Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY18_RS17400 Genome accession   NZ_CP046800
Coordinates   2214002..2215690 (-) Length   562 a.a.
NCBI ID   WP_158147131.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2012V-1235     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2209002..2220690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY18_RS17375 yacG 2210183..2210380 (-) 198 WP_020332195.1 DNA gyrase inhibitor YacG -
  GPY18_RS17380 zapD 2210435..2211175 (-) 741 WP_020332196.1 cell division protein ZapD -
  GPY18_RS17385 coaE 2211208..2211816 (-) 609 WP_158147130.1 dephospho-CoA kinase -
  GPY18_RS17390 pilD 2211819..2212688 (-) 870 WP_020332198.1 A24 family peptidase Machinery gene
  GPY18_RS17395 pilC 2212740..2213966 (-) 1227 WP_020332199.1 type II secretion system F family protein Machinery gene
  GPY18_RS17400 pilB 2214002..2215690 (-) 1689 WP_158147131.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY18_RS17405 pilA 2215700..2216119 (-) 420 WP_047459095.1 pilin Machinery gene
  GPY18_RS17410 nadC 2216351..2217238 (-) 888 WP_104966496.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY18_RS17415 ampD 2217391..2217942 (+) 552 WP_158147132.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY18_RS17420 fldB 2217947..2218468 (-) 522 WP_020430452.1 flavodoxin FldB -
  GPY18_RS17425 xerD 2218663..2219517 (+) 855 WP_230857653.1 site-specific tyrosine recombinase XerD -
  GPY18_RS17430 dsbC 2219534..2220298 (+) 765 WP_024374397.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62037.96 Da        Isoelectric Point: 6.2645

>NTDB_id=408026 GPY18_RS17400 WP_158147131.1 2214002..2215690(-) (pilB) [Vibrio fluvialis strain 2012V-1235]
MLTNLLAILRQTDLLSPTQEQAVMDHIHASGTNVPEALIDLAIFHHHELTEHLSQIFGLPNTDLTRFDFAPLCQQLGLRE
LITRYQALPLNSNGQVLTLAVSDPTHLQVEDEFRFATGLQIELVLADHKALQAAIRRLYGRAITSHGTQGKEISQDELAS
LVQVSDDEKTSIEDLSQDDAPVSRFINQILLDAVRKGASDIHFEPYESFYRIRLRCDGILVEIQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSASSLDIDKLGYNDAQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTDEVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAESVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVATSPSPTLQQQFHLSSQQ
PIYAANPSGCNECTNGYSGRVGIYEVMAFNSALAEAIMQRASIHQIESIAQQHGMQTLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=408026 GPY18_RS17400 WP_158147131.1 2214002..2215690(-) (pilB) [Vibrio fluvialis strain 2012V-1235]
ATGCTCACCAACCTACTCGCCATCCTGCGTCAGACCGATTTGCTTAGCCCTACGCAGGAACAGGCCGTGATGGATCATAT
TCACGCTTCCGGAACGAATGTCCCGGAAGCGTTGATCGATTTGGCGATCTTCCATCACCATGAACTGACCGAGCATCTAA
GCCAGATCTTTGGTCTGCCGAATACGGATCTGACCCGTTTTGACTTCGCGCCGCTGTGCCAGCAGTTGGGGCTGCGCGAG
CTCATCACCCGTTATCAGGCTCTGCCCCTCAACAGCAACGGTCAGGTACTGACTCTTGCGGTATCCGATCCTACTCACCT
ACAAGTTGAAGACGAGTTTCGTTTTGCGACCGGGTTACAAATAGAACTGGTATTGGCTGATCATAAGGCACTGCAAGCCG
CTATTCGCCGCTTGTATGGCCGAGCCATCACGTCACATGGTACGCAGGGCAAGGAGATCAGCCAGGACGAACTGGCCAGT
CTGGTGCAGGTTTCCGATGACGAAAAAACATCAATCGAAGATCTGAGTCAGGATGACGCCCCGGTCAGTCGTTTCATCAA
CCAGATCCTGCTGGATGCGGTGCGTAAAGGCGCTTCTGATATCCACTTTGAGCCCTATGAATCCTTTTATCGCATTCGCC
TGCGCTGCGACGGTATTTTGGTGGAAATCCAGCAACCAGCCAGCCATTTAAGCCGTCGACTGGCCGCGCGGATTAAGATT
TTGTCGAAGCTCGATATCGCCGAGCGACGTTTACCCCAGGACGGCCGCATCAAATTACGCCTCAATGCGGACACGGCCAT
TGATATGCGGGTCTCCACTCTGCCAACCTTGTGGGGGGAGAAAATCGTCCTGCGTTTGCTCGACAGCAGTGCGTCCAGCC
TCGATATCGACAAGTTGGGTTATAACGATGCGCAAAAGCAGCTTTATCTGGCGGCCCTCACCAAGCCGCAAGGCATGATT
CTGATGACTGGCCCGACGGGCAGCGGTAAGACAGTCTCACTCTATACCGGCCTTCGCATTCTCAACACCGACGAAGTGAA
TATTTCTACCGCCGAAGACCCGGTGGAAATTAACCTGCCCGGCATTAACCAGGTTCAGGTGCAGCCCAAAATTGGTTTTG
GCTTTGCGCAGGCTCTGCGCTCGTTTCTGCGTCAGGATCCCGATGTGGTGATGGTGGGCGAAATCCGCGATTTGGAAACG
GCAGAGATTGCCGTCAAAGCCGCCCAAACCGGCCACTTGGTGCTATCCACACTGCACACCAACTCCGCCGCCGAAAGCGT
GATTCGGCTGGCGAACATGGGCATTGAACCGTTTAACCTCGCTTCATCGCTGAGCCTCATCATCGCTCAACGACTTGCCC
GGCGCCTTTGCCCTCACTGCAAAGTCGCCACATCGCCCTCTCCGACACTGCAGCAACAATTTCACTTGAGTTCTCAGCAG
CCTATCTATGCCGCCAACCCATCAGGCTGCAACGAGTGCACCAACGGTTACAGCGGGCGGGTGGGAATTTATGAAGTGAT
GGCATTTAACTCCGCGCTGGCTGAAGCGATTATGCAACGAGCCAGCATCCATCAAATCGAATCCATTGCCCAGCAACATG
GCATGCAAACGCTGTTGCAATCGGGGATCGAAAAACTTCGCGCCGGGATCACCAGTTTCAGTGAGTTGCAGCGTGTTCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.384

100

0.824
  pilB Vibrio campbellii strain DS40M4

72.242

100

0.722
  pilB Vibrio parahaemolyticus RIMD 2210633

71.53

100

0.715
  pilB Legionella pneumophila strain ERS1305867

52.805

91.993

0.486
  pilB Acinetobacter baumannii D1279779

50.096

92.349

0.463
  pilB Acinetobacter baylyi ADP1

48.767

93.772

0.457
  pilF Neisseria gonorrhoeae MS11

44.504

100

0.447
  pilF Thermus thermophilus HB27

37.367

100

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

37.818

97.865

0.37

Multiple sequence alignment