Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GST86_RS26805 Genome accession   NZ_CP047148
Coordinates   5797486..5798748 (-) Length   420 a.a.
NCBI ID   WP_010955626.1    Uniprot ID   -
Organism   Pseudomonas putida strain PP2323     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5792486..5803748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GST86_RS26790 (GST86_26460) - 5794164..5795753 (-) 1590 WP_010955623.1 SPOR domain-containing protein -
  GST86_RS26795 (GST86_26465) aroB 5795769..5796866 (-) 1098 WP_010955624.1 3-dehydroquinate synthase -
  GST86_RS26800 (GST86_26470) aroK 5796963..5797481 (-) 519 WP_010955625.1 shikimate kinase AroK -
  GST86_RS26805 (GST86_26475) pilQ 5797486..5798748 (-) 1263 WP_010955626.1 type IV pilus secretin PilQ Machinery gene
  GST86_RS26810 (GST86_26480) - 5798672..5799673 (-) 1002 WP_049588306.1 pilus assembly protein PilP -
  GST86_RS26815 (GST86_26485) - 5799685..5800221 (-) 537 WP_010955628.1 PilN domain-containing protein -
  GST86_RS26820 (GST86_26490) pilM 5800218..5801099 (-) 882 WP_010955629.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45687.40 Da        Isoelectric Point: 5.0856

>NTDB_id=411020 GST86_RS26805 WP_010955626.1 5797486..5798748(-) (pilQ) [Pseudomonas putida strain PP2323]
MKTWMKYLLATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGSVTLRLQDVPWDQALDLVLR
SKGLSRRQEGNVLLVAPAAEFAAQSADARVSQALDAHLQPLRRELLPIHHAKAADLAQLLLAGLEGDGIPPASLSVDERT
NTLVVHLPADRLAEMRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNKV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=411020 GST86_RS26805 WP_010955626.1 5797486..5798748(-) (pilQ) [Pseudomonas putida strain PP2323]
ATGAAAACGTGGATGAAATACCTGTTGGCGACCCTGCTGTCGATCCCGCTGGCTTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGCTGAACTTCCAGGATGTTGAGGTGCGTGCGGTGCTGCAGGTGCTGGCTGACTACGCTGGCGTCAACCTGGTCG
CCAGTGATGCGGTACAAGGCAGTGTCACTTTGCGTTTGCAGGACGTGCCGTGGGATCAGGCGCTTGACCTGGTCCTGCGC
AGCAAGGGCCTGTCCCGGCGCCAGGAGGGCAATGTGCTGTTGGTGGCACCTGCAGCCGAGTTTGCCGCGCAATCAGCAGA
CGCCCGGGTGAGTCAGGCGCTGGATGCGCACCTGCAGCCATTGCGTCGGGAGCTGCTGCCCATTCACCACGCCAAGGCCG
CCGATCTGGCGCAGTTGCTGCTGGCAGGCCTGGAGGGTGACGGCATCCCTCCGGCTAGCTTGAGTGTCGATGAGCGCACC
AACACGTTGGTGGTGCATCTGCCCGCTGACCGCCTGGCCGAGATGCGCCAGCTGGTGGCGCAGTTGGATGTGCCGGTGCG
CCAGGTAGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTATGAGAAAAGCCTGGGGGTACGCTGGGGTGGGCCGC
TGTACGGCGAGAAACTGCGGCCCGGCAAGGAGCTGTTCGTCGACCTCGGTCTGGAGCGTGCCGGCAGCAGTATCGGCCTG
GGTTTGTTGCGCGGTGACGTGCTGCTGGACCTGGAGCTCAGTGCCATGGAAAAAAGCGGCAACGGCGAAATCATTTCGCA
GCCCAAGGTGGTCACGGCTGACAAGGAAACGGCGAGGATCCTCAAGGGTACCGAGGTGCCTTACCAGGAAACGAGCCAAA
GCGGCGCTACCTCGGTATCGTTTCGCGAAGCCTCGCTGTCGCTGGAAGTAACGCCACAGATCACCCCGGACAACAAGGTG
ATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATTCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTCTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.174

100

0.614
  comQ Acinetobacter baylyi ADP1

42.539

100

0.455
  pilQ Acinetobacter baumannii strain A118

41.215

100

0.452
  pilQ Acinetobacter baumannii D1279779

41.215

100

0.452
  pilQ Legionella pneumophila strain ERS1305867

43.029

99.048

0.426
  pilQ Legionella pneumophila strain Lp02

43.029

99.048

0.426
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.943

100

0.421
  pilQ Vibrio cholerae strain A1552

41.943

100

0.421
  pilQ Vibrio campbellii strain DS40M4

40.46

100

0.419
  pilQ Neisseria gonorrhoeae strain FA1090

35.765

100

0.362
  pilQ Neisseria meningitidis MC58

35.765

100

0.362
  pilQ Neisseria meningitidis M1080

35.765

100

0.362

Multiple sequence alignment