Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPY13_RS13230 Genome accession   NZ_CP046823
Coordinates   2870313..2872028 (-) Length   571 a.a.
NCBI ID   WP_032470621.1    Uniprot ID   -
Organism   Vibrio mimicus strain 06-2455     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2865313..2877028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY13_RS13210 - 2866063..2866896 (-) 834 WP_000744685.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  GPY13_RS13215 - 2866966..2868435 (-) 1470 WP_000050203.1 AAA family ATPase -
  GPY13_RS13220 aroB 2868489..2869574 (-) 1086 WP_000439800.1 3-dehydroquinate synthase -
  GPY13_RS13225 aroK 2869602..2870126 (-) 525 WP_000818614.1 shikimate kinase AroK -
  GPY13_RS13230 pilQ 2870313..2872028 (-) 1716 WP_032470621.1 type IV pilus secretin PilQ Machinery gene
  GPY13_RS13235 pilP 2872054..2872572 (-) 519 WP_000794696.1 pilus assembly protein PilP Machinery gene
  GPY13_RS13240 pilO 2872562..2873152 (-) 591 WP_000135848.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY13_RS13245 pilN 2873145..2873729 (-) 585 WP_000903043.1 PilN domain-containing protein Machinery gene
  GPY13_RS13250 pilM 2873719..2874735 (-) 1017 WP_000520765.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62353.35 Da        Isoelectric Point: 4.5735

>NTDB_id=408465 GPY13_RS13230 WP_032470621.1 2870313..2872028(-) (pilQ) [Vibrio mimicus strain 06-2455]
MRNGLKTYVAQTWLTLWAGLALCASSVAISAESASANQLENIDFRVNKDKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVQSVEVFRKEPSTQLIATIEGEFKHDYTLKGKYLEVVISKLKADEKPQPKSILEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVSGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARMAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSDRGTISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLDELGVRWGVMSTNGSHSVGGTIESNLWQQGLLAEDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SDTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATTVSFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=408465 GPY13_RS13230 WP_032470621.1 2870313..2872028(-) (pilQ) [Vibrio mimicus strain 06-2455]
ATGAGAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTATGGGCAGGCTTGGCACTTTGTGCTTCTTCCGT
GGCGATTAGCGCTGAATCAGCTTCCGCCAATCAACTAGAAAACATTGATTTTCGGGTCAATAAAGACAAAGCGGCTGTGT
TGATTGTCGAATTGGCTTCGCCATCAGCGGTGGTGGATGTACAAAAAGTCCAAGAAGGGCTGAGTATTGAACTCCTGAAA
ACCGATGTGGCAGACGACAAACTCTATTTATTGGATGTGAAGGATTTCTCTACACCAGTACAAAGCGTAGAAGTATTCCG
TAAAGAACCCAGCACTCAGCTTATCGCAACCATCGAAGGTGAGTTTAAGCATGACTACACCTTAAAAGGGAAATACCTTG
AGGTGGTAATCAGTAAGCTCAAAGCAGACGAGAAGCCTCAGCCTAAAAGTATTTTGGAAAAAGAGGGCAAGCTGATCTCG
ATTAACTTCCAAGACATTCCAGTGCGTAACGTACTGCAGTTGATTGCTGATTATAACGGTTTCAACCTCGTTGTTTCAGA
TTCAGTCTCGGGCAATCTGACTTTGCGTTTGGATGGTGTACCTTGGCAGCAAGTGCTAGATATTATCCTGCAGGTTAAAG
GGCTTGATAAACGCGTTGATGGTAACGTAATCCTGATTGCGCCAAAAGAAGAGCTGGATTTGCGTGAGAAACAAGCGTTA
GAAAAAGCACGTATGGCAGAAGAGTTAGGTGATTTAAAATCTGAAATCATCAAAATTAATTTTGCCAAAGCATCTGATAT
CGCAGCAATGATTGGTGGTGAAGGTAACGTCAATATGCTGTCTGATCGTGGCACGATCAGTATTGATGAGCGTACTAACT
CACTGCTGATTCGCGAATTGCCCGATAATATCGCGGTGATCCGTGAAATCATCGAATCGCTAGATATTCCGGTCAAGCAA
GTTCAGATTGAAGCACGTATTGTCACCGTAAAAGAAGGTAACTTGGATGAGCTTGGTGTGCGTTGGGGTGTAATGTCAAC
CAACGGCAGCCACTCTGTGGGTGGAACCATTGAAAGTAACTTATGGCAACAAGGACTGTTGGCGGAAGATGAGTTTCCAG
TTGATGAATTTTTGAATGTTAACTTGGCCTCTACGTCCGCTAATGCCTCTTCTATCGCTTTCCAAGTTGCTAAGTTGGGC
TCAGATACCTTATTGGATTTGGAGTTATCTGCGTTACAAAATGAATCGAAGGCAGAAATCATTTCTAGCCCTCGTTTGAT
TACGACCAATAAACAGCCTGCGTATATTGAACAAGGTACTGAAATTCCTTATCTCGAATCTTCATCCAGTGGTGCAACAA
CCGTATCGTTCAAGAAAGCCGTGTTGAGCTTGAAAGTGACACCACAAATTACGCCGGATAATCGTTTAGTACTGGATTTG
AGTGTGACTCAGGACCGTCGTGGCGAAACGGTGAAAACAGGAACAGGTGAAGCGGTTTCGATCGATACCCAGCGTATTGG
AACACAAGTTCTGGTCAATAATGGTGAAACCGTCGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCAGTGGATA
AGGTGCCATTGCTAGGTGATCTCCCTGTTTTAGGTGCGTTGTTTAGACGAACTTACGAACAAATGGGCAAAAGTGAGCTG
CTGATTTTCGTCACACCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)

Secretin and TonB N terminus short domain (STN)

(181-229)

Bacterial type II and III secretion system protein (Secretin)

(411-569)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

95.447

100

0.954
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

95.447

100

0.954
  pilQ Vibrio campbellii strain DS40M4

78.766

96.497

0.76
  pilQ Legionella pneumophila strain ERS1305867

43.358

94.921

0.412
  pilQ Legionella pneumophila strain Lp02

43.358

94.921

0.412
  comQ Acinetobacter baylyi ADP1

39.446

100

0.399
  pilQ Acinetobacter baumannii strain A118

38.33

100

0.394
  pilQ Acinetobacter baumannii D1279779

38.33

100

0.394
  pilQ Pseudomonas aeruginosa PAK

39.785

97.723

0.389

Multiple sequence alignment