Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GVI47_RS23385 Genome accession   NZ_CP047698
Coordinates   4999613..5001316 (+) Length   567 a.a.
NCBI ID   WP_160288284.1    Uniprot ID   -
Organism   Pseudomonas knackmussii strain N1-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4994613..5006316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVI47_RS23360 nadC 4994953..4995801 (+) 849 WP_160288280.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GVI47_RS23365 - 4995802..4996872 (-) 1071 WP_236658329.1 diguanylate cyclase -
  GVI47_RS23375 - 4997172..4998893 (-) 1722 WP_160288282.1 phosphoethanolamine transferase -
  GVI47_RS23380 comP 4998954..4999385 (-) 432 WP_160288283.1 pilin Machinery gene
  GVI47_RS23385 pilB 4999613..5001316 (+) 1704 WP_160288284.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GVI47_RS23390 pilC 5001320..5002537 (+) 1218 WP_160288285.1 type II secretion system F family protein Machinery gene
  GVI47_RS23395 pilD 5002540..5003412 (+) 873 WP_160288286.1 A24 family peptidase Machinery gene
  GVI47_RS23400 coaE 5003498..5004106 (+) 609 WP_160288287.1 dephospho-CoA kinase -
  GVI47_RS23405 yacG 5004103..5004312 (+) 210 WP_160288288.1 DNA gyrase inhibitor YacG -
  GVI47_RS23410 - 5004490..5004705 (-) 216 WP_160288289.1 hypothetical protein -
  GVI47_RS23415 - 5004813..5005532 (-) 720 WP_160288290.1 energy-coupling factor ABC transporter permease -
  GVI47_RS23420 - 5005529..5005996 (-) 468 WP_236658330.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62654.70 Da        Isoelectric Point: 5.4651

>NTDB_id=415461 GVI47_RS23385 WP_160288284.1 4999613..5001316(+) (pilB) [Pseudomonas knackmussii strain N1-2]
MNDQVVLSGLARQLVIAELLDEKSAQQAQQQAQRNKLSLVTYLVQNKLVKSRPLTELAADQFGIAYFDLGSIDRESIPKE
LVSEKLVRQHRVIPLWRRGNKLFVGLSDPTNHQAVTDVQFSTGLTTEAILVEDDKLGDAIEKLFESVTGGLDDLGDVDLE
GLDIESGNAERKEEAIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEDYQKQLYLEALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKQGMDFSTALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSVNLIIAQRLARKLCSTCKKEHDVPREALLHEGFP
EDRIGNFKLYSPVGCENCNGGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=415461 GVI47_RS23385 WP_160288284.1 4999613..5001316(+) (pilB) [Pseudomonas knackmussii strain N1-2]
ATGAACGATCAAGTCGTCCTCTCCGGCCTCGCCCGCCAGTTGGTGATTGCCGAGCTGCTCGATGAGAAGAGCGCGCAACA
GGCTCAGCAACAGGCGCAGCGCAACAAGCTATCGCTGGTGACCTACCTGGTGCAGAACAAGCTGGTCAAGAGCCGGCCGC
TGACCGAACTGGCTGCCGACCAGTTCGGCATTGCCTATTTCGATCTGGGCAGTATCGATCGAGAGAGCATTCCCAAGGAA
CTGGTAAGCGAAAAGCTGGTGCGACAACACCGGGTCATTCCGCTCTGGCGCCGAGGGAACAAGCTGTTCGTGGGACTTTC
CGATCCCACCAACCACCAGGCCGTGACCGATGTGCAATTCAGCACCGGTCTGACCACCGAAGCGATCCTGGTTGAGGACG
ACAAACTCGGTGATGCAATCGAAAAGCTATTCGAAAGCGTCACAGGCGGATTGGATGATCTCGGCGATGTCGATCTAGAA
GGGCTAGATATCGAGAGCGGGAACGCCGAGCGCAAAGAAGAAGCAATTGGCGGCGACGCGGATGACGCCCCAGTTGTGCG
CTTTGTCAACAAAATGCTGTTGGACGCCATTAAGGGCGGCTCCTCCGACCTGCACTTTGAACCCTACGAGAAAATTTACC
GCGTACGCTTCCGTACCGACGGCATGCTGCACGAAGTAGCCAAGCCCCCCATCCAATTGGCCAGCCGTATCTCAGCGCGC
CTTAAGGTCATGGCCGGGCTGGACATTTCCGAGCGCCGCAAGCCGCAGGACGGCCGCATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGTGTCAACACCTTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGATTCGGCCA
GCGCGCAAATGGGCATCGACGCCCTTGGCTACGAGGACTACCAGAAGCAACTCTATCTGGAGGCGCTGAAACAGCCGCAA
GGCATGATCCTGGTTACCGGCCCGACCGGCTCGGGCAAGACGGTTTCCCTGTATACCGGCCTGAATATCCTCAATACCCC
CGACATCAACATTTCCACTGCCGAAGACCCGGTTGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTGAACCCCAAGC
AGGGCATGGATTTCTCCACGGCCTTGCGCGCGTTTCTACGACAGGACCCGGATGTAATCATGGTCGGCGAGATCCGCGAC
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCGCAGACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACCCTGACCCGACTGCTGAATATGGGCGTGGCCTCGTTCAACCTGGCTACCTCGGTCAACCTGATCATTGCCCAGC
GCCTGGCGCGCAAACTCTGCTCCACCTGCAAGAAGGAGCACGATGTTCCGCGCGAAGCCCTGCTCCACGAAGGCTTCCCG
GAAGACAGGATCGGCAACTTCAAGCTCTACTCGCCGGTGGGATGTGAAAATTGCAATGGCGGCTACAAAGGTCGTGTGGG
TATTTATGAAGTGGTTAAAATCACGCCGGCGCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATTCAGATCGCCGAAC
AAGCCCGCAAAGAAGGCTTCAATGATCTGCGCACCTCGGGCCTGCTGAAGGCCATGCAAGGCATCACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.761

99.647

0.586
  pilB Acinetobacter baylyi ADP1

58.333

99.471

0.58
  pilB Legionella pneumophila strain ERS1305867

55.203

100

0.552
  pilF Neisseria gonorrhoeae MS11

50.441

100

0.504
  pilB Vibrio cholerae strain A1552

50.799

99.295

0.504
  pilB Vibrio campbellii strain DS40M4

49.823

99.471

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.292

95.591

0.49
  pilF Thermus thermophilus HB27

38.772

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.301

93.651

0.377

Multiple sequence alignment