Detailed information    

insolico Bioinformatically predicted

Overview

Name   comP   Type   Machinery gene
Locus tag   GNX71_RS32845 Genome accession   NZ_CP046508
Coordinates   7043623..7044063 (-) Length   146 a.a.
NCBI ID   WP_206176253.1    Uniprot ID   -
Organism   Variovorax sp. RKNM96     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7038623..7049063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNX71_RS32820 (GNX71_32745) - 7039988..7040578 (-) 591 WP_206176249.1 PilX N-terminal domain-containing pilus assembly protein -
  GNX71_RS32825 (GNX71_32750) - 7040575..7041429 (-) 855 WP_206176250.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GNX71_RS32830 (GNX71_32755) pilV 7041434..7041967 (-) 534 WP_206176251.1 type IV pilus modification protein PilV -
  GNX71_RS32835 (GNX71_32760) - 7041998..7042483 (-) 486 WP_206176252.1 GspH/FimT family pseudopilin -
  GNX71_RS33650 - 7042712..7042939 (-) 228 WP_277401878.1 Wzy polymerase domain-containing protein -
  GNX71_RS32845 (GNX71_32770) comP 7043623..7044063 (-) 441 WP_206176253.1 pilin Machinery gene
  GNX71_RS32850 (GNX71_32775) - 7044270..7045073 (-) 804 WP_206179819.1 3',5'-cyclic-nucleotide phosphodiesterase -
  GNX71_RS32855 (GNX71_32780) - 7045127..7047379 (-) 2253 WP_206176254.1 adenylate/guanylate cyclase domain-containing protein -
  GNX71_RS32860 (GNX71_32785) - 7047428..7048081 (-) 654 WP_206176255.1 FHA domain-containing protein -
  GNX71_RS32865 (GNX71_32790) - 7048151..7048960 (-) 810 WP_206176256.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14790.23 Da        Isoelectric Point: 8.8696

>NTDB_id=404782 GNX71_RS32845 WP_206176253.1 7043623..7044063(-) (comP) [Variovorax sp. RKNM96]
MNVRKIARRAQAGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVSEAMVAASAAKATIGENIASAAGVIDANSCNGVT
NSTVATENVKSLVCASGVLTLTGTTKAGDASVVLTPLVNADKVVTWGCVGTPAKYVPAQCRAAVTP

Nucleotide


Download         Length: 441 bp        

>NTDB_id=404782 GNX71_RS32845 WP_206176253.1 7043623..7044063(-) (comP) [Variovorax sp. RKNM96]
ATGAACGTTCGCAAGATCGCCCGCCGCGCACAGGCCGGCTTTACCCTTATCGAATTGATGATCGTTGTGGCGATCATTGG
TATCTTGGCTGCTGTGGCTCTGCCGGCTTATCAGGACTACACAGTTCGTGCACGTGTATCGGAAGCCATGGTCGCCGCTT
CCGCCGCGAAGGCAACGATCGGCGAAAACATCGCTTCTGCCGCCGGTGTGATTGATGCCAACTCTTGCAACGGTGTCACG
AACAGCACCGTCGCCACAGAAAACGTTAAAAGTCTCGTTTGCGCAAGTGGTGTGCTGACACTGACTGGCACCACAAAGGC
AGGTGACGCATCCGTGGTCCTGACGCCGTTGGTTAACGCGGACAAAGTTGTGACATGGGGCTGCGTGGGCACGCCTGCAA
AGTATGTTCCCGCTCAGTGCCGCGCAGCTGTTACCCCCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comP Acinetobacter baylyi ADP1

50

100

0.5
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.602

100

0.466
  pilA2 Legionella pneumophila str. Paris

50.746

91.781

0.466
  pilA2 Legionella pneumophila strain ERS1305867

50

91.781

0.459
  pilA Ralstonia pseudosolanacearum GMI1000

39.759

100

0.452
  pilE Neisseria gonorrhoeae strain FA1090

38.71

100

0.411
  pilA Acinetobacter baumannii strain A118

38.356

100

0.384
  pilA Haemophilus influenzae 86-028NP

38.732

97.26

0.377
  pilA Haemophilus influenzae Rd KW20

38.732

97.26

0.377
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.194

98.63

0.377
  pilA/pilA1 Eikenella corrodens VA1

34.868

100

0.363

Multiple sequence alignment