Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   I5102_RS25250 Genome accession   NZ_CP060240
Coordinates   5380591..5381055 (-) Length   154 a.a.
NCBI ID   WP_023086642.1    Uniprot ID   Q8KQ34
Organism   Pseudomonas aeruginosa strain G-I-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5375591..5386055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5102_RS25235 (I5102_005177) nadC 5378091..5378939 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I5102_RS25245 (I5102_005179) - 5379120..5380433 (-) 1314 WP_223218619.1 O-antigen ligase family protein -
  I5102_RS25250 (I5102_005180) pilA/pilAI 5380591..5381055 (-) 465 WP_023086642.1 pilin Machinery gene
  I5102_RS25255 (I5102_005181) pilB 5381286..5382986 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I5102_RS25260 (I5102_005182) pilC 5382990..5384207 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  I5102_RS25265 (I5102_005183) pilD 5384208..5385080 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  I5102_RS25270 (I5102_005184) coaE 5385077..5385688 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  I5102_RS25275 (I5102_005185) yacG 5385685..5385885 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16021.32 Da        Isoelectric Point: 8.9987

>NTDB_id=413722 I5102_RS25250 WP_023086642.1 5380591..5381055(-) (pilA/pilAI) [Pseudomonas aeruginosa strain G-I-1]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGSGKGQIKIDKADTATPEISGTLGGSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=413722 I5102_RS25250 WP_023086642.1 5380591..5381055(-) (pilA/pilAI) [Pseudomonas aeruginosa strain G-I-1]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTAGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACTGGTAGCGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCGGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8KQ34

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.161

100

0.455
  pilA Acinetobacter baumannii strain A118

46.207

94.156

0.435
  pilA Pseudomonas aeruginosa PAK

41.772

100

0.429
  pilA Ralstonia pseudosolanacearum GMI1000

36.471

100

0.403
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.073

98.052

0.383
  pilA Vibrio cholerae strain A1552

39.073

98.052

0.383
  pilA Vibrio cholerae C6706

39.073

98.052

0.383
  pilA2 Legionella pneumophila strain ERS1305867

38.514

96.104

0.37
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.255

96.753

0.37
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364