Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LCGT_RS00725 Genome accession   NC_015930
Coordinates   145783..146727 (+) Length   314 a.a.
NCBI ID   WP_014024175.1    Uniprot ID   -
Organism   Lactococcus garvieae ATCC 49156     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 140783..151727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCGT_RS00710 (LCGT_0133) - 142193..143647 (-) 1455 WP_014024173.1 GH25 family lysozyme -
  LCGT_RS00715 (LCGT_0134) guaB 143816..145297 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  LCGT_RS00720 (LCGT_0135) rpsU 145420..145596 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  LCGT_RS00725 (LCGT_0136) comGA 145783..146727 (+) 945 WP_014024175.1 competence type IV pilus ATPase ComGA Machinery gene
  LCGT_RS00730 (LCGT_0137) comGB 146666..147685 (+) 1020 WP_016171027.1 competence type IV pilus assembly protein ComGB Machinery gene
  LCGT_RS00735 (LCGT_0138) comYC 147704..148009 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  LCGT_RS00740 (LCGT_0139) comGD 147984..148400 (+) 417 WP_014024177.1 competence type IV pilus minor pilin ComGD Machinery gene
  LCGT_RS00745 (LCGT_0140) comGE 148432..148665 (+) 234 WP_231844109.1 competence type IV pilus minor pilin ComGE -
  LCGT_RS00750 (LCGT_0141) comYF 148646..149086 (+) 441 WP_014024179.1 competence type IV pilus minor pilin ComGF Machinery gene
  LCGT_RS00755 (LCGT_0143) - 149319..150164 (+) 846 WP_014024181.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LCGT_RS00760 (LCGT_0144) - 150283..151119 (-) 837 WP_014024182.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35851.08 Da        Isoelectric Point: 6.8344

>NTDB_id=42078 LCGT_RS00725 WP_014024175.1 145783..146727(+) (comGA) [Lactococcus garvieae ATCC 49156]
MIQVLAKTLLQKAVDQMVHDLYFVALEGKYSLYFRTATERRFERELDLEQGQALIAHMKFLSGMNLGESRRVQLGACTYV
LDKGEQRLRLSTVGNFHGQESLVIRLLHHQQNQLHFWNSEIFKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVISR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAHEKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=42078 LCGT_RS00725 WP_014024175.1 145783..146727(+) (comGA) [Lactococcus garvieae ATCC 49156]
ATGATACAGGTTTTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTATTTCGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCGATCTTGAACAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTT
TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTAATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATTTTTAAATCTTTTATTGGTGGGAGAGGGCTTTACCTGT
TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTTATGTACAAGTTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA
TGCCTTGATTAAGCTCTCTCTTCGCCACAGGCCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAAACGGCAAAAG
CTGTTCTTCGCGCAAGTTTGACGGGATATACAGTGTTTTCTACTGTTCATGCGCGCTCAATATCGGGTGTAATTTCACGG
CTAAAGGAACTTGGATTAACTGATTGGGAGCTACAATCCAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA
GGGGTTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGATGAATGGAACGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.236

100

0.592
  comYA Streptococcus gordonii str. Challis substr. CH1

53.333

100

0.535
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.716

99.682

0.525
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

51.757

99.682

0.516
  comYA Streptococcus mutans UA159

50.962

99.363

0.506
  comYA Streptococcus mutans UA140

50.962

99.363

0.506

Multiple sequence alignment