Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DSC_RS01330 Genome accession   NC_016147
Coordinates   305587..306723 (-) Length   378 a.a.
NCBI ID   WP_014159308.1    Uniprot ID   G7UVL3
Organism   Pseudoxanthomonas spadix BD-a59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 300587..311723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSC_RS01295 (DSC_02355) coq7 300991..301635 (-) 645 WP_014159302.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  DSC_RS01300 (DSC_02360) rplM 301869..302297 (+) 429 WP_014159303.1 50S ribosomal protein L13 -
  DSC_RS01305 (DSC_02365) rpsI 302300..302692 (+) 393 WP_014159304.1 30S ribosomal protein S9 -
  DSC_RS01315 (DSC_02370) - 303025..303762 (+) 738 WP_043290301.1 2OG-Fe dioxygenase family protein -
  DSC_RS01320 (DSC_02375) - 303824..304813 (+) 990 WP_014159306.1 fumarylacetoacetate hydrolase family protein -
  DSC_RS01325 (DSC_02380) maiA 304888..305565 (+) 678 WP_014159307.1 maleylacetoacetate isomerase -
  DSC_RS01330 (DSC_02385) pilU 305587..306723 (-) 1137 WP_014159308.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DSC_RS01335 (DSC_02390) - 306826..307278 (+) 453 WP_083833586.1 DUF4398 domain-containing protein -
  DSC_RS01340 (DSC_02395) - 307275..308162 (+) 888 WP_014159310.1 outer membrane protein -
  DSC_RS01345 (DSC_02400) - 308894..309112 (+) 219 WP_014159311.1 YdcH family protein -
  DSC_RS01350 (DSC_02405) - 309306..310682 (+) 1377 WP_014159312.1 pyridoxal-phosphate dependent enzyme -
  DSC_RS01355 (DSC_02410) - 310824..311072 (+) 249 WP_043290304.1 type II toxin-antitoxin system ParD family antitoxin -
  DSC_RS01360 (DSC_02415) - 311076..311393 (+) 318 WP_014159314.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41733.14 Da        Isoelectric Point: 6.2909

>NTDB_id=42729 DSC_RS01330 WP_014159308.1 305587..306723(-) (pilU) [Pseudoxanthomonas spadix BD-a59]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAVALAEAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELRLPQVLKDIIMTPRGLVLLVGSTGSGKSTSLASMIDFRNASTTGHILTIED
PIEYLHKHKQSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPETAHKNVLMNLALNLRAVVSQRLVKGVDGRRLPAAEVLINTPVIRDLLRRGQVHEIKQAMEESLEEGMESFDQCL
FRMVKEGKIVQEEALRAADSRDGLALKFRLSEGASGEHDPYADLYESGSPATITHGFG

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=42729 DSC_RS01330 WP_014159308.1 305587..306723(-) (pilU) [Pseudoxanthomonas spadix BD-a59]
ATGGACATCGGCTATTTCCTCAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACCACCGGCGCGCCCGTCTA
CATCAAGATCGAAGGCAAGCTCTATCCCCTGGGCAACACCGGCCTGCCACCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAACTGAACATGGCCGTGGCCCTGGCCGAGGCCGGACGT
TTCCGGGTGAATGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTCATCCGTGCCATCCGCAGCAAGATCCCCTCGATCGA
GGAACTGCGGCTGCCACAGGTGCTCAAGGACATCATCATGACCCCGCGCGGGCTGGTCCTGCTGGTCGGCTCGACCGGCT
CGGGCAAGTCCACCTCGCTGGCCTCGATGATCGACTTCCGCAACGCGAGCACCACCGGCCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACAAGCACAAGCAGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGATACCCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTGGACGCCACCACGATGGAGGCGG
CCATCGCCTTTGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGGATC
CTCAACTTCTTCCCCGAGACCGCGCACAAGAATGTACTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTCTCCCAGCG
CCTGGTCAAGGGCGTCGATGGCCGCCGCCTCCCGGCCGCCGAAGTGCTGATCAACACCCCGGTGATCCGTGACCTGCTGC
GTCGTGGCCAGGTCCACGAGATCAAGCAGGCGATGGAAGAATCGCTGGAAGAAGGCATGGAATCCTTCGACCAGTGCCTG
TTCCGCATGGTCAAGGAAGGCAAGATCGTGCAGGAGGAAGCGCTGCGCGCAGCCGACTCGCGCGACGGCCTGGCACTCAA
GTTCCGCCTGTCCGAGGGCGCCAGCGGCGAACACGATCCCTACGCGGACCTGTACGAAAGCGGTTCGCCGGCGACCATCA
CCCACGGCTTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G7UVL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.975

94.974

0.484
  pilU Vibrio cholerae strain A1552

47.027

97.884

0.46
  pilU Acinetobacter baylyi ADP1

48.739

94.444

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.194

100

0.437
  pilT Pseudomonas aeruginosa PAK

41.42

89.418

0.37
  pilT Acinetobacter baumannii D1279779

41.124

89.418

0.368
  pilT Acinetobacter nosocomialis M2

41.124

89.418

0.368
  pilT Acinetobacter baumannii strain A118

41.124

89.418

0.368
  pilT Pseudomonas stutzeri DSM 10701

40.533

89.418

0.362
  pilT Legionella pneumophila strain Lp02

40.533

89.418

0.362
  pilT Legionella pneumophila strain ERS1305867

40.533

89.418

0.362

Multiple sequence alignment