGGCAAGACTGTTTCCCTGTACACCGGCCTGAATATCCTCAACACCGTGGACATCAA
CATATCCACGGCTGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGCGTCAGGGACTGG
ACTTTTCACAGGCACTGCGCGCATTCCTGCGTCAGGATCCCGACGTAATCATGGTCGGTGAGATACGAGACTTGGAAACC
GCCGAAATTGCCATCAAGGCATCGCAAACCGGACACATGGTGCTCTCGACGCTGCACACCAACAGCGCGGCAGAAACCTT
AACCCGCCTGCACCATATGGGTGTCGCAGCATTCAACATTGCCACAGCGATAAATCTGATCATCGCTCAACGACTTGCAC
GCAAGCTGTGTAGCCATTGCAAGAAAGAACTCGATATACCCCGAGAAACGTTGATTCAGGAAGGTTTTCCGGAGTCGAAA
ATTGGCACCTTCAAGATTTACGGTCCGGTCGGCTGTGAGCATTGCAACGGTGGCTACAGAGGAAGGGTCGGTATTTACGA
AGTGGTCAAAAAAACACCGGAACTGGAGCGCATCATCATGGAAGAAGGCAACTCGCTGGAGATATCCAGACAGATGCGCA
AGGACGGCTTCAATGACCTGCGTACCTCCGGCCTTTCAAAAGCCATGCAGGGCATAACCAGTCTTGAAGAAGTCAATCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.025

100

0.583
  pilB Acinetobacter baylyi ADP1

57.295

99.645

0.571
  pilB Legionella pneumophila strain ERS1305867

55.009

100

0.555
  pilB Vibrio cholerae strain A1552

50.89

99.645

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.272

97.695

0.491
  pilB Vibrio campbellii strain DS40M4

48.759

100

0.488
  pilF Neisseria gonorrhoeae MS11

48.043

99.645

0.479
  pilF Thermus thermophilus HB27

38.693

100

0.388