Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GMO17_RS21635 Genome accession   NZ_CP046441
Coordinates   4795562..4796779 (+) Length   405 a.a.
NCBI ID   WP_024690386.1    Uniprot ID   A0AA40NZE6
Organism   Pseudomonas coronafaciens pv. coronafaciens strain B19001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4790562..4801779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GMO17_RS21610 (GMO17_21945) - 4791111..4791656 (+) 546 WP_122354618.1 DUF1523 family protein -
  GMO17_RS21615 (GMO17_21950) - 4791700..4792107 (-) 408 WP_122354616.1 hypothetical protein -
  GMO17_RS21620 (GMO17_21955) - 4792104..4792691 (-) 588 WP_183136699.1 DUF433 domain-containing protein -
  GMO17_RS21625 (GMO17_21960) - 4793234..4793638 (-) 405 WP_005895657.1 pilin -
  GMO17_RS21630 (GMO17_21965) pilB 4793865..4795559 (+) 1695 WP_005895655.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GMO17_RS21635 (GMO17_21970) pilC 4795562..4796779 (+) 1218 WP_024690386.1 type II secretion system F family protein Machinery gene
  GMO17_RS21640 (GMO17_21975) pilD 4796781..4797653 (+) 873 WP_122347408.1 prepilin peptidase Machinery gene
  GMO17_RS21645 (GMO17_21980) coaE 4797650..4798273 (+) 624 WP_122347409.1 dephospho-CoA kinase -
  GMO17_RS21650 (GMO17_21985) yacG 4798270..4798479 (+) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  GMO17_RS21655 (GMO17_21990) - 4798623..4799252 (+) 630 WP_005895647.1 DUF1780 domain-containing protein -
  GMO17_RS21660 (GMO17_21995) - 4799249..4799782 (+) 534 WP_053481610.1 MOSC domain-containing protein -
  GMO17_RS21665 (GMO17_22000) - 4800000..4801298 (+) 1299 WP_122311686.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44244.23 Da        Isoelectric Point: 10.2266

>NTDB_id=404298 GMO17_RS21635 WP_024690386.1 4795562..4796779(+) (pilC) [Pseudomonas coronafaciens pv. coronafaciens strain B19001]
MASKAAKVIVFIWEGVDKKGTKTSGELSGHNLALVKAQLRKQGINPTKVRKKSASIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRVLVNSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPIAVLIVAMIVSGILLIKVVPQFQSVFASFGAQLPTFTLMVIGLSDVVQKWWLAIVGLFFVSFFLFK
RAYKQSQKFRDTLDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDTMLDKVATYYEDEVDNMVDNLTSLMEPMIMAFLGVIVGGLVIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=404298 GMO17_RS21635 WP_024690386.1 4795562..4796779(+) (pilC) [Pseudomonas coronafaciens pv. coronafaciens strain B19001]
ATGGCCAGCAAGGCAGCCAAAGTCATCGTTTTCATTTGGGAAGGGGTTGACAAGAAAGGCACTAAAACCAGTGGAGAGCT
GAGCGGCCATAATCTGGCACTCGTAAAAGCCCAACTGCGTAAGCAAGGGATCAACCCTACAAAGGTGCGCAAGAAATCGG
CGTCCATCTTTGGTAAGGGCAAGAAAATCAAGCCTCTGGACATCGCGTTCTTTTCTCGGCAGATGGCGACAATGATGAAG
GCAGGCGTGCCCCTGCTTCAGTCATTCGATATTATCAGCGAGGGTGCAGAGAATCCGAATATGCGAGTGCTGGTAAATTC
GCTCAAACAGGAAGTTTCAGCGGGTAATAGTTTTGCAACCGCCTTGCGACAAAAGCCCGAGTATTTCGACGATCTTTTTT
GCAACCTGGTAGACGCCGGCGAGCAAGCTGGCGCACTTGAAAGCCTGCTGGACCGGGTTGCGAGCTACAAAGAAAAGACC
GAAAAGCTAAAAGCTAAAATCAAAAAAGCCATGACATACCCGATTGCCGTGTTGATCGTCGCAATGATTGTGTCCGGCAT
TTTGCTGATCAAAGTGGTGCCGCAGTTCCAGTCAGTCTTCGCTAGCTTCGGCGCCCAATTGCCCACTTTCACACTGATGG
TAATCGGCCTGTCCGACGTCGTACAAAAATGGTGGCTGGCAATCGTTGGTCTATTTTTCGTCAGCTTTTTTTTGTTCAAA
CGAGCTTATAAGCAGTCTCAAAAATTCCGAGACACCCTCGACCGCTTCTTACTGAAAGTACCTATCATTGGCCCGTTGAT
TTTCAAGTCGTCGGTTGCTCGTTATGCCCGTACGCTTGCTACTACATTCGCTGCTGGCGTTCCGCTTGTAGAGGCTTTGG
ACTCAGTTGCTGGAGCCACGGGCAACGTAGTCTTCAAAAACGCAGTCAATAAGGTAAAGCAAGATGTCTCAACTGGTATG
CAACTGAATTTCTCGATGCGCTCAACAGGAGTATTTCCAAGCCTTGCGATTCAGATGACTGCCATTGGCGAGGAATCCGG
AGCTCTGGACACCATGCTCGACAAAGTTGCTACCTACTATGAGGATGAAGTCGACAACATGGTCGACAATCTGACCAGCC
TCATGGAACCGATGATCATGGCCTTTTTGGGAGTAATCGTCGGCGGGCTGGTCATTGCGATGTATCTACCTATCTTCCAA
CTCGGAAACGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baylyi ADP1

59.804

100

0.602
  pilC Acinetobacter baumannii D1279779

59.559

100

0.6
  pilC Legionella pneumophila strain ERS1305867

51.232

100

0.514
  pilG Neisseria gonorrhoeae MS11

43.719

98.272

0.43
  pilG Neisseria meningitidis 44/76-A

43.467

98.272

0.427
  pilC Vibrio campbellii strain DS40M4

42.26

100

0.425
  pilC Vibrio cholerae strain A1552

41.919

97.778

0.41
  pilC Thermus thermophilus HB27

37.905

99.012

0.375

Multiple sequence alignment