NTDB

Overview

Name	pilE	Type	Machinery gene
Locus tag	HBA48_RS09540	Genome accession	NZ_CP050135
Coordinates	1841012..1841485 (-)	Length	157 a.a.
NCBI ID	WP_164538010.1	Uniprot ID	-
Organism	Kingella kingae strain ATCC 23331
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1836012..1846485

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HBA48_RS09505 (HBA48_09545)	-	1836950..1837108 (+)	159	WP_164538012.1	hypothetical protein	-
HBA48_RS09510 (HBA48_09550)	-	1837111..1837836 (+)	726	WP_038302563.1	metal ABC transporter ATP-binding protein	-
HBA48_RS09515 (HBA48_09555)	-	1837885..1838277 (+)	393	WP_038302564.1	pyrimidine dimer DNA glycosylase/endonuclease V	-
HBA48_RS09520 (HBA48_09560)	-	1838313..1839203 (+)	891	WP_003788327.1	metal ABC transporter permease	-
HBA48_RS09530 (HBA48_09570)	-	1839649..1840425 (-)	777	WP_038302565.1	fimb protein	-
HBA48_RS09535 (HBA48_09575)	-	1840437..1840865 (-)	429	WP_019390096.1	pilin	-
HBA48_RS09540 (HBA48_09580)	pilE	1841012..1841485 (-)	474	WP_164538010.1	pilin	Machinery gene
HBA48_RS09545 (HBA48_09585)	recJ	1841845..1843557 (+)	1713	WP_038310302.1	single-stranded-DNA-specific exonuclease RecJ	-
HBA48_RS09550 (HBA48_09590)	-	1843774..1844508 (+)	735	WP_003791735.1	histidine phosphatase family protein	-
HBA48_RS09555 (HBA48_09595)	-	1844548..1845093 (+)	546	WP_038310304.1	gamma carbonic anhydrase family protein	-
HBA48_RS09560 (HBA48_09600)	-	1845084..1846133 (+)	1050	WP_038310306.1	metallophosphoesterase	-

Sequence

Protein

Download Length: 157 a.a. Molecular weight: 16343.50 Da Isoelectric Point: 9.0142

                                >NTDB_id=430274 HBA48_RS09540 WP_164538010.1 1841012..1841485(-) (pilE) [Kingella kingae strain ATCC 23331]

MKTMQKGFTLIELMIVIAIIGILAAIALPAYQDYTKRSRVSEGLSLAGGAKSALTEYYASNNEWPENNDKAGIAPANKIT

GNAVKSVEVARDTAGATGKGKITVTFNGKVKEGANLILEGTGNEAGSVQWTCTGGNLDAKFRPSECRNGTTAATNGS

Nucleotide

Download Length: 474 bp

                                >NTDB_id=430274 HBA48_RS09540 WP_164538010.1 1841012..1841485(-) (pilE) [Kingella kingae strain ATCC 23331]

ATGAAAACTATGCAAAAAGGTTTCACATTAATCGAGTTGATGATCGTAATCGCTATTATCGGTATTTTGGCTGCGATTGC

ATTGCCAGCTTACCAAGATTACACTAAACGTTCACGTGTATCTGAAGGTTTGAGCTTGGCTGGTGGTGCGAAAAGTGCTT

TGACTGAATACTACGCTTCTAACAATGAATGGCCTGAAAACAACGATAAAGCTGGTATTGCACCTGCTAATAAAATCACA

GGTAATGCAGTAAAAAGCGTAGAAGTTGCTCGTGATACTGCTGGTGCAACTGGTAAAGGTAAAATTACTGTTACATTTAA

CGGAAAAGTTAAAGAAGGCGCAAATTTGATTTTGGAAGGTACTGGTAACGAAGCTGGTTCGGTACAATGGACTTGTACTG

GTGGTAACTTGGATGCTAAATTCCGTCCTTCTGAATGCCGTAATGGTACTACTGCCGCTACCAATGGTAGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(37-147)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilE	Neisseria gonorrhoeae MS11	48.824	100	0.529
pilE	Neisseria gonorrhoeae strain FA1090	47.024	100	0.503
pilA2	Legionella pneumophila strain ERS1305867	50.694	91.72	0.465
pilA2	Legionella pneumophila str. Paris	50	91.72	0.459
pilA	Ralstonia pseudosolanacearum GMI1000	40.351	100	0.439
comP	Acinetobacter baylyi ADP1	46.259	93.631	0.433
pilA	Haemophilus influenzae 86-028NP	43.836	92.994	0.408
pilA	Acinetobacter baumannii strain A118	42.568	94.268	0.401
pilA/pilA1	Eikenella corrodens VA1	39.375	100	0.401
pilA	Haemophilus influenzae Rd KW20	42.857	93.631	0.401
pilA/pilAI	Pseudomonas stutzeri DSM 10701	40.667	95.541	0.389

Detailed information NTG0430274