Detailed information NTG0415463

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GVI47_RS23395
• Genome accession: NZ_CP047698
• Coordinates: 5002540..5003412 (+)
• Length: 290 a.a.
• NCBI ID: WP_160288286.1
• Uniprot ID: -
• Organism: Pseudomonas knackmussii strain N1-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4997540..5008412

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GVI47_RS23380	comP	4998954..4999385 (-)	432	WP_160288283.1	pilin	Machinery gene
GVI47_RS23385	pilB	4999613..5001316 (+)	1704	WP_160288284.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GVI47_RS23390	pilC	5001320..5002537 (+)	1218	WP_160288285.1	type II secretion system F family protein	Machinery gene
GVI47_RS23395	pilD	5002540..5003412 (+)	873	WP_160288286.1	A24 family peptidase	Machinery gene
GVI47_RS23400	coaE	5003498..5004106 (+)	609	WP_160288287.1	dephospho-CoA kinase	-
GVI47_RS23405	yacG	5004103..5004312 (+)	210	WP_160288288.1	DNA gyrase inhibitor YacG	-
GVI47_RS23410	-	5004490..5004705 (-)	216	WP_160288289.1	hypothetical protein	-
GVI47_RS23415	-	5004813..5005532 (-)	720	WP_160288290.1	energy-coupling factor ABC transporter permease	-
GVI47_RS23420	-	5005529..5005996 (-)	468	WP_236658330.1	FAD/FMN-containing dehydrogenase	-
GVI47_RS23425	-	5006000..5006416 (-)	417	WP_160288291.1	GNAT family N-acetyltransferase	-
GVI47_RS23430	-	5006514..5007143 (+)	630	WP_160288292.1	DUF1780 domain-containing protein	-
GVI47_RS23435	-	5007213..5007386 (+)	174	WP_043254973.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31967.85 Da
• Isoelectric Point: 6.7555

>NTDB_id=415463 GVI47_RS23395 WP_160288286.1 5002540..5003412(+) (pilD) [Pseudomonas knackmussii strain N1-2]
MPVVDFLASHALAFVLCAALLGLLVGSFLNVVIHRLPKMLQLDWEQQARDTLALPHPEKHETYNLILPNSHCPHCDHEIR
PWENIPVVSYLALRGKCSSCKAPISARYPLIEVATALLSGFVAWHFGFTWQAGAMLLLSWGLLAMSMIDVDHQLLPDALV
LPLLWLGLIVNAFGLFTSLSDALWGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSLVG
AVLGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITTSYLQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=415463 GVI47_RS23395 WP_160288286.1 5002540..5003412(+) (pilD) [Pseudomonas knackmussii strain N1-2]
ATGCCTGTCGTCGATTTCCTGGCCAGCCACGCGCTGGCCTTTGTTTTGTGCGCTGCCCTACTCGGCCTGTTGGTCGGCAG
CTTCCTCAACGTGGTTATTCACCGCCTCCCAAAGATGCTGCAACTCGACTGGGAACAGCAGGCCAGAGACACGCTCGCTC
TTCCTCATCCCGAAAAGCACGAAACCTACAACCTCATCCTGCCCAACTCCCACTGCCCGCATTGCGACCATGAGATTCGC
CCCTGGGAGAACATCCCGGTGGTGAGCTACCTGGCGCTGCGCGGCAAGTGTTCGTCCTGCAAGGCGCCGATCAGTGCGCG
TTATCCGCTGATCGAGGTGGCCACCGCCCTCCTCTCGGGTTTCGTTGCCTGGCACTTCGGCTTCACCTGGCAGGCGGGCG
CGATGTTGCTGCTGAGCTGGGGGCTTCTGGCGATGAGCATGATCGATGTCGATCACCAGTTGCTGCCCGATGCGCTGGTG
CTTCCGCTGCTCTGGCTCGGTCTGATCGTCAATGCTTTCGGCCTCTTCACCTCGCTGAGCGATGCGCTCTGGGGCGCGGT
TTTCGGCTACCTCAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTCACCGGCAAGGAAGGCATGGGCTATGGCGACT
TCAAGCTGCTGGCCATGCTCGGCGCCTGGGGTGGCTGGCAGGTGCTGCCGCTGACCATCCTGCTTTCATCGCTGGTTGGG
GCGGTGCTAGGCGTTATCATGCTGCGCCTGCGCAATGCCGAAAGCGGCACGCCCATCCCCTTCGGGCCCTACCTGGCCAT
TGCCGGCTGGATTGCGCTGCTCTGGGGTGATCAAATAACCACTTCCTATCTGCAGTTCGCCGGATTCCGCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	56.727	94.828	0.538
pilD	Vibrio campbellii strain DS40M4	54.579	94.138	0.514
pilD	Acinetobacter baumannii D1279779	52.297	97.586	0.51
pilD	Acinetobacter nosocomialis M2	51.59	97.586	0.503
pilD	Neisseria gonorrhoeae MS11	50.19	90.69	0.455