Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HH212_RS03890 Genome accession   NZ_CP051685
Coordinates   904383..905558 (-) Length   391 a.a.
NCBI ID   WP_169434176.1    Uniprot ID   A0A7Z2VUD5
Organism   Massilia forsythiae strain GN2-R2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 899383..910558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH212_RS03865 (HH212_03865) - 900827..901732 (-) 906 WP_229217556.1 methyltransferase domain-containing protein -
  HH212_RS03870 (HH212_03870) - 901789..902565 (+) 777 WP_169434172.1 ComF family protein -
  HH212_RS03875 (HH212_03875) - 902643..903113 (+) 471 WP_169434173.1 tRNA (cytidine(34)-2'-O)-methyltransferase -
  HH212_RS03880 (HH212_03880) - 903270..903587 (+) 318 WP_169434174.1 H-NS family nucleoid-associated regulatory protein -
  HH212_RS03885 (HH212_03885) - 903769..904251 (-) 483 WP_169434175.1 adenylyltransferase/cytidyltransferase family protein -
  HH212_RS03890 (HH212_03890) pilU 904383..905558 (-) 1176 WP_169434176.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HH212_RS03895 (HH212_03895) - 905731..906681 (+) 951 WP_169434177.1 hydrogen peroxide-inducible genes activator -
  HH212_RS03900 (HH212_03900) ubiA 906900..907754 (+) 855 WP_169434178.1 4-hydroxybenzoate octaprenyltransferase -
  HH212_RS03905 (HH212_03905) - 907853..908173 (+) 321 WP_169434179.1 YqjD family protein -
  HH212_RS03910 (HH212_03910) - 908173..908598 (+) 426 WP_169434180.1 phage holin family protein -
  HH212_RS03915 (HH212_03915) - 908591..908908 (+) 318 WP_169434181.1 hypothetical protein -
  HH212_RS03920 (HH212_03920) proC 909051..909869 (-) 819 WP_169434182.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 43191.52 Da        Isoelectric Point: 6.5031

>NTDB_id=440452 HH212_RS03890 WP_169434176.1 904383..905558(-) (pilU) [Massilia forsythiae strain GN2-R2]
MSSTFGPAEAQAYMHKLLTVMQHQGGSDLFISADFPPSMKHQGAMKPLSNQRLTGEVTRALALSLMNERQRAEFEAEMEC
NFAISLPGVCRFRVNVFVQQQSVGMVVRTIASEIPNFEKLDLPEVLKDVVMTKRGLVLVVGGTGSGKSTTLAAMIDYRNA
NSAGHIITVEDPVEYVHKNKSCLITHREVGVDTLSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTMDRIINFFPEERRNQLLMDLSSNLRAIVSQRLVRTEDGKGRKAAIEILLNTPTIGEMILKGNFHGIKEIMHKS
RELGMCTFDQALFDLYNKGYIGYDEAIRNADSANGLRLQIKLSGERKAPGEGAVSKPAELSMLADEPEEGV

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=440452 HH212_RS03890 WP_169434176.1 904383..905558(-) (pilU) [Massilia forsythiae strain GN2-R2]
ATGTCTTCGACCTTCGGCCCGGCGGAAGCCCAGGCCTACATGCACAAGCTGCTGACCGTGATGCAGCACCAGGGCGGCTC
CGACCTGTTCATCTCCGCCGACTTCCCGCCCAGCATGAAGCACCAGGGCGCCATGAAGCCGCTCAGTAACCAGCGCCTGA
CCGGCGAGGTCACGCGCGCGCTGGCGTTGTCGCTGATGAACGAGCGCCAGCGCGCCGAGTTCGAGGCCGAGATGGAATGC
AATTTCGCCATCTCGCTGCCGGGCGTGTGCCGCTTCCGCGTCAACGTGTTCGTGCAGCAGCAGAGCGTGGGCATGGTGGT
GCGCACCATCGCCTCCGAAATCCCGAACTTCGAAAAGCTGGATTTGCCCGAGGTGTTGAAGGACGTGGTGATGACCAAGC
GCGGCCTGGTGCTGGTGGTGGGCGGCACCGGTTCCGGCAAGTCGACCACGCTGGCAGCGATGATCGACTATCGCAACGCC
AACTCCGCCGGCCACATCATCACGGTCGAGGACCCGGTCGAGTACGTCCACAAGAACAAGAGCTGCCTGATCACGCACCG
CGAGGTCGGCGTCGACACGCTGTCCTGGCACAACGCCCTGAAAAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCG
GCGAGATCCGCGACACCGAAACCATGGAGCACGCGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGGCACGCTGCAC
GCGAACAACGCCAACCAGACCATGGACCGCATCATCAACTTCTTCCCGGAAGAGCGAAGGAACCAGTTGCTGATGGACCT
GTCCTCGAACCTGCGCGCCATCGTCTCGCAGCGCCTGGTGCGCACCGAGGACGGCAAGGGCCGCAAGGCGGCGATCGAGA
TCCTGCTGAACACCCCGACCATCGGCGAGATGATCCTCAAGGGGAACTTCCACGGCATCAAGGAAATCATGCACAAGTCG
CGCGAACTCGGCATGTGCACCTTCGACCAGGCGCTGTTCGACCTGTACAACAAGGGCTACATCGGCTACGACGAAGCGAT
CCGCAATGCCGACTCCGCCAACGGCCTGCGCCTGCAGATCAAGCTCTCGGGCGAGCGCAAGGCGCCGGGCGAGGGCGCCG
TGTCGAAACCGGCCGAACTGTCGATGCTGGCGGACGAACCGGAAGAAGGGGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2VUD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.333

95.141

0.555
  pilU Acinetobacter baylyi ADP1

53.955

90.537

0.488
  pilU Vibrio cholerae strain A1552

50.142

90.281

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.012

85.422

0.376
  pilT Pseudomonas aeruginosa PAK

43.582

85.678

0.373
  pilT Pseudomonas stutzeri DSM 10701

42.985

85.678

0.368
  pilT Legionella pneumophila strain Lp02

43.731

83.632

0.366
  pilT Legionella pneumophila strain ERS1305867

43.731

83.632

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.333

84.399

0.366
  pilT Vibrio cholerae strain A1552

43.333

84.399

0.366
  pilT Acinetobacter baylyi ADP1

43.202

84.655

0.366