Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY18_RS17405 Genome accession   NZ_CP046800
Coordinates   2215700..2216119 (-) Length   139 a.a.
NCBI ID   WP_047459095.1    Uniprot ID   A0AAX2LK83
Organism   Vibrio fluvialis strain 2012V-1235     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2210700..2221119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY18_RS17385 coaE 2211208..2211816 (-) 609 WP_158147130.1 dephospho-CoA kinase -
  GPY18_RS17390 pilD 2211819..2212688 (-) 870 WP_020332198.1 A24 family peptidase Machinery gene
  GPY18_RS17395 pilC 2212740..2213966 (-) 1227 WP_020332199.1 type II secretion system F family protein Machinery gene
  GPY18_RS17400 pilB 2214002..2215690 (-) 1689 WP_158147131.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY18_RS17405 pilA 2215700..2216119 (-) 420 WP_047459095.1 pilin Machinery gene
  GPY18_RS17410 nadC 2216351..2217238 (-) 888 WP_104966496.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY18_RS17415 ampD 2217391..2217942 (+) 552 WP_158147132.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY18_RS17420 fldB 2217947..2218468 (-) 522 WP_020430452.1 flavodoxin FldB -
  GPY18_RS17425 xerD 2218663..2219517 (+) 855 WP_230857653.1 site-specific tyrosine recombinase XerD -
  GPY18_RS17430 dsbC 2219534..2220298 (+) 765 WP_024374397.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14270.22 Da        Isoelectric Point: 8.4642

>NTDB_id=408027 GPY18_RS17405 WP_047459095.1 2215700..2216119(-) (pilA) [Vibrio fluvialis strain 2012V-1235]
MNANQRKQQQGFTLIELMIVVAIIGVLSAIAVPAYKDYVTKSELASGFATIKSVLTPAELYVQENGKISSGVSTITSLGV
SSAANSLGTLSISADNEITFSHTDGSAKGAKFVYTRTTGGWSCAYTGPAAVSAAAPKGC

Nucleotide


Download         Length: 420 bp        

>NTDB_id=408027 GPY18_RS17405 WP_047459095.1 2215700..2216119(-) (pilA) [Vibrio fluvialis strain 2012V-1235]
ATGAACGCGAATCAACGCAAACAACAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTCGCCATCATCGGAGTTCT
GTCGGCGATTGCCGTACCCGCTTATAAGGATTACGTAACCAAGAGTGAGTTAGCTTCTGGCTTTGCCACAATTAAATCCG
TGCTAACACCAGCAGAGTTATATGTCCAAGAAAATGGAAAGATTAGTAGTGGAGTAAGTACCATCACATCGCTGGGTGTT
TCCTCTGCCGCTAATAGCTTAGGCACACTAAGTATCTCAGCAGACAATGAAATCACCTTTTCTCACACTGATGGTTCAGC
AAAGGGCGCGAAGTTCGTATACACAAGAACAACTGGTGGCTGGTCATGTGCGTACACCGGACCAGCAGCCGTGAGCGCAG
CAGCACCGAAAGGTTGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

50.746

96.403

0.489
  pilA Acinetobacter baumannii strain A118

41.83

100

0.46
  pilA Pseudomonas aeruginosa PAK

40.667

100

0.439
  pilA Vibrio cholerae C6706

38.312

100

0.424
  pilA Vibrio cholerae strain A1552

38.312

100

0.424
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.312

100

0.424
  pilA2 Legionella pneumophila str. Paris

37.857

100

0.381
  pilA2 Legionella pneumophila strain ERS1305867

37.143

100

0.374
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.31

92.806

0.374

Multiple sequence alignment