Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY13_RS13250 Genome accession   NZ_CP046823
Coordinates   2873719..2874735 (-) Length   338 a.a.
NCBI ID   WP_000520765.1    Uniprot ID   -
Organism   Vibrio mimicus strain 06-2455     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2868719..2879735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY13_RS13225 aroK 2869602..2870126 (-) 525 WP_000818614.1 shikimate kinase AroK -
  GPY13_RS13230 pilQ 2870313..2872028 (-) 1716 WP_032470621.1 type IV pilus secretin PilQ Machinery gene
  GPY13_RS13235 pilP 2872054..2872572 (-) 519 WP_000794696.1 pilus assembly protein PilP Machinery gene
  GPY13_RS13240 pilO 2872562..2873152 (-) 591 WP_000135848.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY13_RS13245 pilN 2873145..2873729 (-) 585 WP_000903043.1 PilN domain-containing protein Machinery gene
  GPY13_RS13250 pilM 2873719..2874735 (-) 1017 WP_000520765.1 type IV pilus assembly protein PilM Machinery gene
  GPY13_RS13255 - 2874877..2877354 (+) 2478 WP_000668749.1 penicillin-binding protein 1A -
  GPY13_RS13260 oxyR 2877421..2878314 (-) 894 WP_001025915.1 DNA-binding transcriptional regulator OxyR -
  GPY13_RS13265 - 2878454..2879185 (+) 732 WP_000495217.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37593.24 Da        Isoelectric Point: 7.6008

>NTDB_id=408469 GPY13_RS13250 WP_000520765.1 2873719..2874735(-) (pilM) [Vibrio mimicus strain 06-2455]
MGKTLVTGIDIGHHSIKAVVLKPMGDTYALMGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDNA
VISKVLQIDSDLERREQEFAIYQAFSHQSPFPVEDLSLDFVKVAEKNIARSSTSTFQVYATKKEVVESRLQASKKAGFEP
ILMDVQVHSLLHLWQLASRAYRRPDWMLIDIGHTQSSLCFDFAEKMPFYKDVPLGTRVLGAEPSSTPVLGDSLMQDPTQR
FVNEFVDKVARQIQLFTSVHGAQSLGGLWLSGGGATIAGLEEALHQRLSLPCEVLNPFSLFKTNVSRRKRQVADGQRFST
AAGLALRGLAWLESEHAA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=408469 GPY13_RS13250 WP_000520765.1 2873719..2874735(-) (pilM) [Vibrio mimicus strain 06-2455]
ATGGGTAAAACATTAGTTACGGGTATTGATATCGGCCACCACAGCATAAAAGCCGTAGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTATGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGGTTGCCCTTATTTAGTCACAAAGTCGCGATTGCAATTCCGGATAACGCT
GTAATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCGTCGAGAACAGGAATTTGCCATCTATCAGGCCTTCTCTCA
TCAATCTCCATTTCCCGTTGAAGATCTTAGCCTCGATTTCGTCAAAGTGGCGGAAAAAAATATCGCGCGCAGTAGTACAA
GCACCTTCCAAGTCTATGCCACGAAAAAAGAAGTAGTGGAAAGCCGCTTACAAGCGAGTAAGAAAGCGGGGTTTGAGCCG
ATTTTGATGGATGTGCAAGTCCATAGCTTGCTCCATTTATGGCAGCTCGCTAGCCGTGCCTATCGACGTCCGGATTGGAT
GCTGATTGATATTGGCCATACCCAGAGTTCATTGTGTTTCGACTTTGCAGAAAAAATGCCCTTTTACAAAGATGTCCCAT
TGGGTACGCGAGTGTTGGGCGCAGAGCCGAGCAGCACTCCCGTATTAGGCGATTCATTAATGCAGGACCCCACTCAGCGT
TTTGTTAATGAATTCGTGGATAAGGTTGCGCGCCAAATTCAGTTGTTTACCTCCGTACATGGAGCTCAAAGTCTTGGCGG
TTTATGGCTTTCCGGCGGCGGTGCAACCATTGCTGGACTTGAGGAGGCGTTACACCAACGTTTATCACTTCCCTGTGAAG
TGTTGAATCCATTCTCACTATTCAAAACGAATGTCTCAAGACGCAAGCGCCAAGTGGCTGATGGGCAGCGCTTTAGCACT
GCCGCTGGCCTTGCATTGCGTGGTTTAGCATGGTTGGAGAGTGAACATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-215)

Type IV pilus assembly protein PilM; (PilM_2)

(233-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

85.503

100

0.855
  pilM Vibrio campbellii strain DS40M4

49.408

100

0.494

Multiple sequence alignment