Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LCGT_RS00730 Genome accession   NC_015930
Coordinates   146666..147685 (+) Length   339 a.a.
NCBI ID   WP_016171027.1    Uniprot ID   -
Organism   Lactococcus garvieae ATCC 49156     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 141666..152685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCGT_RS00710 (LCGT_0133) - 142193..143647 (-) 1455 WP_014024173.1 GH25 family lysozyme -
  LCGT_RS00715 (LCGT_0134) guaB 143816..145297 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  LCGT_RS00720 (LCGT_0135) rpsU 145420..145596 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  LCGT_RS00725 (LCGT_0136) comGA 145783..146727 (+) 945 WP_014024175.1 competence type IV pilus ATPase ComGA Machinery gene
  LCGT_RS00730 (LCGT_0137) comGB 146666..147685 (+) 1020 WP_016171027.1 competence type IV pilus assembly protein ComGB Machinery gene
  LCGT_RS00735 (LCGT_0138) comYC 147704..148009 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  LCGT_RS00740 (LCGT_0139) comGD 147984..148400 (+) 417 WP_014024177.1 competence type IV pilus minor pilin ComGD Machinery gene
  LCGT_RS00745 (LCGT_0140) comGE 148432..148665 (+) 234 WP_231844109.1 competence type IV pilus minor pilin ComGE -
  LCGT_RS00750 (LCGT_0141) comYF 148646..149086 (+) 441 WP_014024179.1 competence type IV pilus minor pilin ComGF Machinery gene
  LCGT_RS00755 (LCGT_0143) - 149319..150164 (+) 846 WP_014024181.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LCGT_RS00760 (LCGT_0144) - 150283..151119 (-) 837 WP_014024182.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39294.00 Da        Isoelectric Point: 9.8252

>NTDB_id=42079 LCGT_RS00730 WP_016171027.1 146666..147685(+) (comGB) [Lactococcus garvieae ATCC 49156]
MDLSQLLQLRTKKLSLAKQIKLVQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMRAGLLNGQSFSGILAKLKFNEDI
VTQLSLAESHGNVEYTLGLIEEKLRRVLNIRKKLIQVATYPLVLLAFLIFIMLGLKNYLLPQLDENRGLATYVIQNLPSL
FLGCLFSLLVVFYLGRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLHILDRGQKFEDALRLYPIFTKELALIVEYGELKSKLGKELMVFSEESWALFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=42079 LCGT_RS00730 WP_016171027.1 146666..147685(+) (comGB) [Lactococcus garvieae ATCC 49156]
ATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAAGCTTGTTCAACTTATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTTTCTGAAATTATTGATTTTCTTGAACGGTCGGGTCTCACGGAAGCATACTTTA
TTTCCAAAATGCGTGCAGGTTTACTCAACGGGCAAAGTTTCTCTGGTATTTTAGCGAAGCTCAAATTTAATGAGGATATT
GTGACTCAGCTCTCTTTGGCCGAGTCTCATGGGAATGTTGAGTATACTTTAGGGTTGATTGAAGAAAAACTGAGACGCGT
TTTGAACATTCGCAAAAAGCTCATCCAAGTTGCCACTTACCCGCTCGTTCTTCTGGCATTTCTAATCTTTATCATGCTAG
GGTTGAAAAATTATTTACTGCCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGTTATACAGAATCTTCCGTCCCTC
TTTTTAGGGTGCCTCTTCAGTTTGCTTGTTGTCTTTTATCTGGGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTAATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCTCAAGCAGTCGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTTTTGAGCTCCTTCATATATTAGATAGGGGTCAGAAGTTTGAAGATGCCCTTCGACTTTACCCCATATTTACCAA
GGAGTTGGCATTAATTGTGGAATATGGTGAACTAAAGTCCAAGCTAGGGAAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGGCGCTCTTTTTTGAAAGAGTTGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCTGCAATGTTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

53.846

99.705

0.537
  comGB/cglB Streptococcus mitis NCTC 12261

53.433

98.82

0.528
  comGB/cglB Streptococcus pneumoniae D39

52.239

98.82

0.516
  comGB/cglB Streptococcus pneumoniae R6

52.239

98.82

0.516
  comGB/cglB Streptococcus pneumoniae TIGR4

52.239

98.82

0.516
  comGB/cglB Streptococcus mitis SK321

52.239

98.82

0.516
  comGB/cglB Streptococcus pneumoniae Rx1

52.239

98.82

0.516
  comYB Streptococcus gordonii str. Challis substr. CH1

51.488

99.115

0.51
  comYB Streptococcus mutans UA140

51.429

92.92

0.478
  comYB Streptococcus mutans UA159

51.111

92.92

0.475

Multiple sequence alignment