Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GVI47_RS23390 Genome accession   NZ_CP047698
Coordinates   5001320..5002537 (+) Length   405 a.a.
NCBI ID   WP_160288285.1    Uniprot ID   -
Organism   Pseudomonas knackmussii strain N1-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4996320..5007537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVI47_RS23375 - 4997172..4998893 (-) 1722 WP_160288282.1 phosphoethanolamine transferase -
  GVI47_RS23380 comP 4998954..4999385 (-) 432 WP_160288283.1 pilin Machinery gene
  GVI47_RS23385 pilB 4999613..5001316 (+) 1704 WP_160288284.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GVI47_RS23390 pilC 5001320..5002537 (+) 1218 WP_160288285.1 type II secretion system F family protein Machinery gene
  GVI47_RS23395 pilD 5002540..5003412 (+) 873 WP_160288286.1 A24 family peptidase Machinery gene
  GVI47_RS23400 coaE 5003498..5004106 (+) 609 WP_160288287.1 dephospho-CoA kinase -
  GVI47_RS23405 yacG 5004103..5004312 (+) 210 WP_160288288.1 DNA gyrase inhibitor YacG -
  GVI47_RS23410 - 5004490..5004705 (-) 216 WP_160288289.1 hypothetical protein -
  GVI47_RS23415 - 5004813..5005532 (-) 720 WP_160288290.1 energy-coupling factor ABC transporter permease -
  GVI47_RS23420 - 5005529..5005996 (-) 468 WP_236658330.1 FAD/FMN-containing dehydrogenase -
  GVI47_RS23425 - 5006000..5006416 (-) 417 WP_160288291.1 GNAT family N-acetyltransferase -
  GVI47_RS23430 - 5006514..5007143 (+) 630 WP_160288292.1 DUF1780 domain-containing protein -
  GVI47_RS23435 - 5007213..5007386 (+) 174 WP_043254973.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44243.10 Da        Isoelectric Point: 9.9941

>NTDB_id=415462 GVI47_RS23390 WP_160288285.1 5001320..5002537(+) (pilC) [Pseudomonas knackmussii strain N1-2]
MAEKALKTSVFTFEGTDRKGGKIKGELSGTNTALVKAQLRKQGINPTKVRKKGMSLLSKGKKIKPMDIALFTRQMATMMQ
SGVPLLQSFDIIGEGFDNPNMKKLVEEIKQEVAAGNSLANSLRKKPLYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKGKIKKAMTYPIAVVVVAIVVSAILLIKVVPQFQSVFANFGAELPAFTQMVINLSEILQKWWLIVLIAMFAIAFSLK
EYHRRSEKFRNSVDRGLLKLPIVGMILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRNAVGKIKQDVSSGT
QLNFSMRTTGVFPNMAIQMTAIGEEAGSLDAMLDKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=415462 GVI47_RS23390 WP_160288285.1 5001320..5002537(+) (pilC) [Pseudomonas knackmussii strain N1-2]
ATGGCGGAAAAAGCGTTGAAAACCAGTGTCTTCACCTTCGAGGGCACGGACCGCAAGGGCGGGAAGATTAAAGGGGAGCT
ATCGGGCACCAACACCGCCCTGGTCAAGGCGCAGTTGCGCAAGCAGGGTATCAACCCGACCAAGGTACGCAAGAAGGGCA
TGTCCCTGTTATCCAAGGGCAAGAAGATCAAGCCCATGGACATTGCCCTGTTCACCCGACAGATGGCGACCATGATGCAG
TCCGGCGTTCCGCTGCTCCAATCATTCGACATTATTGGGGAAGGCTTCGACAATCCGAACATGAAGAAGCTGGTCGAGGA
GATCAAGCAGGAGGTGGCTGCCGGTAACAGCCTTGCTAACTCCTTGCGCAAGAAGCCGCTGTATTTCGATGATCTCTATT
GCAATCTTGTGGATGCCGGCGAGCAGTCCGGTGCGCTGGAAACGCTGCTGGACCGCGTGGCTACTTACAAGGAAAAGACT
GAATCCCTGAAAGGCAAAATCAAGAAAGCGATGACTTATCCCATTGCGGTCGTCGTAGTCGCGATAGTGGTTTCTGCCAT
TCTATTAATCAAGGTGGTTCCTCAGTTCCAATCAGTATTTGCCAACTTCGGCGCCGAGCTTCCTGCATTTACGCAAATGG
TAATCAATCTCTCAGAAATCCTTCAAAAATGGTGGCTTATCGTTTTAATTGCAATGTTTGCCATCGCATTTTCCTTGAAG
GAATATCATCGGCGCTCTGAGAAATTCCGGAACTCTGTAGATCGTGGGCTTCTGAAACTTCCTATTGTTGGAATGATCCT
CTATAAATCAGCAGTAGCCCGCTACGCACGCACGCTCTCAACGACATTCGCAGCTGGCGTACCCCTGGTAGAGGCACTTG
ACTCTGTATCAGGCGCGACGGGCAACGTCGTATTTCGCAACGCCGTAGGCAAAATCAAGCAGGACGTCTCATCCGGGACA
CAGCTGAACTTCTCGATGCGAACAACAGGCGTTTTCCCCAATATGGCTATACAGATGACGGCCATTGGTGAGGAGGCAGG
TTCGCTGGATGCAATGCTCGACAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTCGACAACCTCACAACCC
TGATGGAACCCATGATCATGGCCGTACTAGGTGTGCTCGTTGGCGGCCTGATCATTGCCATGTACCTCCCGATCTTCCAA
CTAGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.506

100

0.795
  pilC Acinetobacter baumannii D1279779

59.753

100

0.598
  pilC Acinetobacter baylyi ADP1

58.621

100

0.588
  pilC Legionella pneumophila strain ERS1305867

55.051

97.778

0.538
  pilG Neisseria meningitidis 44/76-A

46.65

99.506

0.464
  pilG Neisseria gonorrhoeae MS11

45.906

99.506

0.457
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

37.157

99.012

0.368

Multiple sequence alignment