Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   G7X26_RS08750 Genome accession   NZ_CP049775
Coordinates   1769468..1769743 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain ES-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1727081..1769444 1769468..1769743 flank 24


Gene organization within MGE regions


Location: 1727081..1769743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7X26_RS08455 (G7X26_08455) - 1727081..1728088 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  G7X26_RS08460 (G7X26_08460) comGG 1728217..1728570 (-) 354 WP_010825349.1 competence type IV pilus minor pilin ComGG -
  G7X26_RS08465 (G7X26_08465) comGF 1728570..1729004 (-) 435 WP_165548529.1 competence type IV pilus minor pilin ComGF -
  G7X26_RS08470 (G7X26_08470) - 1728994..1729365 (-) 372 WP_171025395.1 type II secretion system protein -
  G7X26_RS14690 - 1729699..1729824 (+) 126 WP_002364184.1 hypothetical protein -
  G7X26_RS08475 (G7X26_08475) hemH 1730121..1731062 (-) 942 WP_002364185.1 ferrochelatase -
  G7X26_RS08485 (G7X26_08485) - 1731802..1732050 (-) 249 WP_002383136.1 hypothetical protein -
  G7X26_RS08490 (G7X26_08490) - 1732122..1732322 (-) 201 WP_002357053.1 cold-shock protein -
  G7X26_RS08495 (G7X26_08495) - 1733159..1734400 (-) 1242 WP_165548531.1 LysM peptidoglycan-binding domain-containing protein -
  G7X26_RS08500 (G7X26_08500) - 1734401..1734634 (-) 234 WP_002384371.1 phage holin -
  G7X26_RS08505 (G7X26_08505) - 1734627..1734848 (-) 222 WP_002364191.1 hypothetical protein -
  G7X26_RS08510 (G7X26_08510) - 1734883..1735038 (-) 156 WP_002364192.1 XkdX family protein -
  G7X26_RS08515 (G7X26_08515) - 1735040..1735360 (-) 321 WP_002389262.1 hypothetical protein -
  G7X26_RS08520 (G7X26_08520) - 1735374..1735865 (-) 492 WP_002364194.1 hypothetical protein -
  G7X26_RS08525 (G7X26_08525) - 1735865..1736152 (-) 288 WP_002357046.1 collagen-like protein -
  G7X26_RS08530 (G7X26_08530) - 1736149..1736745 (-) 597 WP_002357045.1 hypothetical protein -
  G7X26_RS08535 (G7X26_08535) - 1736738..1737619 (-) 882 WP_104659692.1 phage baseplate upper protein -
  G7X26_RS08540 (G7X26_08540) - 1737638..1740445 (-) 2808 WP_165548533.1 phage tail spike protein -
  G7X26_RS08545 (G7X26_08545) - 1740427..1741161 (-) 735 WP_002403868.1 tail protein -
  G7X26_RS08550 (G7X26_08550) - 1741151..1744048 (-) 2898 WP_162782897.1 tape measure protein -
  G7X26_RS08555 (G7X26_08555) - 1744296..1744646 (-) 351 WP_002357039.1 hypothetical protein -
  G7X26_RS08560 (G7X26_08560) - 1744699..1745547 (-) 849 WP_165548535.1 major tail protein -
  G7X26_RS08565 (G7X26_08565) - 1745548..1745922 (-) 375 WP_002357037.1 DUF6838 family protein -
  G7X26_RS08570 (G7X26_08570) - 1745925..1746323 (-) 399 WP_002357036.1 HK97 gp10 family phage protein -
  G7X26_RS08575 (G7X26_08575) - 1746316..1746684 (-) 369 WP_002357034.1 hypothetical protein -
  G7X26_RS08580 (G7X26_08580) - 1746681..1747025 (-) 345 WP_002357033.1 hypothetical protein -
  G7X26_RS08585 (G7X26_08585) - 1747039..1747221 (-) 183 WP_002357032.1 hypothetical protein -
  G7X26_RS08590 (G7X26_08590) - 1747250..1748137 (-) 888 WP_165548537.1 DUF5309 domain-containing protein -
  G7X26_RS08595 (G7X26_08595) - 1748151..1748774 (-) 624 WP_165548539.1 DUF4355 domain-containing protein -
  G7X26_RS08600 (G7X26_08600) - 1748992..1749312 (-) 321 WP_002389265.1 hypothetical protein -
  G7X26_RS08605 (G7X26_08605) - 1749377..1749598 (-) 222 WP_002357027.1 hypothetical protein -
  G7X26_RS08610 (G7X26_08610) - 1749595..1751352 (-) 1758 WP_002384360.1 head protein -
  G7X26_RS08615 (G7X26_08615) - 1751327..1752814 (-) 1488 WP_002384358.1 phage portal protein -
  G7X26_RS08620 (G7X26_08620) - 1752826..1754115 (-) 1290 WP_002357024.1 PBSX family phage terminase large subunit -
  G7X26_RS08625 (G7X26_08625) terS 1754087..1754890 (-) 804 WP_002364203.1 phage terminase small subunit -
  G7X26_RS08630 (G7X26_08630) - 1754937..1755659 (-) 723 WP_104659696.1 DUF5677 domain-containing protein -
  G7X26_RS08635 (G7X26_08635) - 1756048..1756608 (-) 561 WP_104659697.1 hypothetical protein -
  G7X26_RS08640 (G7X26_08640) - 1756638..1757591 (-) 954 WP_104659698.1 DUF6731 family protein -
  G7X26_RS08650 (G7X26_08650) - 1758301..1758717 (-) 417 WP_002357018.1 ArpU family phage packaging/lysis transcriptional regulator -
  G7X26_RS08655 (G7X26_08655) - 1759624..1760058 (-) 435 WP_002402491.1 RusA family crossover junction endodeoxyribonuclease -
  G7X26_RS08660 (G7X26_08660) - 1760067..1760366 (-) 300 WP_002372047.1 MazG-like family protein -
  G7X26_RS08665 (G7X26_08665) - 1760367..1760669 (-) 303 WP_002368214.1 hypothetical protein -
  G7X26_RS08670 (G7X26_08670) - 1760666..1761526 (-) 861 WP_002402489.1 helix-turn-helix domain-containing protein -
  G7X26_RS08675 (G7X26_08675) - 1761526..1761726 (-) 201 WP_010715320.1 hypothetical protein -
  G7X26_RS08680 (G7X26_08680) - 1761731..1762372 (-) 642 WP_002402487.1 putative HNHc nuclease -
  G7X26_RS08685 (G7X26_08685) - 1762377..1763111 (-) 735 WP_165548541.1 ERF family protein -
  G7X26_RS08690 (G7X26_08690) - 1763104..1763421 (-) 318 WP_002402485.1 hypothetical protein -
  G7X26_RS08695 (G7X26_08695) - 1763617..1763955 (-) 339 WP_002402484.1 hypothetical protein -
  G7X26_RS08700 (G7X26_08700) - 1763992..1764201 (-) 210 WP_002378465.1 hypothetical protein -
  G7X26_RS08705 (G7X26_08705) - 1764256..1764444 (+) 189 WP_002357001.1 YegP family protein -
  G7X26_RS08710 (G7X26_08710) - 1764470..1765192 (-) 723 WP_165548543.1 ORF6C domain-containing protein -
  G7X26_RS08715 (G7X26_08715) - 1765215..1765526 (-) 312 WP_104659699.1 hypothetical protein -
  G7X26_RS08720 (G7X26_08720) - 1765538..1765729 (-) 192 WP_002383936.1 hypothetical protein -
  G7X26_RS08725 (G7X26_08725) - 1766025..1766366 (+) 342 WP_002387267.1 helix-turn-helix transcriptional regulator -
  G7X26_RS08730 (G7X26_08730) - 1766371..1767021 (+) 651 WP_002387266.1 ImmA/IrrE family metallo-endopeptidase -
  G7X26_RS08735 (G7X26_08735) - 1767117..1767746 (+) 630 WP_002410530.1 SHOCT domain-containing protein -
  G7X26_RS08740 (G7X26_08740) - 1767852..1769000 (+) 1149 WP_002387264.1 site-specific integrase -
  G7X26_RS08745 (G7X26_08745) comGD 1769037..1769471 (-) 435 Protein_1713 competence type IV pilus minor pilin ComGD -
  G7X26_RS08750 (G7X26_08750) comGC/cglC 1769468..1769743 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=427736 G7X26_RS08750 WP_002356991.1 1769468..1769743(-) (comGC/cglC) [Enterococcus faecalis strain ES-1]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=427736 G7X26_RS08750 WP_002356991.1 1769468..1769743(-) (comGC/cglC) [Enterococcus faecalis strain ES-1]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396