Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPX92_RS11130 Genome accession   NZ_CP046802
Coordinates   1450366..1450791 (-) Length   141 a.a.
NCBI ID   WP_158117120.1    Uniprot ID   -
Organism   Vibrio cincinnatiensis strain 2070-81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1445366..1455791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX92_RS11110 coaE 1445897..1446511 (-) 615 WP_158117118.1 dephospho-CoA kinase -
  GPX92_RS11115 pilD 1446517..1447383 (-) 867 WP_078926030.1 prepilin peptidase Machinery gene
  GPX92_RS11120 pilC 1447424..1448650 (-) 1227 WP_158117119.1 type II secretion system F family protein Machinery gene
  GPX92_RS11125 pilB 1448671..1450359 (-) 1689 WP_078926032.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPX92_RS11130 pilA 1450366..1450791 (-) 426 WP_158117120.1 pilin Machinery gene
  GPX92_RS11135 nadC 1450988..1451878 (-) 891 WP_154115270.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPX92_RS11140 ampD 1452076..1452621 (+) 546 WP_078926149.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPX92_RS11145 fldB 1452628..1453146 (-) 519 WP_078926035.1 flavodoxin FldB -
  GPX92_RS11150 xerD 1453290..1454198 (+) 909 WP_078926036.1 site-specific tyrosine recombinase XerD -
  GPX92_RS11155 dsbC 1454214..1454957 (+) 744 WP_078926037.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15117.04 Da        Isoelectric Point: 7.1316

>NTDB_id=408066 GPX92_RS11130 WP_158117120.1 1450366..1450791(-) (pilA) [Vibrio cincinnatiensis strain 2070-81]
MKKNPNHKQQGFTLIELMIVVAIIGVLSAIAVPAYKDYVTKSELASGFATIKSVITPAELYVQENGKISGGANTTDTLGV
KNDANSLGELTIPNDNEIQFEHKNGSAEGAKFTYKRENGGWKCAYVAPNNNQNADAPKGCQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=408066 GPX92_RS11130 WP_158117120.1 1450366..1450791(-) (pilA) [Vibrio cincinnatiensis strain 2070-81]
ATGAAAAAGAATCCCAACCATAAACAACAAGGTTTTACCTTAATTGAACTGATGATTGTGGTCGCAATCATTGGCGTTTT
ATCCGCCATTGCTGTTCCCGCCTATAAGGACTACGTAACGAAAAGTGAGTTGGCTTCTGGGTTTGCTACCATAAAATCCG
TTATCACTCCCGCTGAGCTTTATGTACAGGAAAATGGCAAGATCAGTGGTGGAGCAAATACAACCGATACACTCGGAGTA
AAAAACGATGCCAACTCTTTGGGGGAACTCACCATTCCTAACGATAATGAAATCCAATTTGAGCATAAGAACGGCTCAGC
AGAAGGCGCTAAATTTACTTATAAAAGAGAAAATGGTGGTTGGAAATGTGCTTATGTTGCACCAAACAACAATCAAAACG
CTGATGCACCGAAAGGTTGCCAATAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

48.276

100

0.496
  pilA Pseudomonas aeruginosa PAK

38.926

100

0.411
  pilA Vibrio cholerae strain A1552

37.748

100

0.404
  pilA Vibrio cholerae C6706

37.748

100

0.404
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.748

100

0.404
  pilA Ralstonia pseudosolanacearum GMI1000

33.951

100

0.39
  pilE Neisseria gonorrhoeae MS11

34.615

100

0.383

Multiple sequence alignment