Detailed information NTG0441714

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HKJ33_RS06705
• Genome accession: NZ_CP052853
• Coordinates: 1452946..1454076 (+)
• Length: 376 a.a.
• NCBI ID: WP_004084950.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain RH1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1447946..1459076

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HKJ33_RS06685 (HKJ33_06825)	-	1448270..1449094 (+)	825	WP_004084943.1	glycosyltransferase family 2 protein	-
HKJ33_RS06690 (HKJ33_06830)	-	1449711..1450538 (-)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
HKJ33_RS06695 (HKJ33_06835)	-	1450923..1451135 (-)	213	WP_021358439.1	hypothetical protein	-
HKJ33_RS06700 (HKJ33_06840)	pilT	1451757..1452791 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
HKJ33_RS06705 (HKJ33_06845)	pilU	1452946..1454076 (+)	1131	WP_004084950.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HKJ33_RS06710 (HKJ33_06850)	-	1454770..1455738 (-)	969	WP_004084952.1	YafY family protein	-
HKJ33_RS06715 (HKJ33_06855)	-	1456521..1457330 (+)	810	WP_021358437.1	DUF481 domain-containing protein	-
HKJ33_RS06720 (HKJ33_06860)	hemC	1457384..1458301 (-)	918	WP_004084955.1	hydroxymethylbilane synthase	-
HKJ33_RS06725 (HKJ33_06865)	-	1458309..1459067 (-)	759	WP_004084957.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42402.63 Da
• Isoelectric Point: 6.3881

>NTDB_id=441714 HKJ33_RS06705 WP_004084950.1 1452946..1454076(+) (pilU) [Xylella fastidiosa subsp. multiplex strain RH1]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=441714 HKJ33_RS06705 WP_004084950.1 1452946..1454076(+) (pilU) [Xylella fastidiosa subsp. multiplex strain RH1]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402