Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   G4Y78_RS31520 Genome accession   NZ_CP048878
Coordinates   1775206..1775709 (+) Length   167 a.a.
NCBI ID   WP_329604954.1    Uniprot ID   -
Organism   Spartinivicinus ruber strain S2-4-1H     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1770206..1780709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4Y78_RS08430 coaE 1770217..1770822 (-) 606 WP_163832602.1 dephospho-CoA kinase -
  G4Y78_RS08435 pilD 1770812..1771693 (-) 882 WP_163832603.1 A24 family peptidase Machinery gene
  G4Y78_RS08440 pilC 1771779..1772996 (-) 1218 WP_163832604.1 type II secretion system F family protein Machinery gene
  G4Y78_RS08445 pilB 1773242..1774936 (-) 1695 WP_163832605.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G4Y78_RS31520 pilA2 1775206..1775709 (+) 504 WP_329604954.1 pilin Machinery gene
  G4Y78_RS08455 - 1776602..1776978 (-) 377 Protein_1674 integrase core domain-containing protein -
  G4Y78_RS08460 - 1777160..1777392 (-) 233 Protein_1675 transposase -
  G4Y78_RS08465 - 1777578..1780487 (-) 2910 WP_329604955.1 fused MFS/spermidine synthase -

Sequence

Protein


Download         Length: 167 a.a.        Molecular weight: 17141.53 Da        Isoelectric Point: 6.7079

>NTDB_id=423727 G4Y78_RS31520 WP_329604954.1 1775206..1775709(+) (pilA2) [Spartinivicinus ruber strain S2-4-1H]
MESDYLILHTLNTTELGHMKHIKQQQQGFTLIELMIVVAIIGILAAVAIPAYQDYTVRSRVTEGLSLASSAKLAVAENAA
SANKSLSAGWTAPAATDNVTGLAVDATNGTITITYTAAAGGAGKTIILEPKSNGAALAAGTPPAGAVTWTCTGGDLEAKY
RPSNCRP

Nucleotide


Download         Length: 504 bp        

>NTDB_id=423727 G4Y78_RS31520 WP_329604954.1 1775206..1775709(+) (pilA2) [Spartinivicinus ruber strain S2-4-1H]
ATGGAAAGTGACTATTTAATTTTACATACCTTAAACACAACGGAGTTAGGTCACATGAAACACATTAAACAACAACAGCA
GGGTTTTACCCTTATTGAGTTAATGATTGTGGTTGCAATCATAGGAATATTGGCAGCAGTAGCGATACCTGCTTATCAAG
ACTATACAGTTAGATCAAGGGTTACAGAAGGCCTATCGCTTGCTTCTTCAGCAAAGTTGGCTGTAGCGGAAAATGCAGCA
TCAGCGAATAAAAGCTTATCAGCTGGTTGGACAGCACCTGCTGCAACTGACAATGTGACTGGTTTGGCTGTCGATGCAAC
CAATGGAACAATTACAATTACATATACTGCCGCAGCTGGTGGTGCTGGTAAGACTATAATTTTAGAGCCAAAGTCTAATG
GTGCTGCTCTTGCAGCAGGTACTCCACCTGCTGGTGCTGTGACCTGGACTTGTACAGGCGGTGATTTAGAAGCCAAATAT
AGACCTTCTAATTGTCGGCCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(57-166)

Prokaryotic N-terminal methylation motif (N_methyl)

(25-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

64.189

88.623

0.569
  pilA2 Legionella pneumophila str. Paris

63.514

88.623

0.563
  pilA Ralstonia pseudosolanacearum GMI1000

50.588

100

0.515
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40.526

100

0.461
  comP Acinetobacter baylyi ADP1

50.685

87.425

0.443
  pilE Neisseria gonorrhoeae MS11

37.576

98.802

0.371
  pilA Pseudomonas aeruginosa PAK

38.75

95.808

0.371
  pilA Acinetobacter baumannii strain A118

40.667

89.82

0.365
  pilA/pilA1 Eikenella corrodens VA1

36.527

100

0.365
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.397

90.419

0.365