Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPX92_RS11125 Genome accession   NZ_CP046802
Coordinates   1448671..1450359 (-) Length   562 a.a.
NCBI ID   WP_078926032.1    Uniprot ID   -
Organism   Vibrio cincinnatiensis strain 2070-81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1443671..1455359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX92_RS11100 yacG 1444876..1445073 (-) 198 WP_158117117.1 DNA gyrase inhibitor YacG -
  GPX92_RS11105 zapD 1445131..1445871 (-) 741 WP_078926028.1 cell division protein ZapD -
  GPX92_RS11110 coaE 1445897..1446511 (-) 615 WP_158117118.1 dephospho-CoA kinase -
  GPX92_RS11115 pilD 1446517..1447383 (-) 867 WP_078926030.1 prepilin peptidase Machinery gene
  GPX92_RS11120 pilC 1447424..1448650 (-) 1227 WP_158117119.1 type II secretion system F family protein Machinery gene
  GPX92_RS11125 pilB 1448671..1450359 (-) 1689 WP_078926032.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPX92_RS11130 pilA 1450366..1450791 (-) 426 WP_158117120.1 pilin Machinery gene
  GPX92_RS11135 nadC 1450988..1451878 (-) 891 WP_154115270.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPX92_RS11140 ampD 1452076..1452621 (+) 546 WP_078926149.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPX92_RS11145 fldB 1452628..1453146 (-) 519 WP_078926035.1 flavodoxin FldB -
  GPX92_RS11150 xerD 1453290..1454198 (+) 909 WP_078926036.1 site-specific tyrosine recombinase XerD -
  GPX92_RS11155 dsbC 1454214..1454957 (+) 744 WP_078926037.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62413.28 Da        Isoelectric Point: 5.1427

>NTDB_id=408065 GPX92_RS11125 WP_078926032.1 1448671..1450359(-) (pilB) [Vibrio cincinnatiensis strain 2070-81]
MLTNLIAILRQAQLMSLAQEQAVVDHIQTSGASVPEALLALDIFHHHDLTEHLSQIFGLPETDVTRFDYASLCQQLGLRE
LITQYQALPLQRNGQVLTLAVSDPTHLQIEDEFRFATGLQIELVLADYKALQAAIRRLYGRTINHATAQGKEISQDELAD
LVQISDDEKQSVEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYEQQYRIRLRCDGILVQIQQPASHLGRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSSASLDIDKLGYNDTQKQLYLNALKQPQGMI
LMTGPTGSGKTVSLYTGLSILNTEDVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKEAYFPSLTEQQQFALDADQ
TIYQAHSNGCNECTNGYSGRVGIYEVMAFEPQLAEAIMQKASITQIEAIAQTNGMQTLQQSGIEKLKIGITSIQELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=408065 GPX92_RS11125 WP_078926032.1 1448671..1450359(-) (pilB) [Vibrio cincinnatiensis strain 2070-81]
ATGCTTACCAACCTTATTGCTATCTTGCGACAAGCTCAACTTATGAGTTTGGCGCAAGAGCAAGCCGTGGTCGACCATAT
CCAAACTTCCGGTGCCAGTGTACCGGAAGCTTTATTGGCTCTGGATATTTTTCACCACCATGATTTAACCGAACACCTAA
GCCAAATATTTGGTCTGCCCGAAACCGATGTAACTCGCTTTGACTACGCTTCATTGTGTCAACAGCTGGGTTTGCGAGAA
CTAATAACACAATACCAAGCCTTACCATTACAGCGGAACGGACAGGTACTGACCTTAGCCGTATCCGACCCCACACATCT
ACAAATTGAAGATGAATTTCGCTTTGCCACCGGGCTACAAATTGAACTAGTACTGGCGGACTATAAAGCCTTACAAGCGG
CCATTCGTAGATTGTATGGTCGAACAATCAACCATGCTACGGCGCAAGGTAAAGAGATCAGTCAAGATGAACTGGCTGAT
TTGGTACAGATCTCGGATGATGAAAAGCAATCCGTTGAAGATCTCAGCCAAGATGACTCTCCAGTGAGTCGCTTTATCAA
CCAAATATTATTGGATGCCGTCCGTAAAGGGGCCTCCGACATACACTTTGAGCCTTACGAACAGCAATACCGCATTCGCC
TACGCTGTGACGGAATTCTGGTGCAGATCCAGCAGCCCGCAAGCCACTTAGGACGACGGCTCGCTGCACGGATAAAAATT
CTCTCTAAACTGGATATTGCTGAACGCCGGCTCCCACAAGACGGGCGAATAAAACTGCGCTTAAATGCAGATACCGCCAT
CGATATGCGAGTTTCAACCCTTCCTACATTATGGGGAGAGAAAATTGTCCTGCGCTTACTCGATAGCAGTAGCGCAAGTT
TAGACATTGATAAGTTAGGTTATAACGATACACAAAAACAGCTTTACCTAAACGCGTTGAAACAGCCGCAAGGGATGATC
CTGATGACGGGGCCAACAGGAAGTGGCAAAACGGTTTCACTTTATACAGGCTTAAGCATTCTTAATACTGAAGATGTTAA
TATCTCTACCGCAGAAGATCCGGTGGAGATCAACCTTCCTGGCATTAATCAAGTCCAAGTTCAACCCAAAATTGGCTTTG
GCTTTGCCCAAGCGCTACGCTCATTTTTGCGTCAAGACCCTGATATTGTCATGGTTGGTGAAATCCGCGATTTAGAAACC
GCAGAAATCGCAGTCAAAGCCGCTCAAACAGGACACTTGGTTCTGTCAACGTTACACACCAACTCAGCCGCAGAAACCGT
CATTCGCTTAGCCAATATGGGCATAGAACCCTTTAACCTCGCCTCTTCACTCAGTTTAATCATTGCTCAGCGCTTAGCCA
GACGGTTATGTCCACACTGCAAAGAAGCCTATTTTCCAAGCTTAACCGAGCAACAACAGTTTGCGTTAGACGCCGATCAA
ACCATTTACCAAGCCCATTCGAATGGCTGTAACGAATGCACCAATGGTTACAGTGGACGAGTCGGTATTTATGAAGTGAT
GGCCTTTGAGCCTCAACTGGCTGAAGCCATTATGCAAAAAGCTTCCATTACTCAAATTGAAGCCATCGCTCAAACCAATG
GCATGCAAACTTTGCAGCAATCTGGCATAGAAAAACTCAAAATCGGCATCACCAGTATTCAAGAATTACAGCGTGTACTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

81.673

100

0.817
  pilB Vibrio campbellii strain DS40M4

72.242

100

0.722
  pilB Vibrio parahaemolyticus RIMD 2210633

70.641

100

0.706
  pilB Legionella pneumophila strain ERS1305867

52.724

91.459

0.482
  pilB Acinetobacter baumannii D1279779

51.257

91.993

0.472
  pilB Acinetobacter baylyi ADP1

51.262

91.637

0.47
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.45
  pilB/pilB1 Synechocystis sp. PCC 6803

38.364

97.865

0.375

Multiple sequence alignment